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CRIMIC CR01772

Gene CG3408

CG3408

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:9506818..9509583	67B10-67B11	FBti0216168	92655

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCGAGGATGTGTGTGAGAAGCT	CTAAAGTGCTTCTCATCCGACAGC

Images

Construct Design Sequences

sgRNA sequence	CAAAGCACTTACGTATTTCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCAATGCATCCGAGATTT	CGGTCATGTTTTGCCACTGG	CGTAAGTGCTTTGAAAGTCCCT	ATCGATACCCGGTCAGAGCT

 

Amplicon 5'

Amplicon 3'

GCGCAATGCATCCGAGATTTTTTTTTAGCGATTTATTCCATTTGAAGCCCAGCGCTCTAAGGCTTCAAATAAATGAACCGTCAGTGTTCCTCCGGTGGTGTTCGGAATTCCGAGATCCAGTCGGTAACACGCAGCCGAAAGATCTCCGTTTTGGTCAGGATCCAATCGCGATACTGGTGTGGTATATACTCCACCAGCTTGTCGGCGATGTCCGGATTCTTGAACATGATCATGTAAATGATTAACAAGTTGATGATAAAGAGAACCAGAAAGCTGACCAGCGACGATAGGGCAGCTCTCGACAGTAGATTCAACCAGGAATCGCCTTTCGAGTTCTTTTTCTTTTGATTGTTCTTTTGAGTTGATTTGGATCCCAGTGCGATTGCTCCTGATCCTCCTGATCCCACTGCATTTACTGGTTTGATTGTCAGCTCATTGTCTCCCCCACCGGCGAGTTTTTTAGCTTTGGCTTTCTTATTATTCGGTTTCGTCGACTTCTTTCCACCAGCTGCGCCGCCATTCGTTTGAACGGCGTTGGCACTGGAATCCCCGGCTCCGGCCATTTTCTGGCTCTCTTGGGCGGCCAAACGTTCGCGTTCAATGAATTCCGGCTTGTAGTTGGCGCAGATGCTGACCTGGAAAATGGAGAAAAACCGGAAAAATGTTAGATGGAGCCCGACACTGTGTCACTCGAGGGCCACTCAAGTCGAGGGCCACTTACTGTATTCTTGGCCGCCTGCTGGCAGTCCTTTTGCGTGGAGCAGCAGCCCACAATCTTCTCCCAGGCGGGCGTCAGTGGATTCCCCTTCAGGTCTAAGTACCGCAAACGACGCAGCTGACCGAAGCTAATGGGCAGGTGCTCCAAGCAATTGTTGTACAAGTCCAAGTGGCGCAACTGCTCCAGCTGGCCAAAGTCCTCGGGCAGGAATTTTATTTGGTTCTTGCTTAGGTCCAGGCGCACCAAGCGTGTGAGAATCGAAAAGTTTTTCTAAGGCAAGATGGGGATGTCCGAAAAAGTGGCATTTAGGTCGTGTGCTAGGTGGCTAGATGTGGTGCGGCTACTTACTCCCAAGTTGACCAGACGATTGCTGGAGAGATCCAAAACGGTGACGCGCTTGAACGAAGCCTGCAAAGCACAAGGACAAACCGAACTGAGAACCAGTGGCAAAACATGACCG

CGTAAGTGCTTTGAAAGTCCCTTTCTTTCAATTTACACCCCTTAGTTATTGACAAAATTTGGGGTATTCTGTTGATCATTTAATCTCTGTTATAGGTCATTTATTATTAGTATGTTCTTTTGTTTCACTAAGTTTTGTTTCTTTTTTGTAATCTAAGCTGGATATTGCTTATTTTCCAGTTATAAGGAAAAAACATCAAATATATATCTATTATTTTTTATATTCTTTTTTAATAATTAGTACTCGGTATCTGGTGGTCGCGACACTCTACACTTAAGCTACCATACTTGTTTTTCTTTTTTGGCAACCATCTGAGTTTTTGAGGGGTGGCAAAGACCAAAAATCAACCCCCTCGAGCGAATTCAACTGCATTCGCCGGCACACAAGGGTTAAGGGGTGTGTGGAAGTCGACGTCAGCGTTAATTGCAAGCCAGCCGGCAAAGCAACAACAACAACAACTACAACAAGAGTATGCGGATCTCTCTTTATCCATGCCAAACCGCACCCCCCACCACACGGCACTTGTGCAAGTGAGACAGACAGTGACTAGACATTCGCCAGACAACAAACGTGGACCCAAGAGGAAGCGAGAGGGGTGATGGGAGCTGATGCTAATTACTTGTATTTCAGCTTGTACTTACGATCTCCCGCACCGGTATTTCGCTCAGTTCGGAGAGACTCAAGTCGCAGGTTTCGTCCACCACCCGCTCCTTGACGTTCACCTTCACCTTCGCCTTGTCACCGGGTTTCGGCATTTCGGCGACACTTGCTTATCACACTATTTATACGCGATTTATTCCGGCTGCTGTTCTTCCTCGTCACTTGGAGTTTGTGTTTACCCGAATCTCAAGTGATTTTCAAACGTTCGTTTTGTGAGAATGGCAATTTTGCTGGCGAAGCACACGAAAGCTCGGGAAATAACGAGTACTAAGTTGATAACGGCAGCATTTCGTTAGATTACTTCTCTGGGCACACTGGAGCCTTATCTGGAATTGCAGCTCTGACCGGGTATCGAT

 

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