• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01791

Gene mus301

CG7972

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8142014..8137808	66B8	FBti0212979	91307

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCTTGGAGGCTTTCCTTACCAAAG	TTCAGGTTCTCCGTTTCCTCTTCCAG

Images

Construct Design Sequences

sgRNA sequence	TGTGGCCAAATTTGGTAGTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACATCAAATGTGGCCCCGAT	GTTAGGACGCGGTCACTT	GGCCACATCCTACATTATTGCA	TACAACTGCCTGGCACGATT

 

Amplicon 5'

Amplicon 3'

ACATCAAATGTGGCCCCGATTTGTTGGAAATAAATTCGGCTGGTCAAACGCTGGAGGAGATATTCGTCCCCAGTCGCAGTGTAGAGTACAATGTAAGGAATATTCGAATTAGACGTTTGGAAAATGTTGCCGAAAGTAAAAGATTTTGATCTAATTTTTATTTGCAGTACAGTGAAGCCGTCAAACGCGCCGATCCCGACCATCTGGCTGGTTTGATAAGCGAATGTGCTCCGGAACACTGCTGCTTGGTTTTCTGTCCTAGCCGCAAAAACTGTGAGAACGTGGCGCTACTTCTCAGTCGCATTGTGCCAAAGCATAAATTTTTTGAGCATCGGCGTAGCGAGAAGTTGGATCTAATGGATGCCCTGGACAAGATGTGCGGCATACTCAGCCCGGTGTTGGCGAAAACCCTACCCTACGGGATTGCCTATCATCATTCGGGCTTGACAACCGATGAGAGGAAGTACATTGAGACTGCGTATAGATTTGGAGTCGTCACAGTCATTTGTTGCACCTCCACTTTGGCTGCAGGGGTCAACCTACCAGCCAAGCGGGTAATCATACGTGCTCCTTATGTGGGTCAAGAATTTCTGACTTTGTGCAAGTATAAGCAAATGGTGGGACGAGCAGGTCGCGCCGGATTGGGTGAAGCTGGCGAGAGTATCCTGATAGCCCAGAGCAAAGACAATCTGCTGGTCGGACAAATGCTTTTCTCGCCTATGGATAAGGCTTTAAGTTCCTTGGATCAAAATGAGGCAGTTGGACTGCAAGTAAGTTTTTAATTAGCGAAAATTAGGTGAATTTTTTACGCAGATCTTGATGAGAAAGAAAATATTAATTTTGTTAAAACACTCATTTTGCATTTTATCGTTTGTTTAAACGTAAAAATTTCCGGAGTTTCTCTTATTTTATTTACTATTTTTTTACTTACACCGATGAATATTTCCTTAAATTGCAATTATTTTTTTAAAATCGCAGATTTTTATGTAGTTTCTTGCTGTCACTGACTTCCTAGGCGAAATCTGTTTAAAGACTGAAGAAGGGAAATGAAAAGTGTTACCTTTACATTTTTCTAAACAAAGTGACCGCGTCCTAAC

GGCCACATCCTACATTATTGCAAAATGTGCTTTTAATTTTGCTGATCCCTTCTATATAGATTTGTAATTTTTTTTTCAAATTGGCAGTAATGGGACAGAAGGCAGTTCTATCTCTTTTTATGCTTTTATTTGATTAATCCAAATTACGATATGCATAAACATAATATAATTTTTTTCCCTAGTCGCTTATTCTCAGCGTGGTTGGCTTGAATTTAGCAGAATGTCGTAGAGACTTGAATCGGCTGGTAAACAGCACACTGTTGTCTGTGCAGGCCAAATCCCTGGAGGTGGCCGTGAACGAGATCGTGCTCCGTATTCTCCGAGAGATGTTCAAAAATAAAGTGCTGCAGCTTGCCGAACCGCAGGCGAAATCTAAGATCAATTCTTCGGATATTATAACATCGCAGGATGTTAGTCAGGCAAATAGGCCCGCTGGCGATCGACGTTTGCTCATTGGACAGTCGACGCCATTTAAGCTGACCAATATTGGAAGAGCTGCCTTCAAGGCGGGCATCGACTACAAGCGTGCAAATGCCATTCACAAGGAGCTCAAGCAGGCCCAGCAGCAGCTCATTCTGACCAACTACTTGCACCTACTGTACCTTGTCGTGTGCTTTAACTCGAACGAAAGAGGAGACGAGTTGTTTCCGGCAGATGCCAGCATTCTGTTTGGAGTTTACACCTCGCTTCCACTGGACTCGCAGGCAATGTTCAAACAACTTGGATTTACAGAGGCCCATGCAGCTAGGCTATTTAAGACGCAGTCGGTGCAAGGTCCATTGTCCCTGCAGCTGAATCGCCTCTACAAGGTGCTTATTCTGGCGGATATACTTAACCTCCTACCGATTCCCTCCGTGGCCAGCAAGTACAACGTGGAGCGGGGAACTCTGCAGCATCTGATCAGTCAATCCACAGCAGCGGCGAGTGCCATTGTTCGCCTGTGCGAGGAGCTGGAAGAGTTTTGGTGCTATAAACCATTGTTTGAGCGGATCTTGCACAAAATGGATCGATGCGGCACATTTGAGCTGGAGCCGCTAATGGAACTGCCAGCGGTTAAGATCGTGAGTGGCAAAATAATCTTGTCCTTAACAGAAAAGTAGATATGACTATTCCAAATTGCATTACTCTTCTAGAATCGTGCCAGGCAGTTGTA

 

---

Search Again