CR01793

CRIMIC CR01793

Gene Psa

CG1009

Information

Donor Location Cytology FBti Stock number
pM37-p03L:1523380..150284362A3FBti0212980 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTTCCAGTCGTTCCACTTTCCAAGTG GTCTGGAAGGGAATCCTCCTTGATGA

Images

Construct Design Sequences

sgRNA sequence TTGTCGGCAGAAGGAATGACTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GACAGCTCCAGTTAACCCCC CCTTCTGCCGACAAAGAAATC GCGAATTGGACATGGCACTC TCGTTCAACTCGCCCGTAAA
 
Amplicon 5' Amplicon 3'
GACAGCTCCAGTTAACCCCCCGAAGCAGCAGAATCCGAAACCCAGCGGAGCGGCAGATAAGACCACCTCAGTAAAGCATCTTTTTCGAGCAGAAAGCGGCCACCTCAACGCGCTAATCTCGTCGAAGCAATTTGTGAAGGTGCAAAAGCAGTTCTACAGGGACTTCTCTTCCGTTTCGCTTGGAGCCCCTCTGAAGAGCAGTGACGCTCCGTTGCTGGGAGATTTGTGCAGTCAGTATCGCGCCATCCTCGCCCACACATCAGCGGACGTGATTCCAGGCCAGGATCGCCATCATCCGGGTTGCAACTATCAGCAGATCAGGCGGGCACACACACAGTTACTGAGCAAGGAGCAGCAGCTGGAGCAGGAGCGCAAGATGGCCCAGAAGGCAAAGTTCGAGAGGCTGCCCACCAATGTGGTGCCAAGGCACTACGAGCTCCTCCTGCAGCCCAATCTCAAGGAGTTCACCTTCACGGGCAAGACCGTGGTGCAAGTCAATGTGAGTACAGATCGCTATTGGGATTAATCGAGTAAGCGGTTTCAGTTAGGGAGTTGCGACTGTATCCTCCGTCTCTTTCCATTTCTCTCCGCTCCAGCCACTTTCACTTTTCCCGCATTGTTATTTTCTGCGCCTCATTGTGCGAACTTTTTCCCCTGACGTGGGCAAAAAAAATATTTCGGATTGGATCGGACATTCTCCTTGTTAACATTTCGCTTTACGCTGCAATTTGCATGCAAGCAAACGCTCAGACTGATGGGCAGATAGATATAGAGATATAGATATGGATATGAGTACACCCACTAGGAAGTGCGATTTCTTTGTCGGCAGAAGG GCGAATTGGACATGGCACTCGTGGGTACCGCGCAAAATTTATTCGAGACAATCTCTTTTTGCGAACGCCGCTCATAACGGTGGATTTTGTTTACGTTTTGCTTGCGTCAGCAGCGGAAATGATTATCCGCTCATAAGTCGCACTTTTAATACCTTAAAACTAAGATTAACTTAACCGAGATCAGCCTAGAATGCTACTTCTCACTAAATACCAAAATGGGAGGGTCTTTTGAATGCTATCAACTAAAAGCATAAACAAACTAACAGAACATAAAAAATTACAAAATATATTTAAGAGGGTATCTAAAACATGCTATTAACTGAGCTACTATCAAATAATAAGCATAGTTCAAATACTTAAAATTTTATTTATGTTTTTAAGATACATATCAATGAAGTGCAAAATTATTCATCTCTTATAAAAGACTACTCGTTATTCATATAAATCAACAAAAATGTGCTGCCAGCTAATGCAAATTGGTAATATTTTTCGAGCTTCATTATTCCGATAAGACGAGTGAAAGAATTACGATACCATTAGTTAGTTCATAAACTAGAGATCCAATCGGTTGGATCCTCCCTTGGTCAGTGTATAATTTTCGCCAAAAACTCGTTTTGACCGCTTTGATCGCTTGGCTTAAGCCGGAAACCTACAACAAAGTCCAGAAAGCCGCATTTCTACGACCTTGAAGTAACTGTTTGTGGATTCTGGCCATTCTTGTCGGCCAACTCGGCTAACTGTTTCTGCTGACTGGTTTTGGGCTTAAAGTTTCGGGTTGCTCACCTAACGGTTACCGATTGTCTTGCAGGTCAAGGAGCCCACTACCCAGATAACCCTGAATGCCCTGGACATTGTTCTCGATTCGGTAGAGCTCCATTTTGAGTGCAGCAAACTGAAGCCGGAGAAGATAGTGTACTCTGCGGAAAATGAGACGGCCACGCTGGAGTTTGCCCAAGAGATTCCGGCGGAGACTCAAGGCGTACTGCATATGTCCTTTACGGGCGAGTTGAACGA
 
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