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CRIMIC CR01753

Gene CG7841

CG7841

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:15508252..15505188	71D3	FBti0212967	91295

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCATACACGTGTATCGGTTGTACAG	GTCCAGTCGAACGTACATGCACAGAA

Images

Construct Design Sequences

sgRNA sequence	GCATTGCCGCGAAGTTACGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGAAGTCCTGTAGCGCTGG	CAATGCTCGGCACAGGTTG	TTGGAGCCGTGACGTAACG	GAGTTGTCTGCCGCATGAAC

 

Amplicon 5'

Amplicon 3'

TTGAAGTCCTGTAGCGCTGGACTCGGTGGCTCCACCCAGACAGTGGCTGTCGAGGCAGCGGGCACTGGGCTGTTGGCCATTATGCTTTCCACTGGACTGGCGGCGCTGTTGTTGTTGGCATCCGCTACAGGTTTCTCAACAGGCTTGGCGGATGAGCTCGAGTCGTAGGGATTCTGCGCCACCAGCATGTTCCAGGCTGCCAAAAAGTAACAAAAGTAAACCACATTTATTAAATACTTATAAGTACATCTTTACAATCATATTTCCGACTTACAGTCATTTATGTAACGTATTATTTCCTCATGTTGCGGCGTTATCACCTCATCCTCGATCAGTTTGGTTGGCGTTATCCGCTTGGTGGCGAAGCTCTGTTGAAACACCGGACGCGGTATGCTGTTTTTTTAAAAATCAGGAAATATGCATACAATTTTAATAACAATGTTCTTGAATTTCAATGAAAAGGCACTTATGCAAACCACACAGATTCCACCAAACATGTGGACTACGAAAATAAATCACTTGGAGAACGAGGTGGGGTAACTTGGGTTCGACAGCTTGCTACTCAGTTCGAGGAACGCTAAATGCATTTTAATTCGAGAAAGGCGCTGCAGTTCGTCGATTACATTATTTTGGCTTTTAAATGTTAAAATTACATATCGATGAAAAAATTACAAACTCAATATATACTCATAAGAGCAATGAGTACTGATAAGCTATCTACAGTGGCAGATAGTAAACAGTGCCATCCCATTGACTGACAAGGATTTGTGGTTTATTAATTTAGATAATAGTATTTCGGCAAAGGGTTAGGTAATTTTTATCTTGAAGTTCTAAAAATAAAAGCAATCGCAAGAAATTAAATACAATTGGGAGTTTATAAAGTTAATATTTATATAGAGAACTTTTCAGATTTCAGCTGAAATGGCTATGGGAAATTCTAGAAAACTCGCATCCATAAGATACTTTTAACAGCAAGCGTAATACAGTTGATATAAAAGGCATAAAAATAAACAAGAAATGAGATACACTCTTCAATTACCTATAAGTACTCTACTCTGAAGTTACATGTTTCGATTGCAGTTAGACATTCGGAATAACTCCACACCCTTCCAGGTAAAATAAGTAAATTAATCAGCCTTGTTTTGGCACAACCTGTGCCGAGCATTG

TTGGAGCCGTGACGTAACGGACGTGGAAAAGTCAATGCCTCAATGGCCTCAATGGGCATTTAAATTAAAATGGAGCCGATAACCGACGCCTGACCCACGTGTTTCCAATGGGTCTCCCCTAGCGAGAATAAGACTACTTGCTCGGGAGCAATTAGCATTACTGTGCTAGGTCGCCACGGATTCTTGCGATAAAATGTCAGTGCGATAAGACGAGGGTCTTGAACCCCAGTCCGCGTTTTCCCCCGTCACTTTTCCCGCTTTCCACATTTCACCATGTGCCATCCGTTTGCATAAGCACCCAAGATTTGGGTATGGTTACATATGAGCATGGCATTCTTACTTTTTGTGCTCCTCGGGACTGTTCAGATCGAAACTAGCGTTCTTGCGGCCGCTCTCCTTGATGCTTTCGAATTTTTCAAAATTTGGTGCCAGGCCTGCGGATGGAAAACATGCCATGGAAAGCTATAAAATACGCATTTACTGTGGAAAACGCCGGCAAAACAAAAAGTTAACAGAGCCAGAGAGCAAGAGAGGCAGAGGCAGATATATAGAGAGAGAGAGAGAGAGAAAGGAAAAGAGTATGTTTTTGTGACATAACAGCTGTTCTCTTGAAAGGGTCTATTGTGGCATTATTAACCATTATTACTATCAATATTTTGGACTCACCGCGACGCGTTTTAGCAACAATTTTGCTAGGTCCCTTGTTGATTGTGTACATGGTGGTGGGGAGATGGGGAGTACCGGCTGAAAATATACCTTGTCCTTTCGATTTACGTATTGTTGTTTTCGTTTCTGGCAACGAAATGCCTAGCCGACTGCTATGATTTTGAGTATTTTTCCGCTCTAGGCAATCATATGATCATTGGCAGCCCAGCCTGCCCCACGCCGCCTCCTTTTGGCCTATTGTCATCGTCTCAAAAGGTTACACAATACACTTCACTTAGGGCAGTGATTCCGATTTAGCACTCTTTGATTTTGATGGCTGCCGCACCGCGCTCGTATCAATTGTTTTCGATTAAAATCACCGACAATTACTCCTGGAAACGCGTTTGTAATGTTCTGTGTTCGGCGGACGAGGCTCCAATGCAACTGCCCCATTCAAATCGCATAACGAAATGTTAAGAGAGACACCCGCAAAGAGCCGTATGTTAGGGCGTTCATGCGGCAGACAACTC

 

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