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CRIMIC CR01809

Gene CG42245

CG42245

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:2641984..2631715	62E8	FBti0212982	91309

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTTTCCCACACTGCTCGATAAGCAT	GTTGTCCGAGGACTTTCTCTAGCAGA

Images

Construct Design Sequences

sgRNA sequence	GCGGGAGCGTCGGGCGAGAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGGCCGGGAGATCTATCCG	TCCCGCTTCACTCCCCCT	TTCCCAGGGCCGAGAAAAAC	GACAGCGTCATTGCGAGTTG

 

Amplicon 5'

Amplicon 3'

TAGGCCGGGAGATCTATCCGCCGAAGAGGCCAAAACGGAGAAACTGTTGGCCTCCAAAGGTCGGTACATATGTACAAGCATACATACATACATATCGCTGTAGTACATACATACTTACACGCTCGCACACGCACACACACATGCATATAGCCCACGGTGGCGGCGGCGGCAGTTGCACGTGTGTGCGTAGGTAGGATGTTGTAGTTTGTCTTTCAGTTTAACTTTTCAGTCAGAATCGAGCGGTTGTCTTCTTCTGCCCGGCGTTACATATATCTAAATCATATATGTATATATACATAACTGCGTATATACCGTTGCTTATGTATGAGTGCGCGCGTGTGCTTGTGCATTTTTGCCAAATGAAAATCTAACGGTAGTGCGAAAACAGCAGTTTGTTGCAAGAAGTATGTGTTTTTTTTTTCGAAAAGCACCTTCGGTTTTTCAATCTTTTTAAAGCCCTATGTTGCCATGGCAATCTTGGTCATTTCCGTCCTAAAGATTCCCTTTTTTGCCCAGCGGAGCACCCCTCACACAGACACACACACCCGCAGCAGCTAGCCGACGAGGCATACACACTGACATTTTCATTGGGGGCTCCGCCAAAGCAGAGCATGTGATGAAAATCGTTGGCAAAGCGGGCGCGGCGATAAAGCAGCCGGCAAAGAAGTTAACAACAGCAGCTGCCGCCGCTCGCTTTGTCCGCCTCTTCCGCTCGCCCCCCTGCCCCTTTCAGCCCCACTTGGAAGAGCGCAGAAAATCGGGTGAAAACAACGTGTGCAAGAACGAACGGATGAAGGAGGCGGGCAAAGCGGAGAGACAATGATGAACGAAGCAGGCGAGCAAAGAGGAAAAGAGCGGGCCAAAGTGGCTTGACGACGTGGTGGCGGTGGCAAAGGCGCTGCGGGGAGGGGGTAGGCGTTGAAGAAGGGGCTGGCGGAACAGCGGGGGCGGGTTGCGAATCAGTGGGTGGAGCGGGAGGGGGAGTGAAGCGGGA

TTCCCAGGGCCGAGAAAAACATCTTGTGGGTGCTGCTTGGAAAAGGGGGGGGTCGGTGGGTGGCTTGTTCTGATTTACACCTCCCTCCTCCCCATTTGTCATGCTGCAGTTTTTAAAACCCAGATACATTTTCCAATCCAATAATGAATTGCTGGCATATTAGTCACCTTCCTGGCAATTTTATAAGCAGATCAATTTTCAAAAACTTTCTTGTATTCCTTTTAGTTAATGCAATTTTTAAATTTATTTTATTTATATCAGCTTTGATATATGTATTGGCATTTTTCTCCCTAAATTTGTGCATACTACTTTCCCCAAAATATTTTTCATTCTAAGATAGTTTATATAACTTAAATACTCCATTTAACTTGTAAATAGGTGTTTACTTACACGCTTTACTACGATCATAATAAGTACAGATGATTGCTGACCCATAACTAACTCCCATTTTCAATCTTCCAGAATTAGCCGAAAAGCGAGCGGATCTGACGCAGCCAATAGTCTTCAAACACTGTGTCAAGCAGCCCAACATCGAGAAAAAAGAGTCCCAATCTGAAACCAACAAGAAGTCCGAAAAATCCAGCACATCCAAGAGCAAGAAGTCCAAGGCCGCCAGTAGTAAGCTGTCCTTCAACGAGGACGAGGAGGACGAGACTGCCGAGGATTGAAGATCACACCTTAGTTATCTGGCTATAAGTTGAACCAACCAAGTTAACCTGCAGCGAATGATTAAGGTGAGAAATTCAGGTCAAAAACAGCAAATGATTGTTAACTATGCTTTGTATTTCTTTGTTGCCATGTGAATTTATGAGTTTAATAATGGGGTTTCCATATGTATGTGTTAAAATCCAACTCGCAATGACGCTGTC

 

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