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CRIMIC CR01790

Gene CG32112

CG32112

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:12745154..12741718	69E1	FBti0212978	91306

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACGAGGTTCTTCGAAAAGGGATGGG	AAAGTTCTTCAGGTGGGTGCACTCGT

Images

Construct Design Sequences

sgRNA sequence	TGTCTGTTTACTTTCAATAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATGTCCCTATTCACGGCT	ACATTAATCTCGGAATTTGGACG	CGCCAGGCCTATCTATCAGC	ATGGACTGGCTCATGACACC

 

Amplicon 5'

Amplicon 3'

GCATGTCCCTATTCACGGCTTTACTAACTTATTAAAAAATTACTTACAAATTTAGTTTTACTTACTTGAAAGGGTCATCTTATCTTAATATATGCTCTATATTATCTAAATTCCTCATAGTCACAAATTACCATCCGATAATTAACAAAGTTCATCTTGGTCTTACTAATTTGGAAACCTATTGAACTTCAGGTCAACATTTTCTGTTATATCATCGAGAAATGAATTTATGTTATTAAAAACGATGTGGTTCTTAAGTTAATTGAATTCTTTGTTAGAAACATGAGTTTTTTATGCAACCTAAGTACGTAGATTATTTATTTTTATCGTTTCAAGATGACATTAATCTGCATTACAGTGCCCTTCCACCAGTGGACACTGGACTTGACCACGATTTTAGATTCCTCCGTACAGGTTAGTGGGAAATATACCCTAAGCAAGTTAGGATCGATATGAACAGATAATTTTTTTAATTGGCAACTTGCTTTCAACAACTAAAGCTTAAAAAGTATTAATTATCCCTGAAGTTCAATCATTCACATAAAACTTATTTTTTTGCTCCCCAAAAGTGCTCATCATATTTTTTCGATTTTTCTACATATTTTTTGTACAATTGGAGTGTTTGTGCCCAAATTAATTGCCTTTGCCTCTGACGACCATTATTTCAGAGTATTTCAGTTCCGAAAAGTAATATCCAGTAGGGAGTCATGTTTCAAGCTCGTGCCCCGCGATTATCCGGTGGAGATAACGAAGAAAGAAATCGGTGCGGAAAGCACGCTGATAGAGGGCCAATTTAAGACGCCCATGGAGCTGCACATGCCCGGAGTTGTGCCCGAGGAGTCGCAGCAGGCGCACTTTCAGCTCTTGATCCCCAATAAGTGGAAAAACGAAAAGCACAAGCCCATATGCATTCATCTGGCCGGAACGGGTGATCATGTGAGTACCATCGAGCGAGATCCGTCGTCCAAATTCCGAGATTAATGT

CGCCAGGCCTATCTATCAGCATTTAGCTAAAAGTGGGTCCCACCAAAATAGATATTGTTTTACTTTTACCAAGCCAGTTTTAGAGCCCAACTTTACCTTTATATTTAATTTGTTTTCCATGATTTTCAAGCCCATTTGTTCGTAATTTGCTCAACTCTTTCGATACCCACATAGAAAACAATTTTGTTTGGGACCATTTTCAGCCATAAGTTCATAATAGTACATTAAAACGCACATATTTAAATGGCAAATTTTCAAATATTGCAGTTCTTTTGGCGTAGACGAAATTTCATTGCGAAACCTCTGCTAAAGGACGCCAATATAGGATCCATAATACTGGAGAATCCTTTCTATGGCTTAAGAAAACCAAACAACCAAACGTAAGTCTGAATAGTTTAGATGTATTTTATGTATAATAATAATAATACATTAATTATTTTAGGCGTTCCAACCTGCACAATGTGTCTGATATCTTTGTGATGGGCGGCTGCCTCATCCTGGAATGCCTGGTTCTCTTTCACTGGTGCGAAAGGAATGGCTTTGGACCACTAGGCGTCACAGGATTGTCAATGGGCGGTCATGTGAGTTTAAATTGATTAATAACCATTAAGGGGATTGTCAACAATAATCCTTTTCGTTTAGATGGCATCACTGGCCGCTACAAATTGGCCAAAACCACTGGTACTTGTGCCATGTCTATCCTGGTCCACCGCCTCCGCTGTTTTTACAACTGTAGGTGCTGCTATAGCATGGAATATCAAATTGAAATTGCATTATTCCATGTGTTACAGGGTGTCATGAGCCAGTCCAT

 

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