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CRIMIC CR01746

Gene nst

CG10627

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:12529640..12529647	69C4	FBti0210489	86502

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTCAGTTTCAGCATGTTCTCCTCG	CGTAGGCTAATCTCGGATTGTGTGAC

Images

Construct Design Sequences

sgRNA sequence	ACATACTGACGCACAGTCGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGACACCGATGCCCTGATTA	GGCAGCGAACTAGTGCCA	TGTGCGTCAGTATGTATGCAC	TGTGAAATCCCTTCCGTCCG

 

Amplicon 5'

Amplicon 3'

CGACACCGATGCCCTGATTAATGACCGTCACGTTGAGGGTGCCCTTCATGCGCTTGATGAACTGCAGCATCTTGCGCGCACCCACACCGTTGGCACCATCGTAGATGATGCTATTGCGATAGTTGCCGTTCTCCAGGCGCCCGTTGCGCAGCAGTTCGAATGCCTTGATCAGCTTGTCGTAGTAACCCTCCTCGGTGGGCTTGCCGTATGCCTCCTTGGTATTCGCCGCCACCACGAAGTAGTGCAGCATGGGTGTGGTCACAATGCCGTACTCCTTGACGTTACCCTTTAGCGCTATCACGCCATCGGCCACCGCTTTCAGCAGCCGCGGACTGTGGTATCTGTTGTCCATGCCCACAAACACCTGGGAACTGGTCGTCACATCGATATTGTTGTCCTTTATGATCTTGGCCACCTGCTGCTCCAGCTCCTGGTCGCTGACGTTGACCAGATCGGTGGCTATCGCCTCCCAGCTGGCCTCCAGCATCTCGCCCTTGGGATCGACCAGCTTCACTCCGTTGTCCGGCTCCGGATTGTGCGATGCCGTGATCATCACACCGATGACGGAGCCTCCGCGATACCGGGAACGCAGGGTGGCCAGAACGCCCATGCGGAACATCACGCTGTCCAGGAACTCAGCCCTAAAAAAAAAATGCGGAGTTATTACATCAGGATTTTGTTTAGATACAAAAGTATCTTTTAGCTTGTATTTTGGAGTTGGAAAATGTTTTGTACCTAATGTTGTTTGTTTATATGTGTGTTATCACTTTAGCTATCTATTTTATCTTCGCTGGATTGATAAGCCTATAGAAGCACTGTGTATTGCTCATTTTAGGCGTTGCAATTGCTTTTATCAGTAGGTGTGTCATGGAAACTTTGTTATCTGGCTGTAAAGTGGTTAGAAAATAAGGGCGCGGAAACCACAAACTATGTAATCAAATACTTAGTTGGTTCATTTTTCACAAATAACAAGTTATTAATCATTGAGATTTGAAAATATTGCAACTTGCACATGTGGCACTAGTTCGCTGCC

TGTGCGTCAGTATGTATGCACATATGCACATGCACGCACACTTACGCACATACAGGCAGACATAACGGCGAAAGAAGGGCATGTCCATTGATAAAAGGTGAACAATATCTAAAAATAAACGCCAAATTCCAACATCACAGCCCTGCAACCGCCCATTTATCAAAAAAAAAAAAAAACCACCAACAATCGCCGTGTGCTAATCAAATGCGCCAACGTGTGCATCGTGTGGGTGTGTTAGCATAATGATTATGATGATTATTAAGATCGTTAATGGAGGCAACTACTCACTTGCCGCGAAAGCCAGCTGTTCCATATTGGATGGTCTCCTTGGAGGTCTTTGGGTACATCTCGCGGGCGAAGGCGTAGACGGTGCGCAGGTTAATAGACATCTTGCGATACTCGAGGGCGGTGCCACTGGTCCAGAGCGAGGTGAAAGGTTCCGTCCGGTTTTTCCGGACTTTCGAAATTTAACAATTCCACAGATGTGTGCAGGCGGGTGTGACCGTCGTTTCGCGGCACTGTTCCACCCTTGCTTATGCGGCCAAGCGGTCACACTGCGCTGAAACTTGTAACTACGCTATTTTCTTTAATTTCAAACATTTTACTGGCTTTATAAACAATACAGAGTGTTTTAAGGTTAAATGCAATATTTACCAGCGTAAATTTCAATAACATCTATGTTTCAAAGCGATGTTAGCGCACATATTCAATTTGAATTTGGCGGCTACAATTGCGGTCAAGATATTAGGATTCTTCAAATGATACTAATTCAAAATAAGCAAAATAACTCCTTTATCTAGTTATTTTATTTGTATACTTACATAAGCGGTTGTAAAAGATTCGGCTTACATCTCCTAGGTACATTTTTTGGACGGTCTTAGTTGGTATGTACACAAACAAATACTATAATAAGGCACAGGACTTTATAAATTATAAACTATAGCCTTCAACAGATATTTTTCGGCTAGTTAGAGTTTCATTTTCGGACGGAAGGGATTTCACA

 

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