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CRIMIC CR01763

Gene Cds

CG7962

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8127762..8131675	66B7-66B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGACCAGCACCTTGGACTTCTTT	GGTAGTTGCACAGCACATACGAGA

Images

Construct Design Sequences

sgRNA sequence	AGGGTTTAAATACTTTCTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACACTGCATGCTGGGCTAAT	CGCCGAGCAAAGTCCATGAT	GCACTCCGGTCGTATCGAAT	CGAAGGAAAGGAACCGGTGA

 

Amplicon 5'

Amplicon 3'

ACACTGCATGCTGGGCTAATCAGAAATAGAGAAAAGCATTTTAATTCGGACGAAGTCGACATACGGATCACGCAGCACGGGTCACGGCAAACGGCAAACGCGGAGAGCGGAACACGGACGAGAACTGCTCAAGCGGAATCAATAGGAATCAATACATTGTGAATAGATCTGGCGAACATCGAATAATGGCCGAAGTGCGACGCAGGAAGGGCGAGGATGAGCCACTGGAGGACACGGCCATCTCCGGATCTGACGCAGCCAATAAGCGCAACTCCGCCGCCGACTCCTCGGACCATGTAAGTAGCAATGACTTGTATGTGGAGCAGCCGAGCGGCAGCTGTTCCGGACACCAATTTGCATAATTCTTGGAGCATAGAAAAGCCAATGTGTGTATACCAACAAATGTGTGTGGCTATGTGTTTTTGTTATTCTCTGACGCACAAGCAGGTGGGAGAAAGAAGAGAGAGCTCTCTCCTGGAGAAAATCCAATAACAAACACAAAACACACCACTAGCCAGCCAGCCTGCCTGCCACCCAACCACCCACCTGCTGTGTTGCAATATAGCGCCATTATGACCAGCAAATCCAATTAGATGGACTACGCCAATTAGGGAAGCACTGGGCCAAGAACCCAGAGCCGAAACCATTACCATGACCAGACTCCCTTTCTGGCTTGGACTTTTTTTGTTATTGCTGGGTGATAATGCCTCCTTATCAACCAAGCTATTTGGCAACAAAACACGCCACCCAAAAAAACCCGCAATTTTTCTCCCCACTTGGTTCCCCTCTCTATTTTATGTGCCTGTGTATTGGATAAACGAGTGATTCTGATTATGGGTATATAGGCACATACATACACGTATCATGGACTTTGCTCGGCG

GCACTCCGGTCGTATCGAATCTGATAGGAAACCTACTTTGCAGTCCCAGCCAATGCAATCAATAAGTGAATTTTCCCTTTCCCTTGCTCCTCCTCCTGCCAGGTGGACTCCGAGGAAGAGAAGATTCCCGAGGAGAAGTTCGTCGACGAACTGGCCAAGAATCTGCCGCAGGGAACCGACAAAACGCCCGAGATATTGGACTCGGCCCTCAAGGATCTGCCGGATAGGTATGTGAAACAAATCCATATGCGTAGTTGGGATCTTTAAAGATCATGATGAATAGCTAAAGGTCGATTTGGAATTGATGATTCAAAAAATATTTTATTGAAGTGCGAATTTTATAAATCCGACAGAATGAGTATGAGTGCGTTCCAAAAATAATCCATATCCACAGCTGTCTACAGTTGGCGAGCAAATATTAAAAGTAAATATGTACAATTACAAACAATTATTTTTTGCGCATTCCACAGATGGAAGAACTGGGTGATTCGCGGCATCTTCACATGGATTATGATCTGCGGCTTCGCATTGATCATCTATGGTGGACCGCTGGCCTTGATGATCACGGTAGGTTTAATGGAATTCGCTTAAACGAGTCTTCAAATAATCGTTGTCCTACTTTCTCTTTGCAGACGTTACTGGTGCAGGTGAAGTGCTTCCAGGAGATCATCTCGATTGGCTACCAGGTTTATCGAATTCATGGCTTGCCCTGGTTCCGAAGCCTTTCCTGGTATTTCCTGCTTACCTCAAATTACTTCTTTTACGGAGAGAATCTGGTAGACTACTTTGGCGTTGTAATTAATCGGGTGGTCAGTAAATAATACAATAACCTGATTATATCGCCTGGTAAATATGCAATTTCATCTACAGGAATATCTGAAGTTCCTGGTGACCTATCACCGGTTCCTTTCCTTCG

 

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