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CRIMIC CR01822

Gene CG5644

CG5644

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:8979880..8977395	66E6	FBti0212986	91313

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCAAGTGCACGAATTGGTGGGC	CCGTGAGATTCTGTTCACTCATCACG

Images

Construct Design Sequences

sgRNA sequence	TTGCGTGCGTGAGAGCAAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTCACCATGGCAGATCACA	GCAAGGCCAGCACAATTCAT	TATTCGCCGCCTCTCTCTCT	AGCGGGTTCTCGTTTCGATT

 

Amplicon 5'

Amplicon 3'

GGTCACCATGGCAGATCACACTGTTTGGAGCCGCCGCAGTGGCATTAATCTCGTGCTTTAGATTTTCAAACAGATTGGTACGAAGTTCCTCGAGCAATCGAATGGGCGTGGTCAAGCAGTCGTCTGCATTGGCGTCGCCTAGACTTTCCAGAGCCTGCTGCACGGAGGTGTCTAGAATGGTGGCGTAAAAGTCCGTCGCCTCATCGCAGTAGACAATCTCTTGAAGATGATCAGCATGGAAGGCGAAGGAAGAGGATTCCAGCTCTTGAGCGGCCAGCGAGGCAGCATGGAGTTGTGCAAGTTTCTACAAATAGATGACACTCAATAACTATGGCTCAATGACTACAAGTAAATTCTTTCACTCACTTACCTTCATCACTAGAAGGCAGTCGCTCAGTTCCAAGCCCGCCAGACGATCCTTCATGCGAAAACCCATGGCTTTGAGGTCCTGAAGCACGATGATGGGTTCTCCACCTTCCGCATCCCGCCGATCTTGACTCTCCGCAATGTGGCATACCGGAAACAACTGATGACGACTGTGGGCAGCTAATAGGGGCGCCAAGTGCCGATAGGCGTTGATCTCGTTGCTGAAGGCCTCCTCGCATCGGTAGAGTTGCCGGCGCGAAAGGCTCGCAATTTGTCGCTTCACAATGACCGTGACTAAACAGAAAAAAAGTTTAATTATCAATTTAATATGATCTAAATATTGCATTCTCTATCGAAATTCAGTGTTAAATAAACAATGATTCATATCAAATCATAAAAGAGATGTGTTATCTGAGAATGATTTGTGAAAGAGGGGGTGGGTGAGGAAACGCCGAGCAAAACAAACTGTGAGAGCGGCTGGGGCAAACAGCTGACTTTTGTTTACGTCGCCAGCTAAAATTGTAATTTATGAATGAATTGTGCTGGCCTTGC

TATTCGCCGCCTCTCTCTCTCTCTCTCTATCGCGCGTGTGTGAGTGTGCGTGCTGACCTTTAGCAAATCTATATTACGTATATGCCTATGTGTATGGGTGCAATGCATTTAGAATGCGTGTAACAAGGTCAACCCACTCGACGCCGCCGCTCAGCGGGCAAACATTTCAGCTAATATCGTTGCAATTAAGGTTTGAACTGAATAATACTCTATTCATGGTACAAAATAAATATTATTGGTACAAGTACACATAATATTTCAACAACTCGATTAAACGAGATCTCAACGTTAACTAAATATTGCACAACAAAATTTCTCAAACAATGCGATCTAACAAAGAGTACTACCCTTAATGCAAGCCACATTGCAGGGTATTAAAGAGAAAATCAATGCAATGGACTTAAATGTTTTTGAACCACTTTACCGATTTCACTGCCAGCCAGCTCCGGATCCTGATCCTTTCCTGCCTGGGAGATGGTTACACGCTTCACCACGGACATATAGTTGTCGCCACTGCTACTGCCCGCGGAGCACGCGATGTGGGCAATGCTAAAGGACACCAGTTTGCTCTGGCTGAACAGCTGACGCAAGTGCTCAGCGTTCTCCTCGTATTGATTGGTACCATCGTAGCCACCGGCCGAACTCACGGTCAATGCCAAGCCGCCATGCGAAGGTCCTGCTGTTGGTGCCGCTGCCAAAGCCATTCTATCCACTTCACTTGGCTGTCCGACGAGGAATGGTTCGTGTTGGAGGGCTGCTGCCAAATCGTTTGCTAGAACTTGGTTTCTGCCGCTGCAGCATTTTCTTTCTAGCACTGACACAAATCCAAATTGAGTCCAGGTCAGTTTTTTTTTCCGTAATTATTATACAATTTCCGCTGGCCAAAGAATTGGTTATAGCACACCTATGCGAAACGTGTGCAAAATGGTGCAGCAAACCAACCGCCCGCCGAACTGCCAAACGTCGACTGGCAGTCCAAGTGCAATGGAAACTCACTAGAGAGCCCAAATAGCAGTGAGAATCGAAACGAGAACCCGCT

 

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