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CRIMIC CR01757

Gene Gr77a

CG32433

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:20739430..20737569	77E4	FBti0212968	91296

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGTACAGTCTGACCAGGGTGTTTG	TTTGGCTGTAGACTCTAGAGGCACCT

Images

Construct Design Sequences

sgRNA sequence	AAACAAACAGTAATCATCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTTGCCGAGAAGATGCCAA	AGGGAAATACCGACGTCGTT	TGTTTCGATCACAGTCGGAG	ATGCACACTCGGAAGCTGAA

 

Amplicon 5'

Amplicon 3'

GTTTGCCGAGAAGATGCCAATTGATTTTGATTTTCGCAAATTATAACGAGACATTTATATGATTTGATAGCTGGCCAGAAATTCGCAAACAATTCCCGGCGACCAGGCAAGGTCATATCGATTGTCATCAGCTGCTGTCGCTGCCCCACCACCCACCGCCCATCACTCGCCACCAACGGCCCACCGCCCACCGCCCACCGACTGACACCACCCAACAACCCACTGAAGAGCAAACAGAGCAAATATGCCATACTCGCATACGCCAAGGAGGTGCTTGCACCAGATGCTAATAATATATGTGCTAATATTTGTTTCCAGTGCAACGACACGTAAGTAAAGTCACCCCTTTCATGGGATTCTGTATGCTATCACTACATACATATGTACATATATATACATATATTTACCCAACCTTAACAAGGGAACAAATTAATTTATATTTATTTATGGCCACTGTCTGGGGAGTTTGTCAGCTATTTGTTTAGGACTTTATCAAACTGTATCAGTATCATAAGGTATTCTAGTATTGCACCCACGATGCAAAAGAGTATCGCATTGTTCAGCTTAAACAGTCCACAGGCGCTGACGGCAAATACATGCTCCACATTCTTCAGATACAGGCCGTAGAAGTGCATCTGGGGAATAAGATACATTATTTAAAGCCTGGCTAATGATAAAAATATGACTTGCACACAAGCTGTTTATCATATGCTAACAAAAAAACCGATAAGCTATAATTTCTACTTTTCCACCGACAATGAGTGGTGAAAAAATTATAGAAATCCAGCTTATTGTATTCATAAACTTGAATGAGTAATACGTAACGACGTCGGTATTTCCCT

TGTTTCGATCACAGTCGGAGTTATTTAGGTATTCCAATCACTTTCGATAACTATTTTATGAAGTGCGATTTACAATCAACACCTGCTGAATATAAATGGCACTTATTAAATAAAACCAAATTGTTCTATACATTGATCTATTAGCATATAATAATGACAACTTGCTGCTACAAAAAGTAGATATTTTTCACAGAGCACCATTTAGAGTGAAGTAACTTAAAAAATAATACACATTTGAACTTACAGTGTGCCTGAGTTGCTTAACAGTGATCCTGGATGGAAAGGCCAATCCTCCGCCTTTGATGATTTCCCTGGTACAGTTGAATCTTCTTTCTGAGTAGTAGGTGGGTAAAATGACCAGCAAAAGGTTGGTGAAGAGCCCCACCAAAAAGGCGATGGCGAAGTAGTTCAACGGAAACGATCTTCCATATTTTCGCAAAATAAACTTGCTGTACTTCATGAAGTATGTGAAGAACAGGTGAATCGTGCACTCCAAGGGCACAAATCCCAAAAAGCAGACGGCCTGAACTGCGCACAACGAGCATATGGACCGATGGAGCTTCAACAATTGATGATGTACATGAAGTAATCTCCACAATTCGAGGGCCAACCACTTTTGTTGGCCTTTGTACAGCTCCTGGCGGTTGGGCCAAAATCCGGCCTTCTGGGAACTTTCAAAAATATGCTCGGCATCCGCCAGAATTTTGGCCAGCAGCAGATTGGAGTGACGAACGAGGTGACCCAGAAATAGTATGGCCATTGTGTACATCCCCAGGGAAAGCTGCAGGATGGCCTGGCATACGGATACGAAAACCAAAGAGCAGGCGTACATGAAGTTCCTTCGCACTTCCGGATCATCCTTGAGTATCTGAATGGGCTGAGCCATGGAGAGCAGATAGGAACTGAGTAGCACCACCACTTCTAGCAGGTGTCCCTTAGTGGCCAGACAATCCATGGAATCCTTCAGCTTCCGAGTGTGCAT

 

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