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CRIMIC CR01844

Gene eRF1

CG5605

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:20348355..20353873	77B4-77B5	FBti0213439	91421

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATTGCACGCAGACAGCGAGCATCAT	TTGTCGTTCGTGATGAAGAGCTGGGT

Images

Construct Design Sequences

sgRNA sequence	GCTTCACACCGCTTTAGAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAATTACGGTGTTCGCGCTC	TAAGGACCGGGTCAAGGGAG	TCAGCAAATCGAGTGACCCC	TGTGACTATTGTGCCGCAGT

 

Amplicon 5'

Amplicon 3'

GAATTACGGTGTTCGCGCTCTGCTTGTGCTTTCCACTCCACTCCCCTTCTTAACTTCCCCCCTCCCAGCCTATTTACTCTGTGTGCATGTGTAAATGAATACTTTAAAACGTTTTTAATCGTTGCAGTGTATTCATCGCCAGCCACGTTAAAAGGAAGAACGTGTATGTGAATACGACCATCAGAGGATCTTAGCGAAGGATTCAGGAGCCCAAATTCTTAATCCCCATCCCACACACACACATATACTCGCACGTAGGCACGCTCTCTGTGAGAGGAAGGGTAAATAAAAGAAGCAACGAGCAAAATGTCTGGCGAGGAAACGTCTGCCGATCGCAATGTCGAGATCTGGAAAATCAAGAAGCTCATCAAGAGCCTGGAAATGGCCCGCGGGTGAGTCTTATCAATGATATACATGTCCTTTAACGTCCCCCCCAACGACTGTTTTGCGTACGTGTGTGGGGTATACACAATGCATGGCACGAAAACAACAACACTGGGGTGGAAGTGGGCGTACTGGACGTGGGTGTGGGTGGGGACTTGAGGGAGCGGCGAGTACAACCACCTTTCCTTGTTGTCGCCGTGTGAATCTCCTTTGCATTGTTTCCAATCTTGGCAAGATCCCCCGCCTCACTCTATCTCTCTTTTTTGCATACGAGCAACACGTTTTTGTCGAGTTTTTATCAGTTCAAACTAGACTGATTGAGAGACGGAGAGAGAGAGAGAGAGAGAGGAGTGAGTGAGTGAGTGAGTAATCGCCTCGTGGCTGTTGTGCGTATGCGTGCATGTGTGTGTGTGTTTTTCCCCCAAAATGGGCCAAGCTTTGTGGACCCCTCGCTCTCACTCATCCTGCCTCGCTCACTCCCTTGACCCGGTCCTTA

TCAGCAAATCGAGTGACCCCCACAGGAGCATATCTGCTATGTATATACATATATATGTATATTGTTGTCAATATGCTCCACAATTGGAGCTAACATTACACCTCTTCCAATTGGAGTTGGCCACTGTTGCCAATTGGGATTCGCTTTGACAGATATCTTCCTATTGGTTTTCAGCCATATGCGAAATATATACACACATATGTATTTGTTTTTCTTTTTTTAAGTTCTGTGCTATTTTTTGTTGTATTTTGCATTCCAACATTTTGTACAAATTACGATTGGCACTCCTCTGTATTATAACGAACGAGAAAATGATTTGCATCAGAGAAACAAACGTTTGGAAGTTACAGAAACGTATTTGTTTCAATAAATTAATCAAAGTTTAAATTTAGTCCAATAACCGAATGTCAAGTTTAAAAGACATATGCCACGAAATTTCACGGCTAATTAAATAACTTTAAAGTAGGTTGCTATTATGTGCATCTGACGCGCAAATATACGATTGAATAGCACTGTTGCTATGGGAGAAAGAGCCATTTCCGCGACTTATGTTCTCCCTTGTTTGGGTTTCTTGCAGCAATGGAACCAGCATGATTTCTTTGATTATTCCGCCAAAGGATCAAATCTCGCGCGTCAGCAAGATGTTGGCCGATGAGTTTGGAACGGCGTCGAACATCAAGTCGCGTGTAAATCGGTTGTCCGTCCTCGGTGCCATTACGTCGGTACAGCACAGACTCAAATTATACACCAAAGGTAAGCTGAGCTAAGAAATTGTTCATAAACAATCGAACAAACGAACTCTAAACCGTATCACCAACCGCTTCAATTCAGTGCCTCCCAACGGTTTGGTCATCTACTGCGGCACAATAGTCACA

 

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