CR01771

CRIMIC CR01771

Gene ArfGAP3

CG6838

Information

Donor Location Cytology FBti Stock number
pM37-p03L:22714313..2271757779F6FBti0216167 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GTTTCTGCACTTAGCGTCCTCATACG AATAGTCCGACGAAGATATGGCTCGC

Images

Construct Design Sequences

sgRNA sequence CAAAGCAATCGTCAGTTAGTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTGGGTTTGCGATGAACCAC ACTGACGATTGCTTTGCTTC AGAGAAAAGTACATGCGCATGA GGCATCTCCGAGAACCTGTG
 
Amplicon 5' Amplicon 3'
GTGGGTTTGCGATGAACCACTATTCTTCTTAACGGGCGCCGACGTTTTGGGTTTGTCATAGTCATTGCTGCGAGAAAACATGGACGTGATATTGCTGTGGCTACCCCCAATAGGCTCAATTGTCTCGTAGCCCAGCGCATCAAGTTCAAACTTATCAAGCTTGCTTGTTCCGCCAACTGAGCTCTCCCGCTTTTCGCTAGATCCTCCTCCTCCTCCACCGCTGCTACTATTTCCGTATAGGGAAAAGTCAGTGAAAAAATTCTCGCTTTCCAATAAGGAGAGTTTTGCTTTAGGGGCGGCCGACTGCTGAATAGTCTCCATATCGCCGAGAGCGGAGTGGGCCATGTCGGAGCCTCGCAGGTTGAAGCCCATGCCCAGCCGCTCCATCTGTTTAGCCTTAGCAGGGTCCATGGTCTTAAGCTTGGCCTCCTCGCGGGTCTTCTGCATGGAGAGTTCTTGGTATGCCAATCGCATGCTGGCCACCGTCTCTAGTTCGTCCTCGGCCGTCTGTGGCTTGACCACAGGTGCTGCTGCAGCTGATGTCTTCATCTCGTTAGCCGCGTTGGCACGGGCCTCGATGTCTGCGAAGTTAGTCTTAACTTTCGTGGCTCCAAGACCGCCCACTTTGCGAGCACCCAGCTGAAAGATAGGAAAGTCGATTAGCCAGTGCTTCACACTGCTGGCGTTGACACCTACTCCACCCTTCTTCGGCTGCACCTTTCGCGCGCCTATGGACGACGGCACTGCTGCCGGCACCACGGAATTAAGCAGATTCACGCTGGGGGCGCCACCTTGTTGCGTTTCAACGCTGATGACCGGAGCAACGGTGGGTGGGTTTGGATCCTGCTGAAACAATATAAGTGTGAGGGATCAGTGAACGTCTACAAACGAAAAATCGAAAGATGGTGCGGGTCCCCTGCGTGTAAGTGGCGCCATACCTTACTGACATTGTTGTTCTGGACATTGAAGTCCACCTCATTGTCACACTGGGCAAAGAAGTCCTCCTCCCTAGCGGCTTCGTTGCCCTCGCTCTTGTCCGTCTGTTCCAAATGCAGCTGGTGGGTGCAAAGTATACAACATGGAGGAGGAACAGAGTCATGGGAAGGAAAGGAGCAGGCGGAGCAGTGAAGCAAAGCAATCGTCAGT AGAGAAAAGTACATGCGCATGAACAAAACGTTTGATTTGAACTCACCTTTGTGCCGTGCGTCTTCATGGCCTGCTGGGCCTGGGCACACAGCTTGTCCCTATAAAGCTGGGCCGCCCGCGAGTTGTACTTGACCTGGGCGTCGGTGGTGCTGCAGTTGTGGGCGCGGAAGAATTGCGCGGCGTTCGCGTTGCCGCCCAGTTGCATCTGGCGGAGCTGGAGCCAGGTCCAGTTGGTGTCCAGGTTGGTGCTGCGCACGAAGGTCAGATGCACGCCCAAGTTGCGGTGCACGGCAGAACAGTCGATGCAAATGAAGATGCCATAGGTAACGGAGGACCAGGTGGGAGCCTTGGCAGCGCAGTCGAAGCAGGACTGCGAATGAGGAAAGGAGAGCAGGGAGCCCCATTAGTCAGAACCCCAAGGGCGAACTGGCGAAAGTAGGCCACTCACTTTGTTAGCTGGCTGGGCGCGCAGGCGACTGAAGACCGACTCGATCTCCTGCTTGGAGGGTCCTGCTGCTGGACTGGCCATGTCGGATGGTTCGAACTGAAATATCTCAACTGAAACACGAGTAATGGTCGACGTTGGTAGACGGCCTTCGAGGCGCTTTCTGGCGCTCTCCGCGACTTCGATTTCCGCTGGCCGAGGGAAGCCCTAGATACCAAACACAAACCTTGCCAAATTGCTCGACTTTTCAGAACGTACGTGGACGTGTTTTCCTTTCGTCGTACTCGACGGAACGAACCACTGCGTGCTGTTAGTTCGCTCAGTGCTGTTATGCGCACTAGTGCTGGACAGCGCACAGGTTCTCGGAGATGCC
 
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