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CRIMIC CR01777

Gene Ent3

CG11010

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:12857428..12853646	69E6	FBti0212975	91303

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAAGGAATGGCAAGCAGATGTCTCT	GATCTGTGGTCATCTCGTCGCTGATA

Images

Construct Design Sequences

sgRNA sequence	CGTGGGTAATACAAGATCGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTCACGCCCACTAAGTTGG	CCACGTAACAATTCACATGCA	GATGGAGGTTGCACTTTCGC	CTAGTCAGCGGCAGAGGATG

 

Amplicon 5'

Amplicon 3'

ACTCACGCCCACTAAGTTGGCCAAAGTGGAAAATAATGCCAAGGTCCAAGAACCACAGGGAGTAATTAAATAGGATTTGCCAGCGGAAATCGTCGCATAAAATAAGAGTACAACATCATGGATGTTGGTGGCCAGGAAACCACCTATGAGCCGCTGGACGGACGTGGTTCCGGGCGTTCGAGGAGCAGTCATTCCACAACCGGATCCGGCCTGAGGCGTCCAGGCAGCATGGACAGTCCGGAGTTCGATAACCGTGCACCCAAGGATCAGAGGCACTCCGTCTACCTGGCCCTACTGGCAGCGGGCATTGGTTTTGTGTTGCCCTACAATAGGTGAGCAGTACATATTATTAATTTTATTATTCATTAATATATACATTTTGTGTGCGTTTTTTAAACATTCTTTATAGTTTTAATATCCATCTTCCTAAAATACATGACTAAAAAGAAAGATTTTTTTTACAAAGCATTATTGTTTTTAGCTATCTTAAATCTTATTTAATCATCTTACTGCCTTGCAGTTTCATTATTGCGGCGGACTACTGGCAGGCACGATTCCCGGGTCGCCCGGTAGCCCTGGACATGTCGATGACATACATCTTCGTTGCCTTCGGCACCGTTTTGATGAACAACATTGTGCTATCGGTGGCACCGTTCCAGTCGCGAGTGCTCTTTGGCTACATGATATCCTTCACCACACTGATCTTTGTCGCGGTGTGCGAGGTGGCTTGGCATATGTTCGCCACAAATACCGCCTATTTGGTGAATATGTCAGCTGTTGCCCTGACCGCCATCGGATGTACTGTGCAGCAGTCGAGCTTCTATGGATTCGCCTCCATGCTGCCCAAGCAGTATACCCAGGCGGTGATGGCCGGCGAGAGTATCGCCGGTTTCCTGGTGTCTTCCAATCGGGTCGTTACCAAATTGCTGATTAACAACGATCGCGTGTCCACGGTGATCTTCTTTTTGACCTCAACGCTCTACATACTCTTCAGCTACCTGTTGCATGTGGCCACCATTAACTCGCCGTTTGTGCGGTTCCATGTGGAGGCCTGCTCCAAGATCGTTTTGCGGCCGGATGAGGTGAGTGTGCTAACCAAAACGTGTATCTTCCTTAAGGATAAAAGGTTGCATGTGAATTGTTACGTGG

GATGGAGGTTGCACTTTCGCAAAAACAAAAAAAAAACAACTTAAAATTTAATACAACTTGAAAATTAAAATACGCATTTCTTTGTGCAATTTAGCAGGAGATTGATGGAGTTCCAAGTAGTACTCGATATGGTGTGCTCTCGATGGACGGTACCCACACCACGACCACATCGGTTGGACCTCCAGGCACTGGAAATACCCTTAGTTTTAGCAATCCCGTCTACGAGCTATCCAACCCGACTGCCGGCGAGAGTAGCATTGAAGCCCTCGGACAGCTGCCCGAACTTCCGGCCACGCCCCCAGCTCAGGAGCCTACAACGCCCACGACGGTGGCCTTCAAGGTGCAATCTCATTGTTACGCATATCAAGTATGTCAATTGACTAAACCCAATATTCTAGGTGGAACACGTTATCACGCCACGTCGTTGCCGGCCCAGCAAGTTGGGTGACATCCGCGAGGGATTCGTGACCCGATGGCGAGTGGCCCAGGTGATCTACCCGCACATGGTGTGCATCGCCTTGGCCTATTGTGTGACCCTCTCGCTGTATCCGGGTATCGAGGTTGAGGTGCAGTCATGTGCCCTTCGCTCCTGGATGCCCGTGCTGTTGATGTTCTGCTTTAATACGTCGGATGTGGTTGGCAAGATACTGGCCGCCAGTCCGTATCCATGGTCGCGTCGCCAACTGATCCTGCTGTCGGGACTAAGGATTGTGCTGGTGCCGCTCCTGCTCCTCTGCTGTGCTCCCCGCCAGCGTCCCGTGATATCCGGCGAGACGGCGCCGTTTGTCTTCACCATCGCTCTGGGCATCACGAATGGATTGGCAGGCAGCCTGCCCATGATGTTGGCACCGGCAAAGGTGCCGGGAACACTCAAGGAGGTCACTGGCAATATAATGACCCTGTCGTATAATGTTGGTCTGACGGTGGGCTCTCTGATTGGATACGTTTTCGAGAGTATGCTGGGACCGCAGCTGGTGAATCCGTGTCCCACATATCCCTATGTGCCCGCCTCGATGCTGGAACAGTTCCATGGTCACGGGCATGGACATGGCCATCTACCACATCCTCTGCCGCTGACTAG

 

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