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CRIMIC CR01846

Gene Ir64a

CG10633

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:5382821..5378786	64C15	FBti0212998	91325

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTCTCTACGAAAGCGAGCTCTTTG	CGATCGGAGTGGAATCTTCATACTCC

Images

Construct Design Sequences

sgRNA sequence	TGACTGTGGCGATATTATTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAAGGACGACTCGACCAAC	TCGCCACAGTCAAAGGACAA	GGAAGCTTTGGAGGTGCTTG	TTGTTCCAAGTGCTTGCGTC

 

Amplicon 5'

Amplicon 3'

ACAAGGACGACTCGACCAACTGCGACCGGCCACATCGATCCATTTGTGCCGCTCCAGCCAGTAGAATATCGGTGCTCCGCCAATGTCCGTCTCGTTGCGTATGAGTGCCCCAATCATGCCATCAAACCGGCCGTTCTTCACATATCCCCACGAAGTCGTCCTGGACAGGACGAACGTCCAGTTGAACATGTCGCGAACCGCCATCAGCAGATTGAAGTTGAAGCGGTTCATCGTGTCGATGTGACCGGACATAGTGTGCGTGAGGTAGTACATTAGGGTGCCTGTCATGTTCTTGTTGGTCACCTGGCAAATGGACAGATATCGAAAATATTGCTGCACTGTAATAAACTAGTACTTTATACGCACTAAAACCTGTAAAGGTGTCTATGCTACCTAATATTTTCCATCCAATTCTTTGCGAAGTTCAAACACTTTTGTAGTTGATTCAAAAACCAATGTGATTTCTGTGGTGCCCCTTCATTTTTCTCGAATGCTCCACTGACCACCCCCCGGCATGATGATGGATCTACGCATCCCACCCAATTTCCCAATCCTCACCACAACCATGCATCGCACCCTGGCGTGCTGCATGTCCATCCTGCGACGAACTGTGGACGTGGTCCGAAACCATGTATGCAACTGGATTCCCTCCGTCGGCGTGAAGCTGCCAATTTCGGTCAGATTGACGTGGCCGCCGTAGTTGAGTCCGGGGTTCCTAAGCGATGGTCCAAAATGGGAGCAAGTCGTTGTGGGAATTAGCAGCCGGGAAGTCGACAGTTGGGAAATTATGTCTGATGTGTTGATGGCAACCAGGCAGAAGTGCCACGCTTCTCTCTGGTATTTCAGTGGAATGGAATGAATGGTTTTGCGGTCAAGTGTTGGTGAAATGATAGTTTGTCCTTTGACTGTGGCGA

GGAAGCTTTGGAGGTGCTTGAGGTCAATATGGTTGAACAGGGATGTGAGATACATTGATCTGGTTGGTAGCCATTGCATAATTAAATAGGCAAATATTTCTTTTTATTACAAAATGTCCTAATAATCAAGTATTCGAAATGAACAGAATACAGAATACATTTAATGACTATAATTAAATGCCTCCGAAACCGCAATTGCCAGGTTAATTTGAACTAACCACACATTGCTAAATGAAACACCAAATTAAATACATTGAATGTGTAAATAAATAAAATTTAAGAAACAATATAAGGTACAATGAAATAAGCAAGCAAATATATCAATATCAATCAATATATACAACAAGTCCTTTCAAGTGCTCAGTCATCAATGAACAATAAAGAGACAAATATGGCATAAAGTATTTTCGGTAGAGGACAACTTGATTCAAGTTAAGATTGAAGCTTGAGGTGATTCTGAAGTGGATGACCAGGCCAGTTAATCACTCACCAAACATCGAACAGAGTGTAGTGGGCTGCCTCGGACATTCGCTTGGCCAGAGTGAGTTCGGTGTTCATATACAGGTTCATGCCCCCCAAGAAGCTCTCGATGGAGGGCAGGTCCTCATCGTCGGTGGCCTGAATTTGCTGCCCGGGCGGCTCTTCTTCCTCCATCTCGCCATCATCACGTTTCCCCGATCCTTCGGCTGTCTGCGTCCTTCCATGCCAGGCGAAATCCTCTATCAATAGCCAGTGAAACTTCTCGCTGTAGAGCATCTTGGCGGAAGCCTTTGAAATGATTGTACTTGAGTACTTAATAAGGAGTCTACAACTAGACGCAAGCACTTGGAACAA

 

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