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CRIMIC CR01828

Gene CG43219

CG43219

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:21223118..21220400	78C2	FBti0212988	91316

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTCTCTCGCTTTCTCCAACCTCTC	GGCTTCCACAATGTCAATGCCAAAGG

Images

Construct Design Sequences

sgRNA sequence	AATGGGCAATGGGTGGCCGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCAAGAGCATTTCCGCAAA	GCCACCCATTGCCCATTTC	CGTGAAGAATGACGTTGGCG	CTAGTTCAGGCCCGGCATAG

 

Amplicon 5'

Amplicon 3'

GGCAAGAGCATTTCCGCAAAGGATTCCCCATAAGATTGGAAAAGAAACATCAAAGAAATTTGGTGAAGTGAAGTGCATACGTGGAATAAACGAAAATTTAAGGAACCCCAAAAGCTTTACATTTTAAACTTACCCACACAGCCAAACGGAAAAACACACACACTTTATCTAGTGAACACTTTATCCAGTGAAAGTGAAACATCTAGTGGAATCTTTTAGGCGCAGCGCAAAAAGGCCGTTCCGAATCCTAGTACTTTCACTTCGAAGGCGTGCAATAATATTTGTTTGGACCAAAATCGGTGGTGCAGCTGCTTAAATGTATTTTAGTGACAGCTCGGAAAAACAGCCAGCCCCTTGGAGGGCCGGCAATCCATGGAGTATTCAAACACCACGGGTTTGACTCACAGCATCAATGAGAACTCACAGAATCCGGAGCAGCAGCAACCCATTAAAACCAGGTTAGTACTTAAAAATAGTGCGCAAACATGCCCCATTTAATGACCACACCTGAGCCAAACTGGTTGCCCCCTTTTGTGCTATCATTTTTTTCCTGTTTTTATCTCCCTTATTTCAATTAACCATACAATTGCACGGGAAAAAATGTAGGCCCGAAGAGAATGGGAACTTTTCAGCCACAGTTTGCCGCAGTTAGTCCATCATTCATTTGGCTGACGAGTGAGCCACTAACCTACTTCCTTCACACACAAAAGCCGCAGTTGCAGGCAGGCAGCATCTGCTGTGCTGCAGCCGTGGTTATTGTTGCTGCTGCCGCTGCTGCTTCTGCTGCTGTAGCCGGCTTGCCACGCCCACACAATGGAAATGGGCAATGGGTGGC

CGTGAAGAATGACGTTGGCGTTGATGAGTAAAAATGTGAAAATTACATGGCAGGCGGATGGAAATAAACGACGACAGTACATGGCAAATGATGAGATTTACTTCGCAAAAGGCGCAGCCTTTGAACAAAATCTACATATAAATATAAATGAAAATCCTCAATGCGATCGATGGCTTGCCCGAATAAATCAAATGTTCCATTTGGCAGATATTAGAGCACACAATGCGCGTTGGGAAGAACCATCACGAAGAAAATGGCAGAGGACTACTTGCCAAGACTAAAACTTAAATGCCACATGGGCAAATATTTCACTTGTGCTTTCGAGCGGCGCATTGAAAAGCGAAAATATTGTGACAATATAAGCAGCAGGAACATCAGTAACCCGGGACCAACAACAAAGGCATGCCCGAGTGTCTTCCACTTGTGTGAAGTGCATAACAACGTGGAAATATTGCAAACTACACACATGCAGGGAAATAGCAACTAAATGCAATGACTGGCAACAGCATCACTAGCCACAAAGTAGAAAAGGCAAAAAAAAAAAAAAATGGAGAATACAAAAAGGACATGTGGAAATGCGAAAAGCAGCCGCAGGATGTGTGAGTAGATGGCAGGACGACGGCGCATGCAAAGCATCTATTGATTTTTATCCTCATGCTGCAAGCGCTCTCTTTTGGTTTGGTTTAGGCCACAGGGCGTATGAGTAATGCAGATTGCGTATACTTGATTTATACGGCCAGCTAGATTTGTTGCTTCTGTCATGGCCTAGCTGCTGATGGTGCCATCAACTTCAGATGCATGTGCAATAAATCTCATCAAGCTGAACAACTTTAAGAATTGTTAAGGCAAACAAAAACCTCCTTAAGTTTCAATACAACCAAACAAACAATCGATCATAGTGGGTGGAAAACTGAACGTTGCAAGGCTTTGCCTTCGCCCGCAAAGCGCCGATGCTCAATAAATTCTATGCCGGGCCTGAACTAG

 

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