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CRIMIC CR01833

Gene CG12004

CG12004

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:1576330..1569181	62A6	FBti0212991	91319

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTCCAAACATTGTTGCAGTGCTGTC	ATCTTCCTCTCTGAGCTTGGTTTCGG

Images

Construct Design Sequences

sgRNA sequence	CAGATTTACTGGTTTACAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGGAGCAATGTTCGTGTGA	AATTTATTGCGCACGCTCGT	TCTGCCATTAAGTAACACTTTGC	CTTTTCGTAACGCTGAGCCG

 

Amplicon 5'

Amplicon 3'

TCGGAGCAATGTTCGTGTGAAAGTCGAGACGAGAACGTAAACATTTTGCCTGCCTAGCTGTCTGTGCTGAGCACTTCAACTGGCCGCACAATAGCTCCTGAACCTAGCCCAGTTACACCCACGCTAATCGGTCTTCCTCGTTCTGGATTCCATTGCAGCATGAGCACAACCGCCGCCAGTCTCATCGAGGCGGCTCTAAATGCCAGTAGTGGAAACGCCACGGCTACAACTGCGGGATCGCTTGAGGCTACAGGGAAGCCCAACTACGTGGTGCAGGTAAAGAACACTCCGATGAGCACGCCGCTGGACCCGCTGCTCCACGTGGGCGATGGCATCTTCCTGCAGACAAAGACCGCCCAGGTTCTAGCTGGGGTGTGCGTCTGGGCCGCCCTCTTCATCACATGTCAACAGGTGAGTTAAGGCGTCATTGGTCTGAGTTGTGTACTAATGGATATCTTTGGCAGATCTACCAGCACCTGCGCTGGTACACGAATCCACAGGAGCAGCGCTGGATCGTGCGCATTTTGTTCATTGTGCCCATCTATGCCACATACTCCTGGATCAGTCTGCTTTTCTTCAACTCGGATAACGTTTACATCTACTTCTTTACGGTGCGCGACTGCTACGAAGGTTCGGCTGATTTGATTTTGAATGATTTTTGCCCATCGACTAATTGCTAACCCCATTTCCATCCAGCCTTTGTCATCTACAACTTCTTGTCGCTGTGCTATGAGTACCTGGGTGGCGAGGGCAATATTATGTCGGAGATTCGCGGCAAGCCCATCAAGACATCGTGCCTGTATGGCACCTGCTGTCTGAAGGGCAAGACCTACACGATTGGCTTCCTGCGCTTCTGCAAGCAGGCCACTCTGCAGTTCTGCCTGGTCAAGCCGCTGGTGGCCTTCATTATCATCTTTCTGCAGGCATTTGGCCACTACCACGATGGCGATTGGAGGTAATGTGGTTTATCTTTAATGACCCGCGTTATTGCATCTGAAATGGTGGCATAAAAACAACGAGCGTGCGCAATAAATT

TCTGCCATTAAGTAACACTTTGCAAAAGAGAGAACTTATAGGTACATATCATTCCCACTAATAATATCTCCTTATCTAAATTGCAGTGCTGATGGCGGTTACATTTACATCACGATTATCTACAACATCTCCGTGTCGCTGGCGTTGTATGGCCTCTATCTGTTCTACTTCGCCACGCGTGATCTGCTGACTCCGTTCGAGCCCGTGCTAAAGTTCTGTACCATTAAGTCCGTCATCTTCTTGTCCTTCTGGCAGGGCGTGGGTCTGGCCATCCTGGAGAAGGCCAATGTTATCTCGCCCATTGTAGACAGTGCAGGCACTGTTACCGTGGAACCCGGTACCGTCTCCGCCGGATACCAGAACTTCTTCATCTGCATCGAAATGCTGTTCGCCGCCATCGCGCTGCGCTATGCATTCCCCTATCAGGTGAGTTTTGGAATGTCGGAGTGGGCTAGATTTTACGCAGCTATTTACGATTTTTGGTGATTAGATAGTGCTGACTTGAGTGCTTTTTTTCGGTTATTTCGGTGTTTATTAAGCGGCTTTCCTTACACTTGTTAGATATGTGAAAGGTATTCGTGTAATGCTGACTTGGCTAGAAGAAATCAGCTAAAACGAAGTGCTTGCAGTAAAAATAACTTAAATGTTGTATGCTCTTTGTAGGTCTACGCACGCAGCTGCATCAGCGATGGCCACGGACGGTCTGTCACCATGCAGTCCATTTCCAGCAGTCTTAAGGTAAGCTGGAATATTTCCCTGGGAATTCCCGTATCTAAAACTCCCGCAATCCTTCGCAGGAAACAATGAATCCCAAGGATATTATGACAGATGCTATACACAACTTCCATCCGCAGTACCAGCAGTACACGCAGTACAGCTCAGGTTGGTTTTTACTCGCTTTCTCGTCTTTTTAGCCCAACTTCTTCTCTTTGTGCTCTGTGTCTGTGGCATGTTCGTTCTTTTCATCGAGCCTGCTTAACTTATCTTATCTCTTGTGTTCGTCGCTCTCATTTAATGCATTTCGATGCGAATTTCCATTACCATTTCCATTCCATTATCGTTATGTTTTTCACACTTTTGTTTTTCTTTCTTGTTTTCTTATCTTTTGCAGAAGTAACCTCGGCTCAGCGTTACGAAAAG

 

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