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CRIMIC CR01775

Gene Sfxn2

CG6812

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:19066151..19063165	75F6-75F6	FBti0212974	91302

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACAGCAAGTACTTGTGGATGCACA	CCTGCTCAGCAGCTCAGTTTAGTTAG

Images

Construct Design Sequences

sgRNA sequence	CGATATCAAGACCAATTGCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATCTGCCCAAAAGCGCAAG	GGTCTTGATATCGTCGTGCCT	CGGAATGCCAAATCTTCGAGC	TCGAAGGTGCGCATAATCGA

 

Amplicon 5'

Amplicon 3'

AATCTGCCCAAAAGCGCAAGCGGCCAAAACAACAACAATAACAAAAGCAAGCCGCCATAAACCGGCAACAGCTGAAAGTTACTGCTTACGTAAGCCGCTCGCAATTACAAATTACAAGCAGTTAGGCAATTGGATCAGCAGCCAAAATGTCGCAGGTATCAAGTAAGAATCAAATCAAAACCACCACTTTATATGGGCTCCCACGAATATCGAATATATTGAGCATTCAAGTGAATACATACACATAGAGGAATCAAAGCAAATAAGTTTTTGATGAATCATTTCTTATGCATATTTTGTGAAAATAAATAACATAAAAGGATATTCATTTCACAGCATCTTCATTATCTAATCAGTTTTGTTGTGTTTTTTTTTCGAAAGCTTTTCAAAAACATAAGACTGATTATGACATTTACTCAACACTTTCTAATATCAATTTATTGTATAAATGAATCTATTCATTTGTTAAAAAAGTTTTAAGAATTAATAAATCTATAGCCATAATTTTTTATTATTTCCATTTTGATTCTCAAAATTCTGTAAACTACATATTTTAAAAATTAAAAGTCAGCTAGAGCTTGTATGTAATATTTCAATTAATACACTTTAATATATAACGTGGCAGCCAAAATAAATGTACTTTCATCAAATTTTAAACAATCTTCAAATAGTAGCAGTATCTAAAATAATGTACTTAATAAATTTGATATTTGAGAGAGCCCAGAGATACGATCATTCTATGCCCACTGACCCAACCAATCCCTAACCCTAGCCCTCATCGATGTGGACAAGCCACTCTTCGATCTCAGCACCTTCGCTGGGCGCTTCCAGTACTTCGCCTGGATGACGGACCCCCGCACCGTAGTCGTATCCAGCGATCGCCTGCTGGAAGCCAAAGCAATGGTTGAACGCTACCGGAAAGGTGACCAATCCCCGCCGCTGAAGCCGGAGGAGGTGCACTACAACATGAAGCTGTACAACTCAGCCTTTCATCCGGATACCGGCGAGCTGCAGAACTTTTGCGGCCGCATGAGTTTTCAGGCAAGTCTCGAGATGGGTTCGGCACGTGGTCGCTGAAAATAGAAGCGTCTGGCGTGGACGCCTTGTGAATGGGTTGCCAAAAACACAAGGAAGGCACGACGATATCAAGACC

CGGAATGCCAAATCTTCGAGCATTTTGTTGGTTTTAGCATCAAAGAACCTGTTCCCTTAAGGGCTTATCTAAATCGATGCTTACATATTTAAGCTTTACTAATTGCTTTGTGTATTTGGTCTATCTTCAGGTACCCGGAGGCATGTTGATCACCGGTGGCATGCTGGCCTTCTATCGCACCGTGCCCGCCGTGGTGCTCTGGCAGTTCATCAACCAGTCCTTCAATGCGGTTGTTAACTACACGAATCGCAATGCCAACTCGCCAACTAGCGTTACGCAGCTGGGCGTGGCCTATGTATCCGCCACGACATCGGCTCTGGTAGCTGCTATTGGATGCAAGAACTATTGGTCAAAGAAAGCGACTCCGCTGTTCCAAAGATTTGTGCCCTTCGCAGCTGTGGCGGCAGCCAACTTCGTGAACATTCCGCTGATGCGACAAAATGAGATAATCAATGGCATAGAGGTGAAGAACGACGATGGAGTGGTTGTGGGCCAGAGTAGATTGGCAGCCATCAAAGGAATCGGTGAGGTGGTTGTGTCCAGGATTGCGATGGCGGCGCCCGGAATGCTGGTGCTGCCATTGATAATGGAGAGGCTGGAGAAACTGCCTGCCTACAGGCGCATCAAGTGGATTAATGCTCCATTCCAGACCCTTTTGGTTGGGTGCTTGTAAGTAACTTTATCATCTACGACCACTTGTTCCTTTAACTGATCTTTTTATTCTTTGATGTTTAGCCTTTGCTTCATGGTGCCCACTGCCTGCGCCCTGTTCCCACAGCAGTGTTCCTTGGACACTTCGATTATGCGCACCTTCGA

 

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