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CRIMIC CR01842

Gene CG7945

CG7945

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:15524137..15521711	71D4	FBti0212996	91324

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAGACACGGTCATACAAATCCCAC	AGCAGTCGCAGCATGATCATCGACAA

Images

Construct Design Sequences

sgRNA sequence	GAGAGTTGTGTGGGCGTTCGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGAACTTCTTGTCGACCGG	ACGCCCACACAACTCTCAA	TCGCGGCAGATACTTTAAGCT	ATCGGGCCAAAGGTGCATTA

 

Amplicon 5'

Amplicon 3'

TCGAACTTCTTGTCGACCGGCCCAACATCGGCCTCGGGCACGGTGTCCATGTGTCCGGATGGATCCATTGAGCTGCTGCAGCAGGCGTTTAAGTAAAACTGGCATCGTTGGCGTCCGATCACGGGATCACCCATCTTGATCATGCTGTCAATCAATACATTTATCTGGCTAAGGGAATCCTCCTGAGAGTTGTCCCGAACAGTGCGCACACGCAGCTCCACGGTGGCCAAGCGAGAGCTCACCCGTTGTAGGTACAAAATGATCTCCTCTCGTTCAGCTGCAAAAGAAGGTGGTACAACATTAGCTCACAGTCAACTATGAATAACCCTTTACTTAGTTTGTCATCAGAAACCATTACTTCCGACTTCGAAATAATACACATTTTAGTAAAGCTATGAACAATGAGGGTTCAAATTAGTTTTAAGAATCACTGTCACTTACCTTCGCTCATGTTCTGCATCATTTCGTTGCTCTTGATCAGATCCATGGACATCAGAAGGTAGTCTTTCTTCTCCTGCAGGTTGAGTGCATCCTTGCGCAGCTTCTCCACACGTGCATCCAAACTATCCAGTATGGTGACAAATCTGCGAGTCACTCAAAGGTTAGTCACGCTCAGTGGTTATTATAAATTCAAGATTTACCTCTCGCTGGCGTTAAATGGTCTGTCACATGTCGGTTAAGTGTGATTGCGGTTCAGTGGTTAAGTCATGAAACGAGACAAGCAACGTTAGATTGTTAGATCGCTCTGCTACAGATCGACTGACCACTTATAGAGCTACGAAATTATAACCAGAATATGTCTACAAGAAAAGGCGCTCGCACACAAAGCTCTTTTGAGAGTTGTGTGGGCGT

TCGCGGCAGATACTTTAAGCTATCACCATTACTATTATGGAACAAAAGATTCGAATGAAGCTGGTAGCTGGTAGTGTGAAGAAATAATAGATAAGAAACGTATAACTTTTGTTTCGCTACGAGGTTGTGTGAATATAATCGGTGGTATTGTTTTGGTTTCGTGTCTGATTTGGTTTTAGTTATGATGGAATAACAACCTGTCCAGAGCTCTACTATCGTCGACGAGTCCGTAGGAGCTGTTCCCGCCATTGCCACCACCTCCGGATCCGGATGGGGATGAGGAGGCCATGGCATCGGCCCTCAGGTCTTGCAAATGATCAAAATTCATGGATGCCTCCGAGTGGTAATCGCTGGGCGAGATCTGATGCAGCTGCTGGTGATAAGTACTATGGTGATGATGATGAGTGTCACTGGTTTCCTGCGTTGTTGGCGCATCAACTTCCATATCCTGCGGCTGCTCCAGATTTGATTTAAACCTGTCGCCCTCCAAAAAGCCGGTCAAGTGTTTGTCACCCAAGATGGTTGGATAGCTGCCTACTGTTTCGTTTTGGGTTTTGGTTTTATCTTATTTATTTTTTTTTAGCCGCTTTGCTGGCTACTTTTTTTGGAATGTCTTAAGCTTTTTTTTTCGAGTGGGTGTCGAAGTGTTTGATGCTGTTGCTTAAGCTTAATTTTATCTGTTTTTGCTTTGAAGCTGCAATAAGAGAGAAGAGGAAGCGTGAATGCGCAGAATTCAATTACCTATCTGAATGTGGGCTGTGAAAAGAGCCTTCGGTCCAGGAGGCGTCAAAGCCATCATTGGACTCGGGCGTATCGGCTTCTATATCTTCCGGATATCGGTTTATTATCTCCGCCTCCTGGGCGGGTTGCGGCGGCGGTGGCGGCGGTGTTGTGTCCGACTCGCAGCGACGCCGTTTCCGAAAAATACCTCGAAAATTCTCAAACATTCTCGGCTGAAATCACCAATTTTCTAGAAGATTACGCCTAATTCTTCTTCTTTTCTTGTGGCAACCGATGTCTAGGAGAATCCCCGGGTGGCTGTTTTGAACTTGAATAAATTACCTGCCGCCCGAGACTTGAGGCTCCGGTTTTAATGCACCTTTGGCCCGAT

 

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