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CRIMIC CR01831

Gene cyc

CG8727

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:19815938..19813770	76C6	FBti0212990	91318

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCTGCTCCCATGCCATCATGATCTA	TGTGGAAGTACTCGTAGAAGCTGGTG

Images

Construct Design Sequences

sgRNA sequence	AGTGTGGATTTCCCGTGTAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGTACTGTGGAGTTGCGAA	CACGGGAAATCCACACTGG	CTTCCTCTTCCCTTCACACCC	AGTTTCTCTTGGACCTGCGG

 

Amplicon 5'

Amplicon 3'

GGGTACTGTGGAGTTGCGAACGTTGGGCGGAATGCGACCAATTGCCACAAGGCAGGATAGATTCGTTGTTTGCTCGTCGCTGTCGGCGTCCTGGTCCTCGTCCTTGATGGGTGTCCAGGACTTGAGGTAGCCCGTGCACTGGATAACCCGGTACTTGTGGCCCGTAGTCAGTCTGGACTTGCGCTTCGTGGAGCTCCGGGAGCTGGAGGACGTATCGGACTCCTCCTTGATCTGGTTGTTGCTGGCAGTGCGCAGCTTCATGCGGCAGAAGAAGGAGCGCCGGGCACCCGGACACAGGCGGCACAAGCTCTGTGGAACGTCGGTCTTAACGGGCAACATGGCTGCAGGTGATAACGCAAGGAGAAAACTTAAGTGACGTACTGAATCTCACACTTTCGCATGGCCACTTACTCTTCGCATCGATAAGCCTTTCCCTGGGACACTGTTCCAGTGAGGATAGCTGCTCCTTAACCTTGCCTATGTCCTTCGGATGCAGGACGTCGAACCAGCTCTGTCCCAGCAGGTCCGCTTGGGTGCTGTTCAGCACACTGGACACCGAATCGGAAACGTACAGGATGCGTCCTCGGTCACAACCCACCACGAACAGGAATCCCTCCGACGCTTGCAGGATAATCATCTTGAGCTCCTGGTCGGACAGGAAGCTAGGCCGGTAATCGGATCCGTTGAATGGATGTAAGCTGCCGCTGCCACGGATCCCTCGCAGATGCTGCACTGCCATCCGGAGCACAGTCAGTTTGTCCAGCTTTCGCTGCATCGCAAAGCACATGGGAATCATGGAGGAGAGCTCGTTGATGTACGTGTTCATCTTGTCCCGACGCCGCTTCTCGATCTCGCTGTGATTTTGCCTAATTAAAATAAATGATATAACGTTAAAATTATAAATGCATATGGATGCAGGAATCTGATTGAAAAGACCAATTAACTTTTCCGAAATAATCATTATTAGTCTTGAAGTCGAATAAATGGATATGGAATTAGTTAAGCCTGAATAATGTTTTCCGTACTTGCGATTTTCATCCGAGGTTCTAGCTGACTTCTCCTCGTCGTAGTTTTCATCTTCGATCTCCTCCATATTTTCGCTAAATCGAAAACAACAAATGGTGAATTAGAAAACAACCCCGATATTTAATCAACCAGTGTGGATTTCCCGTG

CTTCCTCTTCCCTTCACACCCACACGCACACCTGGCCAACTTGTATGAGCGTGTGTGTGCTCGTTTTCGCGATTGTGCGTTCGTGTGGGTGCTTAACGCACACACTTACAGCTCGTTTTTATGCAATGTGTATGTAATTTGCAATGCACTTTTCCAGTGAAACTCACCAGAACTCCTGAACTTCCATCGCCAATCGGTGACGTTTGCTTATGAAAAATTAAGCCCTTTCCGACGAAGACCTCCCTTTTGTTTGTTTACTAACATCGACTAGCATCAACGTCCCAATAATAACATTTAAAACGGAAGTTTGTGCAGGCCGACAATTTGTTGCAAGCTTTTCCTGCATCTTCAGCTTCGAAGCTGCAGTGTGAACGTGGCTGCAAAATATACCAGCAACTTTCGGAAGAATACCAAGGGAAACAAGCTTTTCGCTTAAAAATACCATTTGGCGCCAATTAACAACGGTATTTAAATATTTAAATATGAAACTCATATGAATCAATTTTCATTATTTTACTTTGGCTAGACAATCTGAGTAAAAATCAACACAAGTCGAAACAAACACAAACAAACAATTAAATACTATTTATATTTGATCCTTAGTTTAATCACATCTTAAATGTGATACATTTTATCCAAGCATATCGTTATGACCTGCATATAGCGATCCCAAAGACATCTGGTTTCTTGGTCCCTGCAAAACATTTAGAGTTGATTATCAAAAAAGTTACTTGATTAATCTAGACAAAAGTACCCACCTTGATATTTCCAGATCCAAAACGCTTTGCGTTATGGAAGCCAAAAATTTATTCACTTGCTCACGGTTCCAGCCATTCTGACGCCAAGGATCAATAAGCTTCTCACAGAGATCGACGAAAAGATCGCGCAGCTCCCGATTGCGATGCAAATTGCAGGCGATGGTGATCAGGGCGCGCCAGTAAGAACTGCAAATCAAAACAGATCAATAGTAAGAAAAGGTGATAATGTCGAGAAATAAGTCCTTACCGAAAGTTGCCATCCAGTTCGTGAAAGGCGCGCTCGAGAAGAACGGGTTTGAGGCGCATGCAGACGAGGCTAAATGCAAGGACTAAACTATATAATAGCTCGTGTGCGGTAGAAAAATAAATCTCACTGCTTAATCTCCTTTTCCTTGTCGAGTAGACAGCGCAACTCCCGCAGGTCCAAGAGAAACT

 

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