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CRIMIC CR01811

Gene CG11353

CG11353

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:4533170..4539270	64B9	FBti0216170	92657

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCGTAGTAGTGTTGTGCGTGT	TCAGTGGTTTCTATCGAACCCACCTG

Images

Construct Design Sequences

sgRNA sequence	GGAGATTGTACATACATTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCCTGAACACCTTGCAAAA	GCCTCTAGGGTCGTGCAAA	CAATCTCCAATCAGTACGCAGA	TAGCGCATACAGATCGCAGG

 

Amplicon 5'

Amplicon 3'

CGCCTGAACACCTTGCAAAATACATTTTTTTACGAACTTACTAATAGTCAAACAAAAGATGCAACTCATTCATAACGAAATAAAGACGTTAAATTTTAATTATTGCTAAGAAAAAAAAAACAAAAACAAAACAAAAAAACTCGCTCTCGTTTTCTTGTATTCCTCGGTCATGAATGGTTATTTAAGCGGCTACACTGCGTATGCGTAATATTCTAATGCAATCGGCAAAACCAAAGATAAAAGCGTTGCTACTTTTTAGTGTGTGCAATGTGTGTCAGTGGGAAATTACTGAGCCGCGTTTGAAAACGTGCTTATTGGCCATCGATGGCCGACGAAAGTTTCTCCCCATCAATTGGCAAGCATTAACCAATATAAATATACCACAGGCATCTGGATCCGGGAGGGGCGAGAAGCACAGGCGTGGCCAGGTGAATCGCAGTGAATCGCAGTGCGGTGACCTTCGGCAATCGACGGCAGCAAACAGCAATCGAACGAGCCATTTGAGATAGCCAAACAAGCGCGGTCCAAATAACTCTCACAATACGCGCACTAACAGCAGCTAAATAGATAAATATAAGCCAAGGTTACCAAGTACCCACCGCCTCAGCATGGAGATCAGGCGCACCTCTCGACCATATCTTTGGTACACACCAGTGCTGATGCTGCTCGGCCTTTGGCTGGGTTCGGTTTTGGCCAAGGGCGCTAGCAATCAGTCGGCCACTACCAACTCACACGCCTGGCAGCGATATCACAGTCAGGCACTCAATGCGTCGCCAGCGCTGCCCGATGAATTGGAAATATTGGATGCCCCCTCTACGATTGAGATACATTCGCCTCATGAAACCCATTTGACACGCACCTCCGTCATTTTCGGACTGACCAAAGTGGCCAACGAGAGCAGCGTCAGCCAAAAGTGCCACGCGCAGCTGCGACAAATGCAGCGCGGCATACTCGGCAAACAGCCATGGGCCATGAAGGGTGAGTCGATAAAAATAAATACATATGGTCACAAGGGGAGCGTAAAGTCCCCAAGTACTTGTTGACTTCCTAAAAAAAAAAAGTAAATTTGCACGACCCTAGAGGC

CAATCTCCAATCAGTACGCAGATTAGTTTACTTGAAATGCAAAATATGCGATTCATCAAATAAACAGTTTTCTATTAAAATTCGCACCTAAGTAAGTTGCATGACTACAAGTGAACCGTACGAGAAAACACTTCTAAAAAATTTGTTTAGATAAGCGAATGACTCAAGATAAAAAATAATGCAAAGATTGTAAATTACCAAACTTCTAAGTAAATTTCAAAACTAATAATCATGGACTTTATATTTTGAAAATACCATTACTCATGCGACACTTGAATTCGACAGCTTAGTTACTTGGGCACAAAGAACACTTAATTTCAGATTCGCTCTGTAGGTTGAACCCTTGATCACAAAAGCTATTAACATGTTCCTAATTGAAGTGCTATTGAATTTTCCCACAGTTCTGGATGCTTCGGGAACAAAGCCCTCCGGCTTTGTATATGGTCAAAACTACTGGCTGGGAAGTCGCGAAGCTTGTCGCGGCGTCCAAAGACCCGTGGGCATAACCCTATCAAAGAACTTCGATCGTGTTATGCACTATGGAATTATCACCCAGCAGGCGCCCTTCGACATGGACTATCGGGTTTTATACCTACGTCACAATTCTCCGTGGCAAGTGGAGATTAAGCTGATGTCTGAGCAGATTATACACATTGGCCTGTGCCTGCCAAGTGCCTGCAGTTCGGTTGAAGTTCAGAGTCTGGCCCAGGACTATGTGGCTGGCGGAATGTTCACTGAAAACGAGATATTCGACATAAAGCCGGAAGTGGTTTACATGAAGGATCTGCAGCTGAAGGATGAATTCTTCGAGAGGGCCAGCTTTCGTCTCTTGGTGGCCTGTGTTCTGCTCACTGTCACTCTGATGTTTTGTGCTCAACAACTGCATGCGACTAGTAAAATGCCATCTATTTCTGTGGATCCTCTGGATCGTGACTTGGCACCCGCGGAATCGGAAATATGGCGTGGACTTAACTCCCTGCTCCAAATAGAGAGACTGCAGCGATATGTAACTTGCTGGGATGTTCCCGGCAATTGGGCCAAAATTTTTGCGATTAGGGAGAATACTGCGAATGAAATTCCCTTGATGAATGGCCTGCGATCTGTATGCGCTA

 

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