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CRIMIC CR01766

Gene trc

CG8637

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:19834304..19830864	76D1	FBti0212971	91299

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ATGGCCAGATTAGCAAAGGCAACTGG	AAAGACCCAGTCCTTCGCAATCTCAC

Images

Construct Design Sequences

sgRNA sequence	ATGATTGGCAAAGGCGCTTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGTAGCCAGTCTGCAGAAA	GCGCCTTTGCCAATCATATG	GCATTTGCCGTTGTATTGGT	CTGGATCGCAGCCAACTTTG

 

Amplicon 5'

Amplicon 3'

CCGTAGCCAGTCTGCAGAAATACTTCGGGTGCAATATAGTCCGGCGTTCCCACGGTGCTGTAGGCGAGGGCGCGTCGATTTCGCTTCCACGACTCGGCACGTCGCTTGGAGTCCATCGGACTGCAGGACAAGCTAGTTTATTTAATAAATACAAGCTTAGATTCTTTCACATCTTACCTGGCGCACGTGCCTATAAAATCGGATGGTTTCGCCTGCGACAAGTCCCGATAAAAGTCTGTTCGATGCGACTTCTTTAAGCCAGTGCACAGTCCGAAGTCGGAGAGCTAAAATAATAATAGAGTTTTTAGTGGGCTCATCTCAATTATCTGGATTCCACTTACCTTCAGATGCCCTCGCGCGTCCAGCAGCAAGTTATCGGGCTTGATATCCCTGTGTATGAAACCGAGTTTGTGAATAGAATCGATCGCCAATGCCGTCTCACTGATATAGAACTGTGTGCCCTCCTCGGATAGCGTGTCCTTCTTCATCAAAAGCGTCATCATATCACCACCAGGCAAGAACTCCATTATCAAATATAAATTGACGGGATCCTGTGGAAAGATATTTCAAAAACATTCTCACATTATTACTGAGTAAGAAAATACTTATATATGAATGCAATTGTATTTACATTCATTGTACAATAATTGTAATATGCAAAAATATCTTATTAAACAAATATTTAACGTCAGAGAAATGGCGATTCAGTGTAGGCTGGTTCGCCTGTGTCAAGAACTTTCACCGAGAAGAAGTTGACAAAAGAGAATCGAGAAAAAAACAACAAAGGCGGCCCACGCAGAGAATGAGAGAGGAAGCGAGAGAGCAAGAGAGACGTGACCATATGATTGGCAAAGGCGC

GCATTTGCCGTTGTATTGGTTTATTGATTTTTTTGTCCGTGTTTTAACACAATTCTTTTGTTATGTGCGCTGCCGCCCGCTCCTTCACCCCATCACCCTTTCTCCCACCCACCCTATATCATTGTTTTTGTTGTAGCTGGCGCTCGTCACTCACCTGGAAACTGTAGTACATCTTCACCACCCACTGATGATCGGCCTCGACCAGGACATCGCGCTCGGCTCGTACGTGTGCCACCTGCTCCTTTTCCAGCATGTCCGCTTTGCGCAGCACCTTCATGGCGTACACATGTCCCGTGTCCTTTTTCTGCACCAAACGCACTTCACCGAACGCGCCGCGTCCGATGACTTTGAGGGCCTCAAAGTCCTCCACACCGAGGCGCAATCGCTTCAGCCGGAGATACTCCGTCTCCTTCTGGGCATGCTGCAGACGCTTCTCCTGGCGCTGCGCCTCCGACAAGCTCTCGTCCTTCAGCTGAGCCTCCAGCTTTGCGAGGCGCTGCTTTCGCTCGCCATACTGCGTCACCAGGTTGCTGTAGTAGTTCTCCAACGTCACCTTGGCCTTGGTGGCCTTGTCCAGTGTGTGGTCGCTGAATCTGATCGAGGCACCGTCCGCGTCCTGCGTTCTGCTGCTCATCATCTAAGCTGGGGTTTTTGTCCTGGTCACCTCGGTCCTATTTGGGGCTTGTGTGTGTGCTCCCTGCGATGTCGGTGCGTGTGCGTGTGTGTGGTGAGGAGTTATGCACTTGCCAATGCACACACACTGCTTTCTTCGCCCGTTTCTTGCCTTTTTCCTCGGTAATTTGTTGAAATTCCTCCCTCTGCTGCTTCTTCTTCAAGAGCCATTCGCATTTTGCATCCCCAACAAAGCAAAGTTGGCTGCGATCCAG

 

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