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CRIMIC CR01754

Gene Pgant6

CG2103

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:2783539..2783559	62F4	FBti0210493	86506

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCGTTGTGAATGTCCGAACAGCTG	GTGTCAAGTTCGGATAGCTACATGCC

Images

Construct Design Sequences

sgRNA sequence	TAAGGCAGTGGCTCCCAAAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAACCCCGTTTGGATGGAG	GGGAGCCACTGCCTTATCTC	TGTGACGCTCCAAATCAGTT	GGGCGCATCGTACATTTCAC

 

Amplicon 5'

Amplicon 3'

TCAACCCCGTTTGGATGGAGCTTTGAGTAGAATACAGTCGTAGAGAGGTGAGAACAAACTCCTGATAAGAATTCACCTGCTGATGACGTTTCACCTGATTAAAATTATAAAATCCGGAATTATAAACAAAGTTAGTCGCTGAAGTGTTTCTAAGTTATAGTACATTCATTGACATATGTCTGCGATAAGGTAACTATCCAGCGGATGTTCCTTCAGCGCAGTTAAATATCATTTCTATTATCTTAACTTTCGTATTGGTGAAAATGACTCGCCGCTTATCTTGTATTTTTTGTATGTGCGACCCAAAAAGATACATATGTGCTTACGTTTCTGAATTTTTCTACTTTTCTACTATTCTCTTTCACCCACAGACCACGTTGCAGTGCCGCCAGATAAAGAGACAAGCCAGCAAACATCCGCAGCACCTGAGCTAACAAACCAATATCACCATTACCGCGAGATATACTTCACCGACATTAATGGTTGACGTAGAGACCCGTAGAGCGCACACGTAGCCACGGATATCCGGCAAAGATGCGGCGACCCAATCTCAAGTGGATAGTGAAGGCCTCGCTGCTCTTGTTGATCTCACTGACTCTCTTCGTACTGATAACCAGCTGGATCTCATCTACTCCGTACACCAACAAGCCAGTCCATCATGGCGTGGAGCCCGTGCCCGAAAAGGCTGGCCTTTCCGGCGATGTCAAGGTGAAGGTGCCAGCTATAAAGCAACCAGAACCTCAGAAACCACAGGAACCCGACTTCGAAGAGGATCCTGAGCTGCAGAAGATCGATGAGCCCGAACCGGTGGAGGAAGAAGTCGATAATCCGCATCCGGCGGACGACGAACCGCAACAGCAGCCCCAGGAGGAGCTCCAAATGGCTGCGCCGGCGGACGCTTCGGTGAAGAAGGACTGGCACGACTATACTTTCATGGAAAAGGATGCCAAGCGCGTGGGCCTCGGGGAGGGCGGAAAGGCATCGACGCTCGACGATGAGTCCCAGCGAGACTTGGAGAAGCGAATGTCCCTGGAAAATGGATTTAATGCCCTGCTCTCGGATTCCATATCGGTTAACCGCTCGGTGCCCGACATCCGCCACCCTCTGTGAGTTGTTCATCAAAAAGCACTTAGTTTCGCTAAATATAGGAGTCGAGATAAGGCAGTGGCTCCC

TGTGACGCTCCAAATCAGTTTTATTTTCAAAGTCAGATAAGACCATTTTGCTTGCTTGAAATGCTTATTATTTTATAGTAGGAAGATATTGAATTAATTATATCAGTGCAGTGATATCTGTTAACGAATAATTTATTGCCAATTCCAATTTATTTTAATCTATTAATTCAATTGCAGATGTCGCAAGAAGGAGTACGTAGCGAAGTTGCCCACAGTCAGTGTGATCATCATCTTTTACAACGAATACCTGAGCGTGCTGATGCGCTCAGTTCACAGTCTGATTAATCGCTCGCCGCCGGAGCTGATGAAGGAGATAATCCTGGTGGACGATCACAGTGACCGCGAATATTTGGGAAAGGAGCTGGAGACCTATATAGCGGAACACTTTAAGTGGGTGCGCGTGGTGAGGCTGCCAAGGCGCACGGGACTGATTGGTGCCCGAGCGGCGGGCGCAAGGAATGCCACTGCAGAGGTGCTCATCTTCCTCGATTCCCACGTGGAGGCCAACTACAATTGGTTGCCACCGTTGCTGGAGCCGATTGCCCTGAACAAACGGACGGCGGTGTGTCCCTTTATCGATGTGATTGACCACACCAACTTCCACTACCGGGCGCAGGATGAGGGAGCCCGAGGAGCGTTCGACTGGGAGTTCTTTTACAAGCGCCTGCCATTGCTGCCAGAGGATCTAAAGCATCCGGCCGATCCGTTCAAGAGTCCCATCATGGCTGGCGGTCTATTTGCCATTTCTAGGGAGTTCTTCTGGGAACTGGGCGGCTACGACGAGGGCCTGGACATCTGGGGCGGAGAACAGTATGAGCTAAGCTTCAAGATCTGGATGTGTGGTGGTGAAATGTACGATGCGCCC

 

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