• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01836

Gene CG7589

CG7589

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:17470127..17474517	74C3	FBti0213438	91420

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCTTCTTTCATGGCCTGCTTGATCC	GATGGGAATGCAATGTTGCCTTCCTG

Images

Construct Design Sequences

sgRNA sequence	CGGCAGAAGATATTCCATTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGAGTGGTTCGGTTGGGAG	GCCGTGAGTAAAGTTCTCATCG	ATCTTGCCAAATTTCCATCTCG	ATTTCACTCGGCTCCACTCG

 

Amplicon 5'

Amplicon 3'

TTGAGTGGTTCGGTTGGGAGTTGCGACTTGGGAGGAGAACCATCGTATCGTATCTGCCGGTCTTATCAGGCCCGAAAAAAGCCGAGAGCCTCTCCGCCCACAAGTCAGTGTGTTTTGGGCCGTCGGCAGAGGCAGTGGCAGAGGCAGTGGCAGTGGCAGTGGCAGCGGCAGAGGCAGAGCGTTACCTAGTATTTCTCAACCACTAGAGGGCGCAAACGCGGCGCGATCTCAAACCGCCGACGAACGCAGAGCGGACAGAGCGACTCGTCCTCGAGGGAGTTGCGGGAAATTCGTAAATAAAGTCGCCGATCGACGTGGATTTGCATTAATTGCAAAGTGAATGTGTGAAGTGAATGTGCGAGGAGATCGTGTGAAATATGAGCATATAATTTTACAAGCCAACGAAATCGAATCATTTCGCTAGCGGCCAGCCAAAGTAAAAGTTGTAAACCTCCCACCAAATCAAGAAACACTGTTTAAATTGCAAAAAAAAAAAACAAATAAAAAAAGAATCAAGCCACATGATGAATCTGCATCTGAGTGAAATCTTTTGGATCACGCTGATCTTGGTCCTCAGCATATGGACTTCAGTTGGGTAAGTTTTGACCGGGAAACATCCAGCACGCAAAACATCTTACAAATCATTCAACCTTCAGCAATGCTCCGATAAAGATACATTTTGGTGGAACTGTCTAAATTTAGATAATAATTTGCTCTCACGTTCATTTTTTTTTTTTCGCCGTAAGTGGAAGTAACTTTCCCAAAAATTATCAAACAGTGCACCTTCGTTCGATGAGAACTTTACTCACGGC

ATCTTGCCAAATTTCCATCTCGTATTTGTTTAGAAAGCCACTGAGAAAGGCTTTAAACTAAACACGCAATTAAACATAAGCATAAGATGTCAACAGATATAGTCGAAATGAATAAACAACATTTCGTCGAAAATTCCCATCTAGATCATTTTGATAGGGGTGCTTCAGAAAGTATGTCTGATAACGTTTCCCAAAGAGATTCAAGTGAAAATGTTTACATGGAGGCTGATAAAAAAAAGGGTTACGCATGAAAAATGTGGTAACTTTTCATTTTAACATTTCCTGTAACCTGTTCCCTTGGGAAATTTCAGATGCGCGTGTTTTTTTCAGGTCTTCTATTCTTATGCTTTCGGAATTCCCCCAAAGCCCCTTAAAAATCTTCACCGAATTGCGTAAACAAATCTTTAGATTAGAATTTAATTAAGATTTTCTCGAAAAGTATTTACAGTCGGCACTACTCCTTTGATGTGCGAAAAAGGTCGCGAAACGGTGAAATTTTCTTATCGGATAAGTTCAAGATCTAAGCGCTTTATCAACAGGGCTATAATTAGTTTCCTGCTCTGATGGGACGGGACCAGGTCAACTCGATTAATTGAATTAAAACATTTTGTTGTTGCGCAAGCAAGCCAACACATACAAACAACGAAGTAACGAAATAAAATGCAAGTTAAATAACGAGCGCCATATGAGGAGACAAATATTTTGCTATTATTTATTTTTATTCAGCCATTTATTCTGCTGTTTGACATTTTAAGGTAGCTGCAAATGAGCTTGCACAGCTGCTTAAAGGTGCTCACTGCTCAGTCATGCTAACTCCCCCATTAAAAAATCGAGTGGAGCCGAGTGAAAT

 

---

Search Again