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CRIMIC CR01749

Gene CG4914

CG4914

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:14676182..14676201	70E7	FBti0210491	86504

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAATTGGTTCGGTGCCTTCAATCGC	ACCACCAGAATCACCCTGACAACTGT

Images

Construct Design Sequences

sgRNA sequence	CCGTACTCATCTGTCTCTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTTTTGGCTAAAGGCGCG	GAGACAGATGAGTACGGCAGA	ACACACGTTCTCGAAAGCCA	TTTTAGTGCCTGCGAGGAGG

 

Amplicon 5'

Amplicon 3'

AACTTTTGGCTAAAGGCGCGGAGCACGAAGCGCGTTTCCGGGCGCTCCTTGTCATTGCAACGATCGTGCTCCCCGAAGGTCACTTTGATCATGAACCACATGAATCCCTTAACGCAGTGCGCCGCCGTCAGCACGTAGCGATCGTTGATTAGTGTGCCGCCGCAGTAGAACCTATTGAAGTAGCTCAGACGCGCCATCCATGGATACTCGCTGACGCCGGTCGTCGTACCGCCCACAATCCTGGACTCGTCATTGCGCTCTCCGCACCTGTCGATAGATAAAGAATATTACAACGTGAATAACTAAAGTCAGTTTCTAATTAATATTTATATTTTCACTGTAATTCAACTGCTATCTCATTAGTAACAACATTTACGAAGTTTATGCCTTTCGCGAGAAGAAAAAAATGGATTGGGTCAAGGGTGACGGGCCTCTCTCCCGATCGGCAAGTTCAGAGTTCAGCAACCCCGACATAAAGAAAGTGTCAAAGTGCCTTGTGGACGTACATAAAATAAATGAGCGATTTTTGCAGATTTAATGGCTGCCAGCTGCGTTACATTAAACAGGTTTTTTTTATTACCCGTGGTAAACAAGAGAACACCGCTTGCAGCCAAAATAAAAATATTCCACCAAGTGGGAAAGGACGCGTAACAAGTTATCTCTCTTCTTTATATCTTTTTATTTATTTTGGCCACATAATCAATCGATCCGGCCGCAACAAGTATTTGGGATTCAGATTTTGTGTATGTTTAATCGTGTCATTGCCCCAAAATGGTGGTCTGGTCGAGAGGCGTCCCGAAACAAAGAAGCAAAATGTGCGACAACGACCCACTTCGGAGACGGCCAAGACAGGCCGAGACGGCCAAACAGGCCAAAACGGCCAAAAGACGAAGAAAACGTCGACGGCATTCCCCTGCCTCCCGCGCAGACAGACATTAATGTGACATGACGCATTGAAGATACGCCTTTTACAGTATCTGCCGTACTCATCTGTCTC

ACACACGTTCTCGAAAGCCAAACAGAGACTGTTATCAAATCGTATGCAAATCAGTTTCTTTTCTTCTTTCACATGCAAAAAAAAAAAGTAAAATTTATATATAGAAATAAAACGGGTTTAGGTATTCATTAACAAAGATTAGAACGGTTGCGACTATCGATTCCAAGTAAATTTATAGCATTCAGAGCTTTTCCAAATATATAAATCGGGGGAATTTATTTGGTAAATAACTATGCAGCTTGGAGCTATAAGAATCTTTAGAGGTCCCTTTTTTGGGACCTAATCTATTCGGAGCCTCCGGAATTGATTTTTATGTCCCTTAAGAACCCACTGCCTTTGTTTTCATTTTTACAGTCGACTCTATTAATACCCTGGGGTGTTTACCTATGCCAAAAGCCAAAGTTGAATTCCCAATTGGTAATGCAATTTGTTTAATGGCCACACAAGAATGCACTCCGAATGCAATTCTACAGAGGGAACTCGGCTTCGAGGTCAAGCCGGGAATTGGCTTTTGGAAACGGGTAACGAATGGGTAGGCGATTCTTTTGGGATTAGAAGAATGTTTTAAAAATGTTTAAGACTTCACCTTTGCTCACTTGAAGGTAGTCAGGTTATTACTCAAAAGATGGTTAAAATTATTCTTAAGGCAATGGAGTCTATTGACAAGTTGAATGGCAACGTGATTTAAAGACTTGTATTTTGAAATAGGCGAAAAGATCAATTCCTGATAAATGTGCTCTTAATATAAGATAAGATAATATAAGATTTGAGCAATAAATAAACTGAAAATAACAGCAATATTAAAGTCATTCGGACCTACGTATGTTTATTTGGTTTCGATACATTTCCCACTATGCTTTCCCATAATTAAAAGAGAACTTGGAACTTAGATGGGATCTAACTATTGTCGGTCTCCTATGATTACTTTGGAGGAGTGTGGCCAGGAATAAATATATCTCCGATTGCGTGACCTCCTCGCAGGCACTAAAA

 

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