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CRIMIC CR01750

Gene l(3)72Dp

CG32155

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:16236999..16234140	72D10	FBti0212965	91293

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTCAGTACCTTCTTCAACTGGAGG	TCAAAGTCCTGGCCACAACTTTGGTG

Images

Construct Design Sequences

sgRNA sequence	CGATAAGGTCGAATAGGGTAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAATCGGTTCTGATCGCAC	GACCTTATCGAGCAGTGGCA	AGGGTGGCATTAAGTATTCCG	GTACGAGGAGCCCAGAATCG

 

Amplicon 5'

Amplicon 3'

GCAATCGGTTCTGATCGCACACACACACGTCCGCCTGTTGCTATCTATCAGCTGTTAAGTAAACAATAACACAGACTTCGTTTGTTTTATAATGTGAATAAAAATCGCGACTGAACAGTTATGATCGATGCAATTAGTTAATTATCCAATAGTGCCCGAATAAAACAGGTTTTTATCCAACTTTCGATGGAAGCCCCAGGAGTAATTCCCCTTCTCATTGAGCACCCTGTGCCACGGTCACAATACTTTGGAAAAAAAATATAATCAATTAATCGCAGTACTATCTGCTGGACTCAAGTCGCCAGGGGTACACCCCCCGAAGGCCCTAGAAATCACACATCGCAAACTCATGCCCACGCACACAGCCTGAGCCATTTGTATAGGTGAGGAAAATCGTCGCACCTTATATAAATTCAAATCGACTGTTGCCTCAGCGGACAGTTCCTATTAATTGGTCACCGAGGTTGGACTCACCTGTGCCCTTGCCAAGACTTCCCGTTGGGAAATACTTCTGGCAGCCGGAAAACTTGCGAATAATTAAAACAACAAGCTAAGAAAGAATTCCCTGAGAAAGCTAGCTTACCGACTTAGCGTGTTTTTAAAGAATTACCTGTGGAATAATCAATAGCTCATAACAAGGTACTTAGGAAACTGAAGATACCTCCAACCGAACCAAAGAAAAACGGATATTTCGGAAGATAAAATACTCGTTTTAAGATCTAACAAAATGCAGAAGTTCTCGATCAGCTTGCTGATCTTCATCGGAGCTCTGTTGGCCGCTTCAGAGGCTGGCATCTCATCGCCCATCATAGGGGTTCTCACACAGGAAGTTTACGTCGATGGTTTGATCTCGAGACATTTTGATAACAAGACCAGCTATATAGCCGCCTCGTATGTGAAGTATCTCGAAGGAGCTGGAGCCCGAGTAGTGCCGATTTGGTAAGTGAGCAGAAAGATTCAAAGATCTTATCGTGACCGGTAATTCTGCCACTGCTCGATAAGGTC

AGGGTGGCATTAAGTATTCCGACACGTGTATTTATAGAACATAAATTCATTAGAGAGTAAATTTGATTTGGAGACCGAAATTTCGTTGTATTGATAAGATTGCCTGAAATCAATTAATAAACAATTTTCATACAAATACCTTTGACAGGATCGGTCGCAATCGCAGCTACTACGACGATCTCATGCGCAAGATAAATGGGTAAGTATAAATATACTTTCTAGGAAGTTCCCGCATCTAAATTTATTGCTACTTCAATAGAGTCTTGTTGCCCGGTGGAGCCACTTGGTTTAATCAGAGTAATGGGTATGCGGATGCGGGCGAACACCTCATCCACTTGGCCATTGAACTAAATGATCAGGGCGTCTTTATGCCGGTGTGGGGCACTTGTCTGGGCATGGAACTATTGGTTTACAAGCTGGCCAACGAAACGGAACACCGCATTAATTGCGAAGCGACCGGCATGGCCGTGCCTATGGAGTTTAAAGAAGGTATAGTTTGTTATATGAATTTATATATTCCGAATGCTGAATCTACATATTTTTATCTAGACTATAAGAAGAGCCGCCTGTTTGCATCCATCACCGATGATGTGGTGGACACCATGGTCAAAGAGAATGTGACCTATCACTGGCATCAGTTCTGCTATACGGAAAAAGACTTCGAGCGGGATCTACTAAACGAAACTTGGCGGGTTATGTCCCTAAATCATGACTGGAATGGCGTTGAGTTTATTTCCACCGTAGAGCACATAAAGTACCCCTTCTATGGAGTACAATTCCATCCGGAGAAACCGCTGTACGAGTTTACAAAGACAAGCATTCCCCATACCGCAGCTGCTGTGTTAAGTGGTCAATTCTTCGCCGATTTCTTTGTGAGTGAGGCGAGGGAGAGTAATCAGAGCTTTTCGAATGCCACGGAGCAGGCCAGGACGCTCATCTACAATTATAAGCCGGAGTATACTTCGATTCTGGGCTCCTCGTAC

 

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