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CRIMIC CR01818

Gene MYPT-75D

CG6896

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:18667177..18625959	75D4-75D5	FBti0212984	91311

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCAGGGAGCGAATCGAACAGCTGAA	GCCTGTTTCGGTTGTAACACTCACAC

Images

Construct Design Sequences

sgRNA sequence	TCTACCCGATCACTCCCAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTGCTACGCCTGAAAGCT	TCGGGTAGATGGGATGGAGG	TCTGCTAACTTAGTTCTCGGGA	CGAACCCCGTTCTCAAAACC

 

Amplicon 5'

Amplicon 3'

AACTGCTACGCCTGAAAGCTGCAGGTGCTGCGGGAGAGATGCAGCGAGCCACCTCGGCGGCGGCCGTCATTCTCACCGAGAAGGGTACGGCGGCCAGTGCCGAGGCCGTTCAAATCCAGTCGTCGAAGGAGGCTGCCAACGGCAAGATCAATGTCCAGGTCACCGTCCTCGTGGGTGAGTCAAGCGCCCTTGGCCACGTGTCAGGCTTGCTTCCGCTTTCTCCTTTCAACCAAAGCCAACCATTTACTCAAGCACCCCATCCAGACGCCACCTCCAACCATCCTGTTTGGCTCCCAAGGAGCGTCAACGTATCACAAGAGTTCTGCTTAAGCGCCATTCTGTCCCCGTGTCAATGTCAATGTTTAATGTTTATTTAACGACTCTACTAAAATGGAAATGAAAATACTTCCAACAAGCAAGCAGAGCTAACAGCATGAGTAGACTATAGAATGACGAATGCCCTGTAGGAAATGATAATCGATATCTTGGTTTGAGATTTTTAATAGGATAGCTTCGTGAGATGCATAGCTTAGTTGAGATATATTTTATGGAAATGACAGTCATTGTTGTAGTAAGATACTTTTTATTTCATTATGTTGTAAATATGCTTTGTAAATGACTTCTTTATTTAAATGCCTAAAGTGGAATAGCGTAAGCATATATTACTATTTATGTAAATTATTGCCATTTTAATAGCTTACTACACTAAATGGGTTTTCTGATACTAGAAATCGTACTTAGAAAACAGGGGAGCATGGCTGCAATAAGCATTAGCATTTGACTCGCATGTAACCTCCATCCCATCTACCCGA

TCTGCTAACTTAGTTCTCGGGAAAAGAAGAGAAGAAAAGTAAAATAACATTTGCTTGTTTCCGTTTCATTCTTTTTTGTTGTCGTTGTTGTGCCTCGTTTCGCCCGTTCTGTGTCGTCTGCCTGCCCGCATCATCATCAACAATAAAAACAACAACCACCGCACCACTAAACACCAAACACACCCAACGCCTTGCCAAAAACTTCCAACATCCAGACACCAATAGCACGCACCACCATCAGCAACATCAACAGCAGCAGCAGCAGCAGCAGCAACACCACCACCAGCAACACGTACTGCTGTTGCAGCAACACCAGCAGCTTCAGCAACATCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCATGTTGCCTTGGATGGCTACAGCGCCACATTGGCCAATTTGAAGAAGCAACGCTCGCTTTCACGCACCGCCAATGTCCTCAATACTTTCGAATTGTCATCCCAAACAGCTAGCAATGCCCATCCAATTGCAGCAGCTAATGCCACAACAACTAATGGTTCTGTTTTAGGAGCCGGCGGCAGCAGCAGCAACAACAACAACAACCTGAGCACCAGCAACAACAACAACAACGGATCCGCACCGATCTCGACGCAGCCAACATTGGGTCACATGGGCGCGGGAAGCGTGCTATCCGACTTCGAGGGCTCCTCGCCGGCGGGCAGTTCGCTGAACAAGTTCAGCGGCATCACTGGCGACGTGGTCACCGATAGCACCAGTTCCAGTCGCAGGTGTTGCGTCCTGATGTAGAAACTTAGCGAACTACTAGAGTCACAGGGTATTAGAGCAGCCAGAATTGTCAGAGGGATAGGCCATTTGTTAAATGGCAAGAAAAAAGTGTCTTTTTATTAGATTAGCCGTATTCAGTGAGTGAATTGTTGAATGTTGATTACACAAATGCATGTTGGTTTTGAGAACGGGGTTCG

 

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