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CRIMIC CR01779

Gene CG12003

CG12003

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:1567802..1565392	62A5	FBti0212976	91304

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTATGCACTATCAGCGGATCACAC	AGGGCTTTGGGTAGTCGTAGCCATTT

Images

Construct Design Sequences

sgRNA sequence	CTTACTGTTCGACTTCTGATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGATACCCTGTAGGCGCC	GAAGTCGAACAGTAAGTTGCTTT	TCGGGTCTTCACTTGAACCA	TCTTTTCACCCTCGGGCTTC

 

Amplicon 5'

Amplicon 3'

GATGATACCCTGTAGGCGCCTGGCAATTCTGTATTTCCTACATTACTACTTTCCAACTTCCTTGGGGGAAAAGCTGTGCTCTGCTCCTGATTAAATCTCGAGTTGATCGCTCGGTTGCACACACATTAGACCCACTATTTCATGGATTCGCATGGCAGTTTCTCATGACAATCTTTGAGGCTGGCCAAGACAAGCGTGCGTTTTGGTATTGCGTAAGACGACCAGTTTGCAGCCAGCCAGCTAGGAGGAGCTACTCTCTCCCTAGCTTTTCGCCAGAATAAAGGTCTAATTGAGATGTCTCACTTACAGTAGCAGCTGGTGACTTTCGCACATGATCTTACCAAGGACATGAGGCATTGTGTAGAAAAAAGAAAACTATCTCTACTTAAGCTGTGTGGTTAACAGGCAGCCACCCACTGGAGCTACCACAGATCCACATGCTCCACGATCATTAGCCAGATTCAATTTCCTCATTAGTCGCACTCAATTATGTGCCGACGAGACGGCTCCGCAGCGTCCACTGGAGGATGAGGGATCAATAGTTCGGGGCTGGCGGGAGGATCGGCGGCAACTGCTTTCCCAAAACAGTAGTGGCTGGAGAAAACTCGTTGATCGTTTTGGACGTAATGGGAAAGCTATTATGCAGCTTTAATTCAATCGGCCTGCTAGTTGTGTGCTCTGAGCTAATGCGATTGGCTCGTTGTCGGATTGTTTGTATCTCGGTGTCAAAGACTTTAGATATCCAGAGCCGGGGAGCTACAGGAAACCCAAACCGAAGCTCCACTCCTTCCGCCTGGCTATATAAAAAGCATTGTTGCCATCTCGGCCGGCAGTAGGAAATTCGAATTGACCGCGTTACCGTCACCGTCACCGTCTCCTTGCCCGCCGCAGTCGGCCGTCATTGATCCTCGTCACAATTATCATCGCATCGCATTGCTCATTTCATCGTTAAACGATCGGATCATTTGATTGATCGATCGCATGTGCGAAGGATTAAAGCGATGAGAAGATCGAAAGTGAGTGATCGATGTGCATGGGATTATTACCCTTAAAAGTGTGCCATGAATAGGAGTAGTGATTTGGTGGCAGTGATTTTGAGCATATCCTTGGACTACATGCTAATCGATCGATTTTACATAACAACATGTTTGATTCACAAAAGCAACTTACTGTTCGACTTC

TCGGGTCTTCACTTGAACCAAACAATCTTCACCTCTTACTTATCCATGACCGATAGACTTAACTTGCAGTGCCATCCAGTTGAAATAAGAGATTCAATCTTTATTACTGCTGATTTATTCCAAACTCTGTTCATAACACATTTAAAAGAATAACATGCAGACCACCACTGCAATACACAATTTAAAACTTGATTATTTCCTTGGATTGACTTGCATAACATTTACGAATCGCTTGATGAACTTACCAACATCATTCGATTAAAGGTCTAAATAGGCGGGACTTAAATTAGTATTAGATTAAAGCCCCAATCACAACAATCCCTTATTAAATTGAATAAGATGATCACAAAAACTAGGGCCTTACTAACCCAAATTGGATTCGAAAACGAAAGAGTGCAAATGAAGAGAGTTATAACTAGAAAACCAAATTTCCCAGATTGCCCGCTTTCACACAATGGCCGCGTTGGGCCTACTGCTGCTGGTGGGCGTGCATGGAGCCACGATAATCAGCATCAAGTACCCGGATAAGCCCGCCGTGGAGCCGCAGCCGGCCAAGAGGGATCTCAGCCTGAGCTACGCAGCCACCAACATCGACTCCAAGGAGGGTACCCGCGTGAAGACCATCACGGTGATCAAGAATGTGGGAGGCATTTCGGCGCCCGAAGAAGCTGCGGCCTTGGGATCCTCCATCGCCAGCAGCCACAACCACAAGCAGCAGCCGAAACTGATCATTGATCCCAGTCTGCACAAGAGCGAGGAGTGGGCAAGTGCTTTCCGAGACAACTTTGACTCCTACGGAGCACTGCCCGATGTGCCCGACATAGATGACCTATTCGAGTTTGTCAATCGCAAGAAGCCCGAGGGTGAAAAGA

 

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