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CRIMIC CR01759

Gene Vps13D

CG32113

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:12768605..12755049	69E1-69E2	FBti0212969	91297

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACCTGTTCCGATTCCACTTGTTCC	CAGTAACAGCCGACTCTATCACAGGT

Images

Construct Design Sequences

sgRNA sequence	AAGTACAACTGTGTTTATTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAATTCCGCCCAATTGTCGG	GGGGTACAAGTTCATTTTGGG	TTGTACTTCCCATGACGCAG	GACTCTATTGCGCGGTGAGT

 

Amplicon 5'

Amplicon 3'

GAATTCCGCCCAATTGTCGGTGGCACTGCTCTCGGGTACTGTGATATAATTGTTTATCTTTATTCCGCATCGCATAAACAATATATTTTCCCGCAGGCGAAGTTGAGCTCGAGAACATACCCATACGCAAAGATGCCCTCCGCTCGTTCAATTTGCCGGTCGAAGTCACCGCCGGCAGTATCCGTAAAATCAAACTCCAGATTCCGGTCCGCCAGTTCAGGACATCGCCATGGTGCATCTCCATCGAGGGCCTGTTCTGCATCATCTGTCCGAAGAACCTGGACAACTGGGACTACGAGAAGGAGAAGTTGCAGGATTTGGAGTATAAGCTGGCCGTGCTGGACACCGCAGAAGCAGGCTGGCGTTCCGAAAAGGGCAAACAAATGGAGTCTTACTACTTCTCATCCTACAATAACTGGCTGAAGTATGGCACCAACATGGCCACCAACATCATTGACAACATTGAACTCAAGATATCCGATGTGCACTTTCGGTTCGAGGACATCGTGGACACCGGCAAGAGCAAGATATGCACGGGCATCAAGATTGGATCTCTGACCGCCCAGAGCTGCGACTGTGATTGGACCAATGGGTCCTACAAAATGAACAACAACGAAATGAACTACAAGCTGGTGGAGCTCAAAGAGCTCTCGGTCTACTGGGATTTGTTGCACGAGGACATCAAATGTCAGTCGTACTCTAACCAGGAGATTCTCGAAAAAATGCACTCCACCTGCGAACTGAGGTCCCACAATTTCATCATCAAGCCCATTTGTGCAACGGCCAGGTGGAAGCGCGATAAATGCCAGCAGGTGATACGTACCAAAGACAAACCCAGGGTTTCCTGTGAACTCCTGGTTCCCGAGGTGGTCATAGACATATCTAAGGTAACTAAGCCCAACTTGAAGCCACAAATAACACTTAAATGAGACGTAATTTACTGAGGTCCAAAGTTCATCAGAATTAGGTACATATTTCGATTGCTTTTTAAGCGATTATCAAACAATACATTTTATTCGTGAATGAATAATATTGGTTCAATATCAATCACCAACAAATTTGCTTATTTCTATCTAACCAAGCCCCAAAATGAACTTGTACCCC

TTGTACTTCCCATGACGCAGTTGTTATCACCATATTTGATATGCTTATTATTTGTTTTAATAAATTATTTTAATATTTCCAGGTCCAGCGACTGCAAATGTTAGATAAGCTGTCAGAAATCCGTCAGGTTAAGGAAGTTCGACAATACAGGCTAAAGCGACCCACATGCACCGTAGAAAGTAATCCAATCGCATGGTGGAAATACGCCACCATATGCCATGGATTTGACTTTAAGAAAAATGAGGAAAAGTGGTTAATGCTCAAGGAAAATCTTCGTTACATGCTGCTCTACAAATCAATTATATTAAATCCCAACGAAAATCTATCCGCTGCAGATAAGGAGTTTAAAGCCTACATCGAGAGCGATAGAAAGATATCGGATTTAACCATAATGAGAAGGATATGCTTTGAAAAAGTGTTTACGAAAGGTTTCGCCTTTGAATCACAGAACGAGCAGGGCAAGAATATGCTCTTTCATTGGTTTCCAAACTGGATGGGTTGGTATGCGAACAGTCCCAGTACCCCAAATAATGAACAGGATGAATCGCTGAAACATTTGGAAGATGATATTCTCGTTGCGTTGGAGAACTCACTTCAGAACTCTTCCGACTTAAAAAGCGATGCTGTCTTCGGACACTTTTCAATCAAGCTTCTCAAAGGACTGGTAATCCTGCAGACCGAAGATAAATTAAATGATGGCAGGAACAAGTCTATGGAAATGCAATTCAACAACTTCTCCGCCTACCTCCAGTTGAGCCCACAGTTAACTTCTTACACTGTGGGCATATCCTTACAAGAAGTATACCTAATAGACAAAACCAGTAGTGATACGATGCACAATTACTTAATTAAGCCACAAACAGGGAATACAGCCACACCAAATCAACTAGTAAAGAATGCTGCTCTCCAGGAGGATATCCTGTTTCAATTACAATACGAAAATTGCAATCATTTACGCTTTCAGCTGAACATTAAATCAAAAGGTCTAGATCTTATCTATAATGAGGATGCGATCCAGTGGCTTTTAGATTTTCTGGCCGATTCGAATTCCTTTAAATACTCACCGCGCAATAGAGTC

 

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