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CRIMIC CR01747

Gene CG7272

CG7272

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:15501511..15501528	71D3	FBti0210490	86503

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACAAAACGCATGCAGCATGTGGTCT	AGCTTCAAGCTGAGAACGGTCCTAGA

Images

Construct Design Sequences

sgRNA sequence	ATCGAAGTGTAATTCCCATTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGAGCAAGAAGGTGCCCT	ATTTGGCCAACGCAGTGATG	TCATCCGCGACCGTTACATT	CGGCGTGCAAATCATGGTAC

 

Amplicon 5'

Amplicon 3'

GAAGAGCAAGAAGGTGCCCTACGTCAGCGAGAAAAAGAAGCATGTGCTGAAGGAGGAGGTGTGAGGATGATTGCTAACGACTGTAGTTGGATAAGTTTGAATAAAATTGATTTTTGTAAGGAGACTATGTATGTATATATTAAGAAGCCTAATCTCAATTGAGTTTCTCACTTTCTATCATTGTTCTCAAGTGACTTTCAGTTTGGTCAAGTCAAACCGAAGTTATCAGTGAATTTGCTTAGTTTAAACTATTGTTTGGCTTTAATTCAGTGATACTAGAAGAAAGAAGATGTTGATAAGTTCTAAGGAATACTCTCCTGTTGCTCTAGAGAGAAAACTCATTTGCTTTGCCATGCAGCAACGTTTCGCGGAAATTTGAAATTCAATTCGTATCCGATAAGCCCTCGCGGGCGATAACGTTTGGGGAAAATCGGAGAGACGCGTCCATTTCTAGGGGATTCTCGATTATAAAAGGTTGCCAGCCCCACCGTTGGTGCTCAGTATTTGTTCGTTTGTTGTTACGGCAGCCGCCAGTCGGTCGTTAGTTTTTGTTTTCGTTCATTTGTTTATACTCGCGCGGCACTCGGCCCCCCTCCCGTTCCCACATAACCCGTTTGAAAGGCATCAACTGAGAGTCATTGAAATCATGGAGGCACTGGGGGATCTCTTGGCGCCACACAGCGAACTAATCGCCAAGGTAGCCGGCACCATAACTACCCTGCAATTTCTGTCCGGAGTGGTTCTGATGAACGACATCCGCAAGAAGGGTAGCAGCGACGTCTACCCGGTGGGCCCATTTCTCTTCGGAGTGGTGCTGTAAGTAACCTAATTGGGATTCCGTGATGCTCCACTGCATGCGACATCGAAAAAAAAACTAGTGGGCAGGGGGCAGGAAAAGCGAGTGAAAAGAACAAAAGCCAACGCATCTGTGTGGGAATTGATTAAATTTTAGAACTGTCGCCATGACAGCACAGAATCGAGTCTCTTCAAGGCCGCCAGTGAATCGGTTTTGCACACAATTCAAGAAGATAACAATGGAATCGATTACATCACTGCGTTGGCCAAAT

TCATCCGCGACCGTTACATTTCGAAATCGTTAACCTTGTCGAAAATCCGCCAGCAGCACTTTTGCCGCCTTATCGATGCGAGAACCCTCCAAACACGTAGCTCTTATCTAGAGTGTATAATTTTAGAGCTCCTCTATTACGAATGCTGATCCGGTAGAGTCTGTCCAAATCTGGGAGAGCCGCTAATCGTTTATACGACGGGCGAAAGATACGCGACTCGCCCTCTCCACGTGCTCGGCATAAAGCGGGGCTGCCCAGATTCCAAAGGGGGTGGGCCACCCAGTGGTGGTGATGGGAATATCTTGTCATCTATAAATATAATCTTCTCTAATTATAGCCGTAAGGCTTGTCCATTACTGCATATGAATAATAGTGTTTGCTTAGCAAAACATAATATTTTTTTAACTATAGTTTCCGTAATAATACATTTCTTTAAACAATTCCAAATTCCTGCTGGCTAAAGCACTAAATGTAGATGAACTCTCGAGGAATTTACCTTTTTATTTTTAAATCGTAAACTTTCCACTGTTTGTTTTGAACATCTCAAAAGATCATAAAGTGCCATATGGCAGCAAGTGAATGAGTACCATCAAGTGAGTGTGCCATCACTGCGCCGGGGCTTACTTGGGGGTGGTTTTCCGGAAGGTGTTAACTTGGACTGTGTCAAGTGTAAGGACCCGCAGACATGCAAGCGAAGCAACAGTTGTATGCTAAACGAATTCCACTCCACCCAATGGGCGCTCGAGGTCTCGGTCAAGGGCAGGTTCCTCGGCCAGTTTGCATTTCATTTCGCCCAGCCCGGGTGTATTTCACCATTGTTGTGGGTTTGTTTATAGGTTGATCGATGATAATGCTGATTATTTGTTGAGCGTTGAGCTCCTTATCAAATGCTACGTGTACCATGATTTGCACGCCG

 

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