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CRIMIC CR01835

Gene BI-1

CG7188

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:8308941..8307145	66C5	FBti0212993	91321

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGTACCTGTAACTCTCCGCAGAGC	CTGCCTCCAAAATCCGTGTCAGATGT

Images

Construct Design Sequences

sgRNA sequence	CCGATGGACAACTGTTGATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGCTGCAGGAAATACCGTT	ACAGTTGTCCATCGGATCGA	GGTTGGATACCAAAACAACACGT	CAGCACCTAGTCGCCCAATT

 

Amplicon 5'

Amplicon 3'

TGGCTGCAGGAAATACCGTTTAAAATGGTTTTTCACATCGAATTTTATACTGGAAGCTTTTGCAAAGTGATCAGCATGTTTTCGAGGATAATCTGATTGAGCTATTTTGGGCAACTGGCTCCGGCTTGAGCTATCTTAAACCATGAAGGTCTTGAGCTGGGTAAGGGGGCTATATAACAACTGATCGTTGGGATAAACCCTTGTCGCGGAAACATACCTTTTGCGTCAGTATAATCAGCAAACGGCGGAACATGCTGAGTACATCGAAGAACAAATCTAAAGCGTGCTGCACCACATCTCGATTTCCGTTTCGGCACTTCTCCACAATGTTCTGTGTATCGTAGACGATGAAGGCAGCCATAACGAAAACGCCGACGTAAAGTTGAGTCTATAAGGAAAATAAGAATTTGTTAAGTATTACAATTATGAAATATTCAGTTTTCAATGCCTTGGAATGGAAATTAGAAATTAGATTTAGAGGATACAAATTAATACGCAAATTAATGGGCACTCACCACTTGCACGAAGTAGGACTTGAAGACCATGTTAAAGAGACTCAGCAGCGCCATGGTGTTGATGACGCTAACCAGCATCCCACCCAGATAGAGGTACTTGCCCTGCTCGGCCAGAAGGGCGGACAAGGAGAGCGAGATGAAGGTGACGAAGGTGCCCGTAAGGGCAGACAGGATTATTGCCGGATTTATGCTGCATATATAGCCGAGGAGCGGTCCGAGCGTCTGGCCGGAGCAGAATCCGAAGGCGTAGAGCATGCCCAAACGTGTATAATAGTTCTTGCCGTCATCCTTGTAGAAGTGCAGACCCAAGACCAGGACTAGAGTGGCCACCGCCGCCAGGACTCCAAGATCGAGAAAGTCACGCATCTGAAGCATGGCTCCCATGGCCGTGGCAGCGGCAGTGCTGCCCAGGACCATGTAAACCTTAGACAGGTGCTCGCGCACATAGGGCTCACTGAAGGAAATAAAAGGAGGACTATTAGTTTCAAGGTCCATCGATCCGATGGACAACTGT

GGTTGGATACCAAAACAACACGTCAGTGCAACGCACTTGGACCCCAACCTCTAAGGGGGTGACCCATCGGCATGTAATACTTACTAACGGTCACCGAGCCCGTTCATGAAGGTCTGGAAACGGTCGTTGATGTAATTCGCAGTATCTGCCATTGTAGTCTTTTTCGCACTTGCTCTTGCTAGTTTCCAGTAATATTACACATAAGTTTGTCAAACGGAGGAGTGAATTAGAACTGTTTGCACTGATGTTTTAAAACTGACTGTTTACTTTGTAAGGCCTACTCGGCTCTATTTTCTTGCCTTTTCTTTCTGTATTTCACGAAATCTGTTTTATCAGCTTTTAGTGTTGGATAAGTTGCTATAGGTTAGTGCTGACGACGTGGTCTTTTTACAGCTACATTTAGATTTGAACTTTATGCTCGTATTCATTATTAAACTCACCGTATACACATAAATACTTGTACAACATACTATAAATTAAGTTTAACATATAATCCTGTTAATTGAATAAATAAATAATACTCCAGTTCAATTTTGTATAAATCTTTTGGATTTTATACCAGCCTTTTTTTTTTCAAAACTAGAACACCGTCAGCACTGTTGATAAGGTGGTGTTTTCGACAATTGCCCACCTGAGTCAGTGTTGGGTAAACGGACATTTTTCGAACTTCCAGATGATTCTATGACGTGTTTTTCGGTTGCGCGCGCTGCCTACTTTCCATTTGAAAAGCAACGAAGTTCGAAACGACTAGTAAATGTTTTTTTTATTTCGCGCTAGATCCAAAAACAAGGTAATTGGCGTATTTTCTAAAAACATTGTACATGGTAAATAATAAACTATGGCAAATTGGGCGACTAGGTGCTG

 

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