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CRIMIC CR01751

Gene CG32104

CG32104

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:12396818..12396825	69B4	FBti0210492	86505

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGAAGCCTGGAGAGCAGTTCAATGC	AGAGGTTCGAGCACTGCTGGTTGTAT

Images

Construct Design Sequences

sgRNA sequence	GCACTTGACGGTTTCATAAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTACGGCGTATAGAGCTGC	AGCGGTATCACAGCCATCTT	ACCGTCAAGTGCTACATTGC	TAGAGGTTTTGGGGAACGGC

 

Amplicon 5'

Amplicon 3'

CCTACGGCGTATAGAGCTGCGCTCAGAAGCGTCATCTGGCTAAACTAGATATTAAGAATTAGTTTGAATTTATTATATATAGCAGAATGAATATATAGGCACTCACATCTTTGATCTTCGCGACGCGGGAAGCAGCATGCATCCAGCCGCAGCAGCAGTTGTTAAGCGGCTCCACGAGCAGCTTCTGTCCCTCGACATCGCATGCTCCGCTCTCGCCACTGGCCACCAGTGCGACCAGCAGGGCGGCTGCGTGTTGCCGCACCAGCCCGGATCCTCCCACTCCATCGAAAGCGCAGTGATTGCCATGCCAGTCCAGTAGCTGTCGCAGCGCGGGCAGGAACTGCCTATAAATTACACAAAATCATAGTAATTATTACTACTTGGTTTGACTATAAAAATGAGGCGCAGCCTAAAAAGCACTCAGTTCAACTTTAGCAATTTTTGGGGAGAAGCAAAAATGGTTGCAAGGCATTTTAGTTTTTTTTTAGCACTACTCATACTATATGATTTAATTCCTAGTAATCAAGGAGTAAGTATTTGCTGCCACGCAATAATTGGAAAATTTATTTAGTTGTTGTGCCTCTTATTAGGTGGAAATTAATCCTACGATCATAAAGCAGGTGAGAAAGCTGCGAATGCGATGCTTAAATCAGACAGGAGCTTCTGTAGGTAAGTAAGCCTTTTTTTTTTGGTGGAAATTCAAACAAAAATGGTTTATATACCCTACACTACAGATGTGATTGACAAGTCGGTGAAAAATAGAATACTACCTACAGATCCCGAGATCAAGTGTTTTCTCTACTGCATGTTTGATATGTTCGGATTGGTTTGTGATCCTTACAAAAATTGAAATAATTTCTTAATAATAGAAAATCACTTTCCCTATAGATTGATTCACAAAACATAATGCACTTGGAAGCACTGTTGGAGGTTTTACCCGAGGAAATACACAAAACGATTAACGGATTAGTCAGTTCATGTGGAACTCAGAGTATGCAAAGTGTTTTTAACAATTAGAACAAACTCAAATGTAGCTCTTTTCAGAGGGAAAAGATGGCTGTGATACCGCT

ACCGTCAAGTGCTACATTGCTGTAAACGGAAAATTCATATGGGAAGAGATAATAGTGCTACTTGGGTAGCGCTAACCAACCTAAATATATCCCGATCCACGATTCCCAAGAGCAGCAAACAGCGCAGGATGCGCAGTGCCTCGATCTGAATGCGCACCATCTGGACCTACCAAAGAGATAAAAAAAATAAAAAAAATAAAATTACTTTGACAAAAGTGTACGCATACAAACCTTATTGTCTTGAAGGAACAGGTATTGTTGAAGTCGCGTAATGAGGACTCCTTTGGAAATACCCTTAGCTATATCAAGGTGGTGGCAAATCAACACTCGGATTAGTTTTAGAAATAGAACTTGCGGCTGGCCATAGAACTGATTGCTTCCACTCGAAGAGGGTAGAAAATGTTTAAAGAGTGTATCTATAAGATGGCTCTCATTGGCCAACTGACGAGCCACTTTGGTACTTGTACGAGCTATCCTTATAAGTAATTTCAGACAGGACTCCACTGTGCTATTATCTGGTTTCACAGAGACCAAGATGAATCTAAAAACAGGTGCATTAGAAATAGCTGTAGCTCTAACACTTTTCACTGCTTACCGAATCCTTTGCAATATATTTGTTCTTAGCAGGCAGTCCATGAGATCTGTTTCAGCCAGTTGAAAGTCATCCATTGACGTGCGACTTTCCGATTCATCTTCATCCACTTCTGCACGCACAGATTTACTGGTGGTCAGTAGCTTCATGTAACGCTGCAGGAATGCTATGGAGTTGGTATCTGTATCTTCCCCATTGCCATCACTGCCGTTCCCCAAAACCTCTA

 

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