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CRIMIC CR01839

Gene CG6878

CG6878

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:15162470..15161817	71B6	FBti0212994	91322

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCGGACCACTTAAGAGAACAGCTCT	CAGGTGGGTGCAGGTGTACAATTGAT

Images

Construct Design Sequences

sgRNA sequence	GAAGTGACGCAAGTGGAGGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTATCGCGTGACGGAGAAT	TTTGGAGGAGCGCTAAGTGG	ACTCCGGCTAACTAACGAACC	CACTGCATAATCTGGCGTGC

 

Amplicon 5'

Amplicon 3'

GGTATCGCGTGACGGAGAATTGATTCGTGTATATGGGCTCGCCGTCGCTGTTTCCTCGCATATATAATGTGGGAACAATTTTTAAATAGTAATTGAACATTTCACTTTTGGTTTCTGCAAAAAGAGGAGAGCAGATTTTCTAGTTTACTTCATTTGTCGTTGATAAATGTATTTAATTTGGTAAGGTAATTACGAATTAAACTTCATGCTTCTAAAAACAGGTATTTCACAATTCGAACAAATGAGTATATTTTTTCCAATTTAAAAAATGAAAATGCCCCACACAACAGACCTCCGAAGTTTCCCAGAAATTCTTCGAGGAGTAAAATATAAATTAAAATGGATGCCTGTGCTTAAATAACTTAGTTTTTTTTTATTTCAGTCTTAAGTATGCTTTTTAATAATATTAATAAATAAAGAATATAAATTGATTTTAAAGCACCCGCTTTTTAGCTACCGCCCCCTATCGGCTTTCTCTAGAACGAGGCAAGCCGAGCGAAAAACAAAGTGGCAACCCTTCTATAATTTGACAGCTCACGCACACCATCACTCAGCTGCTCACACTCACACGTTTACGAATTATTCGACTAGTCGGTGCCGAAATAAATAGAAATATTTTGAATATTCCTAAAATAAACGAACCTTCTGCGAGATTCATAAGTGCGTCGGAGCAGCGGAGCTTGCTACTCAGCGGTATCAACTCATATAGAGCCGGTGAACCAGGAAAACCACGGACTTAGCGGCATGCCTCTGCCTACGAGCTCATTTTCCCAGCAGGGACCAACGTGCTTCGATAAGATGAAGACGGGCTTCATCATCGGATTCTGCGTGGGCATGGCCAGCGGTGCGGTCTTTGGCGGGTTCTCGGCGCTCAGGTTAGTTGGACTTTGCACTCGGATGCTCCACTTAGCGCTCCTCCAAA

ACTCCGGCTAACTAACGAACCACATTGAAATACACTTTAGATACGGACTACGCGGGCGGGAGCTGATCAACAACGTGGGCAAGACCATGGTCCAGGGCGGCGGAACCTTTGGCACCTTTATGGCCATCGGCACGGGGATTCGCTGCTGATCCTGCTGGCATTTGACTAGAGTTACTAAACAACCACAACCTTAAGCCCTACTCCAATCTTGTTTAAATTACGCTAAGTTATTGCAATAGAGATGTTACAGAATTACGAGTCCGTGTTTGTGTTCACTACTCACCAGCCACGTCGACTCGTAGTCCATCCAAGGGATGCGTCTTGGCGAACTCTATCTTCTCGCCGAACGATAGGTGATTGATTGTGTGCGATAGCTTCACATTGCTGAACTGAAAGTCATGGATGTGGAACTGGCGGATGGAGAAGCTCTTGCCGGGCGCAAAGTGAAAGCTGCCGGCCATTCGATTCACTTCCAGGTGACCCTGGATGCGACAGCCCTCCTTGAAGGCGTCCTCGTCGCTGCGTTTGTACTTGCCCTTACATTGCTCGATCTTGTCCACGGCCACGGTCCATTTGCGCAGCCGGTAGGCGTCGAGAACATCCTCGCACGTGTTGCAGCAGCTAGATAGGATGGTAGTTGGTATTAAAAGGGGGAGATTAGAAAGCCATTGCTATAATTAGCTCTAGATACAGATATCTCCTTAGATAAATCTTGTCAGCGAAAATAGGTGGAACTAATGGTTGAACTTAGAAGCTGTTCACTATCTTACTGAGTAGCATTGTGCTCCGCTCCGTAACAACTGCCGCAAGTGACATTCTTATTGGGCGGCGAAACTGCAACGATTTCCTTGATGGGCGTCTCCTTGAGGGGCTCGCCATTGAGATCCAGCCGGTGCTTAAAGACGTCGTGGTCCACCCTCAAGTGTGTGTCGCCAGATGAATCCATTGCGTCCAATGAGATGTAGTTGCACGCCAGATTATGCAGTG

 

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