• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01840

Gene MsR2

CG43745

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:2300944..2282883	62D5	FBti0212995	91323

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCGCTGGACCTTTCCAAGTGACTT	CAGGAGCACATTCAGCAGACCCATAA

Images

Construct Design Sequences

sgRNA sequence	GAAGTGTGCCTAATGTGGATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACCAAGAAATGCGTGAGTGT	AGGCACACTTCGTTAGCCTT	GATGGGCCCCTTTCGGATTA	GCAGTTCCATTGGCCGAATC

 

Amplicon 5'

Amplicon 3'

CACCAAGAAATGCGTGAGTGTTACACTCACAAAATATGTTCTATTCCCAAGAAATTCTTCTCTGTAAGTAAGTGTAAAAGTGTGTACACTAACTCTGTTTGTATTAAAGACAAATGCATAAGAATGCCTTAAATTCGTCTACGTGTAACATGTAAGCCAGAATTTTTGTTGCATGTTACACGTTTTCCCACAAATTGCCAAAATCATCTGCTTCTGCCGGCCCACTAGTCGTATGCGTATTATTGATTACGGAACGAGGCCTTGTTTTCTTACCATTTCGGACAAATGGTAATGACATTTCATTGTTGATTCGTTTGAAATCGTACCTTTGACCTGCCCTGAACTTGGACATCGTTCTTTGCATGTGGCATGCAATGCCATTGTATTTGGGGAATTTCGAAGCTTAAATTCTGGTAAAATTCAAACCACAACACTGAATTCGTTTTTTATTCACCTAACGCCTGATGTTTATTTGCAGGTAGCAGGTAGCACTTTCGCCACGCAGCCTGCATATTTGAGTGTTGGAATTTCCGAAAAGGGAATTTAAATGAAACGCCAGAGGAGCAGCGGGGCGCACTGAGTTGGCAGCCGGACACCGCGGTCAATCAAACGGAGTCCTTGCAGGACATCCCACCACCGACTACCATCTGGCCTTCATTGACACCATGGTCACGAACATGTCGCAGCCGCATTATTGCGGCACCGGCATCGATGATTTCCACACAAAGTGAGTAGAACGAGGACGTGGACTTAGGCCTGGGCACATAGCCCCTGGGTATGTCTGCATATTAATTAGTTTTTTAAGTGTAATTTACATAATGATTTGCCAGCCGGGGGGCGCATAATGAAATCATGTGTGTAATTGCCTACGGCCGTGGTGGCAAAAAGGCTAACGAAGTGTGCCT

GATGGGCCCCTTTCGGATTAAACTGAACAAATACTCGCATAAATCCATAAAGCAGATGTTAAAGGTCGGAGATGAACATTTTGAAAAATATATTGAGTTCAATCTAACCAACCATGTTGTTTATTCGGTTTCTTTCTCTACATAAGCTCTAATTACTTTAAGAATTTAATCATACCCAATTTTGTGCTCAAGTGAAACTGGTTTTAAATATTTATCCCAATTTTTTTTGTCTTTCTCCCCAACAGCTACAAATACTTTCACGGTTACTTCTCGCTGATTGTCTGTATCCTGGGAACCATTGCGAATACCCTAAATATCATAGTGCTGACCCGACGGGAGATGCGCTCCCCCACAAATGCCATACTCACGGGTCTGGCTGTCGCCGATCTGGCTGTGATGCTGGAATACATACCCTATACGGTGCACGACTATATCCTCAGTGTAAGGCTGCCGCGAGAGGAGCAGCTCAGCTACAGCTGGGCGTGCTTCATCAAGTTTCACTCGGTATTTCCCCAGGTGCTGCACACCATCTCCATTTGGCTAACGGTGACGCTGGCAGTTTGGCGGTACATAGCGGTAAGCTATCCGCAAAGGAATCGCATCTGGTGTGGAATGCGAACTACTCTGATCACCATAGCCACGGCCTATGTTGTCTGTGTCCTGGTAGTGTCACCTTGGCTGTACCTAGTCACAGCCATTGCCAAGTTCCTAGAGACTTTGGATGCCAATGGCAAGACGATTGCCTCAGTGCCATTGAGTCAATACATTCTGGACTACAATCGGCAGGATGAGGTGACCATGCAGGTCATGTCGAGTACAACGCCAGATGTTTCCTGGGCGATACCAAGTGATTCGGCCAATGGAACTGC

 

---

Search Again