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CRIMIC CR01830

Gene l(3)72Dr

CG32154

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:16239462..16237723	72D10	FBti0212989	91317

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GAAGTCTCCTTGTATGGCCACCCTTT	ACAACTACTCTGCGAGTGGAGCAAAG

Images

Construct Design Sequences

sgRNA sequence	AGTGCGATTAACGCATGAGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGACGGGCAAGGAAAGATCC	GTTAATCGCACTGCATTCTGC	AGCGGGGAGAGGTGTTCATA	CCAGTTGGAGCCCTTGAACT

 

Amplicon 5'

Amplicon 3'

GGACGGGCAAGGAAAGATCCATTGGTCAAGTGATTCCGATCAAAAATGTATATAGTTAATAGGGTCGGAAACGCTTTTTTTTATAGCTTTGAAGTGTATTAAGTATATCATCTATTTATTATACATATAATATATAATTCATTTTTTTTTTTGGTTTTTAAACGAAATTATTTAATTTACAATAATTGACTAGCGTACTATTACCCTTTTTAGGAAACGTAATCATAATATCATAATTCATATATTTATAATTATAGATTTATAATTCATATAAATCATTTACATTACTTAGCCACTATAGCTTGGCTCATGATAAATTTTATCTTACTTATAATTCCAACGACAATGGTTGCAAAAGTACAATATATTGGCTAGGTTATTGTTTTTCTCTCGCCGACTCGAATGCGAGACTCATTTCTATCTGCTCGTGCCCGAATAAATAGGAATGTATGGCATTTATATCTGGCCACTAGCTCGTAGTCTGAAATCCGAAGAAGACGATGGCGGAAAGCGCTTATACCGATGCGCCGTACCTCAACCGCGTACCGACAGTTGGTGTGATGTGCATCGATATCGCCACGCAACTGCAGCAGAACTTTAGTGGTGCATATCACAGCTATTTGGCTGCATCCTATGTCAAATTTTTGGAGGCATCCGGTGCGCATGTGGTTCCCATTTGGTAAGTTATAAATTTTCAATCATTTTGCAACACTTTGACTTTTATTTTACCAATTTTTACATTGCTAGGAAATAATATGACAAATATAAAAGGGGACAGAATCAATTCGTAATCTTTTTGTATGGTTTAATGTGTATAACCATATTTTCATTAGCCGCAAGCATTCCAAGCATTTAGATCGAAAGTCACGTACCAACAAATAGGGCAAAAGCGATTCGTATGATTGGGGTGTCTCAAATCTGCAAAAGCATCATGTGCATCGATTTCGCCAAGTCACTGGGCCACACTTTATCTGCCCGCTGCCAATTTGAAGCACTTGGATGCCATTGGGGCATTGGGTGTGCCCATCTGGTTACCAATAAACAAATTGTATAAACAAACGATGCACGAGAATTAATTACCTCATCTGGCAGTTTGCCAAAATAAGTTTTGCAGAATGCAGTGCGATTAAC

AGCGGGGAGAGGTGTTCATATTTAAGGCAAATTTATTGTAGTCGGCAAACGAAAGTGCTTTCGATTTCATTTGAAAACGCGGGGGCATTTAAATATCATAAACACATACGTTGAATAGTGAAATTTTTTAATTATTTATTTTTAGGATCGGCCGAGAACGAGCTTACTATGCGTTGATGATGAGCCAGTTAAATGGCATTCTTCTGCCAGGTGGAGCCGTGTTCATCGACGAGGCTGATAGGCAAGCCAATCCGGATGTGACCAGCGATTGCGTCCGGAGTGCCGAGCTCATCTACCAATTGGCTATGGAGCGTAATATGCGGGCGAAGAAGTTGGATGATCGTGGCGCATACTTTCCCGTTTGGGGAACTTGTCTTGGCTTCCAGTTGATCCTTATTCACGCTGCCGAAGCACCGAATGTTCGAATCGCCTGTCAGCCCATGAGGGAGGCGATGCCAGTTACTCTGACCGATGACTATCAACAATCACAACTGCTTGGAAGTCTGCCGAAATCGGTTGCCGATGAGATGGAGAAGCACCCATTTGCCTGCCATCAGCATCGGTATTGCATCACTAAAGAGAGTTTGGAATCCTATGGTCTAGCTAAGGATTGGCATCCCCTGGCCACTCAGAAGGACACTTCAGGACTGGAATTCATCACGATTGTCGAGCACAGGCGTTTTCCCATCTTTGGATGTCAGTTTCATCCTGAGCGCGCTGCATTTGAGCAGCTTTTCAACTCACCGGACAAATGTTACATGGCGCATTCTCGCATGGGTATCGATTTGTCCCAAATCTTTGGCAGTCGGTTTGTTGACTTCTGCCGCCGCAACAACAATCAGTTCGAAAGTGACAAATTAAAAACCAGACATCTCATTTGGAACTGGCAGCCCGTTTTCTCTGGCAAGTTCAAGGGCTCCAACTGG

 

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