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CRIMIC CR01782

Gene Ir68a

CG6185

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:11236218..11232972	68B3	FBti0212977	91305

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGATGGTGGTGCTAGTGTTGCCTAT	TTCTGGCTGGCTACCTCATCACATGA

Images

Construct Design Sequences

sgRNA sequence	GGCGACTTAATGGCTTTGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCCAAGGCCCCTTACATTT	GCATTTGAAACGCGTTCGGA	AAAGCCATTAAGTCGCCGGA	CGTCGACGGATAACTCCTGG

 

Amplicon 5'

Amplicon 3'

TGCCAAGGCCCCTTACATTTCTTCTCAGTACGTCAATGAAGACTTTTCAGTTTCTCAATTGCCATTCCGGTACTCAAGTACCCAGATACATTTGTCATTTGCTGCCCACCAATTATAAATATTGGGCATGTGTGTGCATATATCATTACTTTATGGAATGTTGTGCTCGATTGACAACTTGCAGTACTTGAGATTATGTACTTAGGGCGATTGAATTGAATGGAATGTTTTCTAAGTGATGTGCAAAAAAAAATATATATATTTCTAGGCAGAAACCTTGACATTAATGCTGCGGATATATTCGCTTAAAATTATAATACAAGCTTGGGTTGTACACATCATACATGGGATGTTTCATAACTATGTGCTTGAAATGCCATCTTTCAGCGAGCAGTGTAAGCATTGAGATTACGTAACCAATCAGCAAGGCCACAAAAGAGCTCCAGAATTTTTGAATATTCATTAGGGCCTCCTGTTGAGCAAGTGCCTCCGCCGGTAGATGCTCTATGGCATCCTTGAGCCACTTGGCTATCAGACCTGTGGAAGATGTCAAATGATAAATGAGATTCAGTCAGGATTTAAATACTGAATGCTGACCACCCTCCGCCAGATGTTGAATGGAGGCATCTACCCGTGGCTTCAGAGCCGAGTTCTTCTCCATGGCCAGCACTACGGGCATATAGAGGACACATTCCTTCATGATGTGGAGAGCTGATCCGGATTCGTCTGCCACTCGTAAGTAGCGCAGATAGAACTCGTTGTCGTAGTAGGCGCACTGTCCCTTGGCTATGCGAGAGGTCTACGAAAATAAATAATATTTAAAAATTTAAATTATATATTATATATACTACTAAAACCAAATAGCCATGACCCTCTTTCCGAACGCGTTTCAAATGC

AAAGCCATTAAGTCGCCGGAATGCTTTTCATATCACACTAGATTATTTTGCCAACTTGATGAATTAATACCGTTAAATTAAAAATGTTGCCAAATACGCTTATCACATAGATAAGATATTTTTCGTTGGAAACAAGTTGAAACTGGTTTTTGTTTTTCTATCTGGTGGTATAAAGTTCATTGCTACAAAATGTTGATAACAAGAGCGGTTATAAATCATGCAAGTTTATACGATTTTTCCCTAATTCTAAACGGTTCAAATCGAAATTGAATGGTCTAATTAGTCTTATGAATAATTAGAGTATTTAAGTCTTACCAAATCATCGCTGTAGCCGAACACCTCCATCTTTTCGCCAACTTTTCGAGCAACCTCATCATTGGCCTCCAGGAAAAACTGTCTATTCTCAGTTGCCCCGGTGGATGGCGGTATATGAGATCGCAGCAGATCCTCCAGTGTGTCTATAGTGACCCTATGATCAATAATAAATAACTTTCTCAAGATACAAATATGATAAAAGGATGATAAGCTTACCTGGCAGCTGGATTGGCCAAAATGGCAGTGAAGCTGGCCCTATATGTGGCCACCAAGAGGATGCAGTAGATCCAGTACCAACCAGTGAGTACCCTCAGGGGCCAATTTCGAGGCATTCGGGGTAGGGCCACATATAGGAGCATACTATACGTGAGCAGGATGCAGTTGGTATAGCCATCGAAAAGATCGCGAGGCATTCGATCTCCTTTAGATGAACGATACCGACTGATGGCAATGCGAAAACTAATGCGACGATAGATCTTTGGATCCATGGGCACGTTGCGATTTGGTCGTAGGCAACGAAAAAACTCAGAGGACACATTGCCGTTGATAATGGCATTCAAAAAACTGATGGCATAGAAAACAGTGCCCACCACGAAAAGGGAGAGCAGCACCCCCACCCACATTCCAGCGCTGAAGGGCAGGATAAAGGTCTGCCAGGAGTTATCCGTCGACG

 

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