CR01767

CRIMIC CR01767

Gene enc

CG10847

Information

Donor Location Cytology FBti Stock number
pM37-p03L:3842326..381867963F1-63F3 FBti0212972 91300

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CGAGTGAAAACAACAGCGTCAGAG GCGTCAGATGTTTGCCCTTCATGTTG

Images

Construct Design Sequences

sgRNA sequence ATGGCTTATGACGGGGGCGTGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CTCATCGGGAGTGGGTTCAG CATAAGCCATCACCACCCCC GGGGATTATGGTTCTGCGAA ACCGGGAAGTATACTCCGCT
 
Amplicon 5' Amplicon 3'
CTCATCGGGAGTGGGTTCAGGATCGGGACCGGGACCGGGATCGGGAGCAGGAGTATCAGGACCAGTAGCCGCAGGCACAGCCACTGCCGTAGCAACAGGATCAACAGTCACAGCAGCAACATCCGTCGCGGCAACAACCTCCACTTCCGTTGCCACAATCTCAACCAGCTGCAGCAGCAGCAGCATCAACAACATCAACAACAACTGCGGCGAGGAGTGCCAGTCGGCGGCCGGATCCTCCAACTTGGGTCGACAGAACAGCTTCGGCAATAGACGAGTAAGTATTGCCGAAGAAACTCGAGGTTTTGATCTATAGCATCTTAAGGATATATAAAGGGATATAATCTGTATAGATAATAATTAATTTCGAAAAGGATTCCTTGTGCATTAAAGTTCTTAAGATACATAAACTGTAGCTATCGTAAGCATAACCATTGTTTAAGAATATGCATTACATAGGAAACCATTTAGAATGCCTTATATGCCATGTTAAATAGGTTACTACTATATGGTTCAAAAAGGCGAATGCAGGGATTATAGAACAGCTCTATATAGGTCAGATCTGGTCAGGTGTCGCGTTGCAGTTGGGTTCTCCTCTTCCCACTCAACATCCTTTCGGCCCTGCTCTCGATTTTCGCAGCTCTCTCTTGCAGTGCCCGTGATATCTCTTCGTGTCTCTCGCAGGATGCACACACATAAGTGCAGCCATGAACTGAACTCTCGAGCTAAGGATACACGCCCAAAAAACGTCTAGTAAACTGGGTCAGGTCAGTGGCTGTCCAAGAGGGGGTGGTGATGGCTTATG GGGGATTATGGTTCTGCGAATAGCATTTGCTGGAAATTCCCCAATCGACCCCGAACGGCTAAAGTGTTGCAATTGGTAATCGTGTTTCTGATTGCTCCTGCAGCAGTTCGTCGCTGGCACACAATCGTAGACATTTCCAAACGCTTTTTGCCAGCAGAGTTCAAGGTTTTTGTGTGAGTTGGTGGGTGGTTGGTCGGTCGCTCGGTCGGTTAGTTGGTTGGTTGGTTTGACGTTCCGTCGGCCGGTTGCCTTGGTTAACTGCTCATGCAGGATTTATCGTCAATCATTGTTTATGTGCTGGGCGATAGAGTGGTTTTCATGGCCCCCCTCATTTCACCCACTCCCCAAATTATGGAGCACTCACTGGCAAATGCTGCTGCTGCGAGGCTCCAAGGTCGATTTATTGATTTTTCCCACAACGAAAATTAAATGGGTGTACCAGATTCCTGGAAATCGCTTTGAATGCCCGTTTCAAGAACTGGAAAATTAGCTGCGCGCCTGAAGGTTAGGGACCAAAGTTTTTCATTCAAACAATCTGTCAGTTTCCACAAACCTGGCTTGCTTTTAAGTAGTAAGTGTGGAAAAATTGTAAGAAATTTCCGGAACTCTTGTGGTGCGGAAGATGGTTAAAAACCATCTAAGTTAAGGTTAAGTCCCTAGTTCCTTCTTATTGAAACATGAAGTGCTAACATAGCCTGTTGGCCACGTCAAGTGACTTCAACAGCTGATTAATGAGGTCAGTTAGTTTTCGTCCAGCGGAAAATCAATGGGGGAAAACAAATAGCAAGGGAACGTAATTAATGTGCCTGATGACTAAACTTGAACGATCGGCCGACAGACTGAACGCCTGACTGCGCTTATGCTTTGGTTTTTATCCTGCCCTTCAACGCGCGAAAAATGTCAAAAGGCAATCCTGGGCGGACTCCATGAGGTATACATATCGTGTATAATATTAGCCTAGCACGGCAACCAAGCGGAGTATACTTCCCGGT
 

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