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CRIMIC CR01843

Gene CG7879

CG7879

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:1568608..1559813	62A5-62A6	FBti0212997	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAATCCGACAACGAGCCAATCGCAT	CTATCGCTATCATCGGAACTGGACGA

Images

Construct Design Sequences

sgRNA sequence	GCCCCGACCCAAGTCCAGGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCCTTCAGCATTGCGCAG	GCCAGAGACGCTCCTAACTT	CTGGAGCTCCGAGATTGCTA	ACTGCCATGCGAATCCATGA

 

Amplicon 5'

Amplicon 3'

GATCCTTCAGCATTGCGCAGCGGGCATCCTCGTCGGTGCTATTGGTTTGTAGAAATTGGGGAAAAAAAAATCAAAGGCGAAAAATCAGGGGTGTTACATAATAAGGGTGAGGGCAATATCCCGAGCAGTTTTCGAGAATTCGGCGTGTTTAATGGCCCCATATTTGTCATCAGCCAGCATTTTAGGCAGTCAAAATAATAGCACTTTATGTATATTTATTCCATATGTTATCTGCATCATCATCCACGTTCCTGGTGGAACCATTCTTGATTTGCCATAACATGAACCTAGGATCACAGAACTTGAACTCAATTTACCCGATTTAATCTATTTAAAACCAAACAACTAATTTCAAATCATCAGCGAATTCCCGATAAAAGCACAAAGGTAAAAGGAAATGAATCCTTATCTTTACACAGCCCACTAAATGTAATATAAAGATCAAAACTAAGGTCTGCTAAAACTGCACGCTAAAATCGTTGGCGATTTGAAACCTTTTTACATTCTGAAGTTACGGTGGTCCCATTATAATTCGAAAATATTTCGGTTTCATATACATTGACGTACTTACTTCGGTTACTTCCAATGCCAAACATATATTTAAAAAAAATGATCCACCAAAAATAATAACATTTTCCGATTTTGCCGCTTTCTGAATTTAAATCTATCGATATTTGAAATATATATGAAATCATAGGGTTTTTGAGATATGCATTCTGGAATGCTTATTATGGAATCCGAGTGATAAAATGTGCCATAACTAGCCCGATGAGATAACTCTAAATGGAATGAAATAGCCGAGGGGCTCGTTATTACCTGTTATTTCAGTCCATAAAACCATGAGCTATGCAAGTATTTTCATGTGAACTTTCTTAGTGCTATTACTTTGACCACCGCCTTGGGTACATGCAAGTTAGGAGCGTCTCTGGC

CTGGAGCTCCGAGATTGCTAGGGGTTTACTTATAGATCAGTTTGTGGCTAACTACCTGAAAGCGATGAAAGCGTCGCCCATCTCGCCGCCGATTATGTGGACACCGCCCTCGGGAATTGAGAGGCCCGAGAAGAAGTTTCGAATGTCGCGCGCATTTGCCGTCCACGGCAAATTTTGTAACCGAATAATCACACTCATCTTGATCAATCAATTGCTTAGCTTTGATTACTTATTTATTTCGTTTCTAGTACAGACTGTCTTCACTTGCGCTCCACTTCCATTGACTGGTCGCTGGCTCCTCCGTTGGACATCAGACGCGTATTGACCTGATATTGATGATACTGATGCCGCTGCCCGTGGATTCTTAAATCTGTAACGATAATCGCATACTTATGGAGGATTTACCAACAAAATGAAATGTCTTAAAATAAAGATTTCAGCTCAGTTTAAGGAATAAAAGAGAACAGACCGCTGCAATCCATTATTGATGAATCACTTAACATTCATTGTATACCCTTCAGCTAATCTTCCAATATAAGATGCTTATGCACTATCAGCGGATCACACAAGTTTTAACCAAAATCTTTCGGAAGAATTTAGATTAAACAATGACTTTAATAAAAACTACAGAAGACCAATAAGATGATACCCTGTAGGCGCCTGGCAATTCTGTATTTCCTACATTACTACTTTCCAACTTCCTTGGGGGAAAAGCTGTGCTCTGCTCCTGATTAAATCTCGAGTTGATCGCTCGGTTGCACACACATTAGACCCACTATTTCATGGATTCGCATGGCAGT

 

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