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CRIMIC CR01760

Gene GAPsec

CG5978

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8623901..8622039	66D8	FBti0212970	91298

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGTGTGTGTGCACTAGTAGGCAAT	ATGTCCAGAACTACGGTATCGGTCGT

Images

Construct Design Sequences

sgRNA sequence	ACTTGCTGCTAAAATCCACACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATGAGACGATGCCACACGT	AGTAAGCCCATCTGGAACTGC	CACGGTATGCATGAATGAAACA	TTACCGCCCAATGCACTCAA

 

Amplicon 5'

Amplicon 3'

AATGAGACGATGCCACACGTTCATCGGTATTCTTTTAACGACTGACCAACCTATGACTTCTTTAACGTTCTGACGTTTATCTAATTGGGACCACGAAATGTTAAAATTAATTCGTTTATTAATGACATACAAATTATAAGTCATGTAAAAAAGTGAAAATGCGTGTACTTATTGGACTTCCTGTAAGGGTGCGTTTCCAAGATTGGGGCTAACTAAAGTATAGTTGATTGCTTGGTTCTTTCAGTTGTTAAACTAACTAGGGGGCTTTGCAAGCATTTGGGCACAAGGATATATTTCTGGAGGAATGCTACGCCAATGATCCGGCATGAGCAATCACAACATTAATGTCAATTGGCGGATAGTTTTGCAAGAGTTTCACATTCGAGGCAAAGTCGTTTTCCAAAATTGCTTCCCTTTGGATTCTGCAAAATTTCATATTAGTTCTTGCTTTATACTAAAGCTAAGTTTACTTACAATATCATTGAACAGCATATTTTTATCAGAAAATCAAATCGCTGTTCGTCCGCAAACACGGAATCCCAAATGCGCAGTACATCTGGCAGTGGAAACTCCTGCGACAGGAGCAGTGTGAGCCACCTGAAGCTGTAATACTGCGGATGCAGCTCTTGGCTCCTGAGTAGCTCGTAAATGCTTAGGTCCTTTGATTTCAGCATATTCGATAGCCTGGCCATCATGAACTTGATGCCGCCTTCTGCGTCGTCTAGTGTTTTAATAAAAAAGTCTCTTATCTCGCTCATCAGGGCGGTAAAACAAAAGAAACAATCAGCCTCAGCGTGGGCGCGATATGTGAGGTCAGGATCGGAGGCCATAACATAGTAAATGGGTCCCACGATTTCGTTCATGCCCTGGACATATCCCTGGCCGGGATTTAGTTTGGCATAGATGAACAAAATGCGTTGCACAACTTCCCAGTGAGCCTCTTGGCCCTCCTCCATGGCGGCATAGTTTTCAACGGAGCGTTTTGTAATCAAATTTATCTATATAGAGCACACATATTCATTAGGTTTGGGGCAGTTCCAGATGGGCTTACT

CACGGTATGCATGAATGAAACAACACATTCTGAGCTTATTGTCTAGCACAATTCCGATCTTGAACTCACCTTTGTCATGCCGAGGCCCTTGCGTTCCACATTCGCCGAACTTAGAACGGCGGGAACCACCCGTTCGTGGAGCCTTCGTCCATGCTCTCCTTTGCTGTGGACCACAATCTCGCAGGGATAATCGGTGGGCTGCTGGAAAAAGGATATGTCCGGGCACAAGCGGCGGACATCCTTGTCGATTTGCAGTAGAAACTCGTTATCATTGAAGAACGTGTTCCACGCGCTTTCGGGTCCCTCGCTCAGCGGATGATCTTGCAGGCCAACGCCCCCCGAATCGACTTTGTCCCCATCTCCTCCGTCTACGCTAGCTCTATTGCTCGAGTGTCCGGGGGGCAGGACTAGCTCCTCGATGAACTGTTTATACAGGGCTCGCTTTTGGGCCAAGGTTGTGGTCCAACTACTGCGCCTAGGCCCCAAATAGCCAAGGAGTAGCTTCCAACTGAGTGCTCGAAAACTCTGCACATCGGGCACACCTGTGGAGTGCTCAGACTTCAAATTTTGAACATTTCAAATGCACTCTTAGCTTTCTACCATTAAATGCCAGTTTCCTGAGTTGTTTCAGGTCAATTAAATCCTGTGGCCCCGCCAAAACATCCTCAAATTCCTTGACTCTGCATTTTGGTTAGAGAAAGTCTTTGTTTACGGAGAAGTTTGGATTAGCTTAGAATAACAGCTTACCGCGCCTTGAAAATAGACATGCTGCGGCTTTATGTGGGCGGGGCTATGCTGAGATCTTGCAGTACCCCTATGTGTAATTAATTAAAGCTTTATCTCAGTTTTATGCTGGGTGAAATCTGCAGGTATAAAACACAACAAAACAATGGGCGTTGCCAGATGGTTCGAGCTCTAATCGATTATTCTCTGCGACTATCGATTTTTGAGTGCATTGGGCGGTAA

 

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