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CRIMIC CR01834

Gene ACXD

CG5712

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:2243719..2237608	62D3	FBti0212992	91320

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATACCATCTGGCGGTTCTTCTTTCC	GATCAGCAGCATGGTGAGCAGGATAA

Images

Construct Design Sequences

sgRNA sequence	CAGTAATCACAGCTTTCATCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTGGATCGGCAGTCACCTT	TGCGAGTTATCAGTGGTGAG	TGATAACGATTAGGAGGGAACTG	CGGAAGACTTTGGTGAGCCT

 

Amplicon 5'

Amplicon 3'

CTTGGATCGGCAGTCACCTTTTGCTACATAATAAACTACAGCTTCGTGATCACCGCCAAGGATGACAACCAAATGCACAGCATCCTGAACGAGGCCATATACCTGAGCTGTGTCAACCTACTGGGCATTTTCTTTCGCCTGATGAGGGATATAGCGCTACGCACCACCTTCCTCGATCGCCGGCAGTATGTGGAGGAGAATCTCCTGTTGCGCTATGCCAGGGATCAGGAGCGGAGTCTCTTGCTTAGCATCCTGCCTGCCCAGATCGCCGATCGGCTGCAGGAGGATGTGAAGAACCGCATCGAGCGATCCAAGCAGCAGCACCAGCAGCAGTCGCAGGTGGATCTGCGCAGGAGTGCGGATAGCCAGACCCTTAAAAGGTGGCGACAACCGGACCATGGGTGAGTTGGATGATCCCAGTTTAAATTACTGCATATATTACAACAATTCTGCCTTTCTCCAGCACCCTCTTCATTGAGCCCCACGAAGATGTCACCGTCCTCTACGCGGATGTGGTCAACTACACCCACTTGACCACCACCCTGGATGTGAAGAAGCTAGTGGAGGCTCTGCACGATCTCTTCGTGCGTTTCGACATTGCCAGCGAGGAGTACAACGTGCTGAGGATCAAGTTCCTGGGCGATTGCTACTACTGCGTGGCTGGGCTGGCCAATCCCAATGCGGATCACGCCAAGTGCTGCGTGGATCTGGGACTGCGTATGATCAAGGACATTCGAGATGTCAGGTGGGCACAGCTTCATGCTAAGCGTTCTTAAAATATGTATGTCATTGGCCGTGTTGTGATAACTCACCACTGATAACTCGCA

TGATAACGATTAGGAGGGAACTGTTCAATCGAATCAATCTATCATAATGGAATTAAAGGAGTTTGAGCTGTTAAGTATCAGGCATATATTTCTTTCATACTCAACTAGCGACTCAACTCGACTAAACTTTCCTTCTTCCTTTTCCCGAATTTAACCATTTCAACTGGAATTTTGTGCTGCATTGAACGTGATGTCATGATTGACCCACGTACTTTACCAAACTTGATAAGGCATACAAATTCAAATGGAAACCTAGTACCCAAAGGCAGCCCTATTAAATTGACCAATAAAAGTCGTTTGTTGGCTTGCTAATAGCTATTTATTGTGTGCTTTTGTGGAAAACAAAAAGTATATCCTTTCAATCCTTATTTTACTTTCTCCTTAGAGAGAAGCGCCATCTGAATATCGACATGCGAATCGGCGTCCACTCGGGCGATGTCCTATCCGGCGTAATTGGAGCCGCCAAGTGGCAGTTTGACATATGGTCGAAGGACGTGGATATTGCCAATCGCCTGGAGGCCACTGGAGCCACAGGACGAGTGCATGTGAGCCAGAAGACCCTATCCCTGCTGGATGGCGAGTACTTCTTCGAGGACGGCACCGAAAAGGCTCGAGAGGATCCGGTGCTGCAGAAGCACGGCATTCGCACCTTCCTTATCAAGTCACTGCGCGTGAGTATTCCCCGAACGGCCCTGCGTTCCTTGCCAGCACCATTATCTCGAACCGAAAGTTGTAGGCCCCCATGCATGACCCGCGTCGCAGGATGAGGGAGCGGCAGGTGAAGAAGCTGAGCGAGGCCAGCAAGGCCAACTTTATGCACAACTCCACGCTCCACCAGTACAACCAGGTGCGCAACCAGGCCAAGCTGGAGATGTGTCGCGAGCTGGACAAGATGCCCATCGGCAGGATACAGTGAGTAGTCCTGTGCAAGTCCTCAAAATGTAATGCAATACTTAAGGACGTCCTCTTTCCAGGCTCACCAAAGTCTTCCG

 

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