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CRIMIC CR01847

Gene TrpRS-m

CG7441

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:17852453..17849727	75A4	FBti0212999	91326

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GCACTTCCCAATCTCTGCCAGATAGT	ATGAACTCACTGTCTCCTGCCGTAGA

Images

Construct Design Sequences

sgRNA sequence	TTTAAGTCTGATAAGCAACTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGAACGCTCAGTGTCAAAA	TCTTGCATTCCCGGCTTACA	AGACTTAAATGCTTTGGTTCAGC	TGCTTGTAAGAGCCACCCTG

 

Amplicon 5'

Amplicon 3'

GCGAACGCTCAGTGTCAAAATTTCGGCCGTTTCGAACAAATAATTTATTTTTGGGCTTTGTTCACACATTTAGCACCGCATTATTCCCAATATTTTTTACTCAAAAGTTTTGGGGTACAAAATTCATAGCTCATTGCAATGTTCCGATTTGGCAAGCTCCATCGCAGTCTGTCTAAGGTGGCCAAAAAGGGACAGACAGCTGTGTATTCCCAGCGCCGTTGCATTCAGGCGAGGAGTTTCTTGCCGGGATTGGCTAGTAGGACGGCTGGAACGCCCTCTGCGACGGCCCAGGTAACCGGTGGAAACAAGCAGCACGTACACCCCCATGGTAATTCCGTTAACAGTGGCCACGAAGAAGTAAGTGCATTCGTAGAAATAGTTTACATAACACAAGACTCTAATATATTACCCCAACCCAGCATAACACCCGCTGGCCGAGGAAAGTCTTTAGTGGCATCCAGCCGACGGGTTCCCTGCATCTGGGTAATTATCTCGGCGCCGTGCGCAAATGGGTGCAGCTGCAGAATGCTCGTGACGATGTCACCGTGTGTATTGTGGATTTGCACTCTATTACCATGCCCCACAATCCGCCGCTTCTGCGAGAGAACATCTTCACCATGGCCGCCACGTTGTTGGCATGTGGCATCGATCCCACCAAGAGCACCCTCTTTGTCCAGTCCGCCGTTGCCGAGCACGCCGAGTTCAATTGGATCCTTAGCTCCTTGACCACCATGCCGCGATTGGCTCAACTGCCGCAGTTCCGAGAGAAGTCCCGTCTTCTAAAGGATGTCCCTCTGGGATTGTACGTTTACCCGGTTCTCCAAGCAGCCGACATTATGCTTTACAAGTAAGTAAATATATTCCGTTTTTTGTTCAGAATGTAAGCCGGGAATGCAAGA

AGACTTAAATGCTTTGGTTCAGCTTTACATATTTTTAAACCACCCTTTAATATCTGAAATGGTTTGCCATTTGCTTACTTATTATAGTACTTATAATATTCATATTCGTTTTTCGATCAGGTCCACTCATGTTCCTGTGGGCGCTGACCAGATTCAGCACATTCAGCTGGCCCAGCACTTGGCCAGAATTTATAACGGTCGCTATGGCGAGACTTTCCCTGTTTGCACCGCCATCATTGAGGATGGTGATGCCTCCCGTGTGCTGTCCCTGCGAGATCCCTCCAAAAAGATGTCTAAATCGGAAGCGAATCCCAAGGCCACCATCAACTTGTGTGACTCGCCCGACTTGATTACGCAGAAGATAAAGAAAGCCGTCACAGACTTTACATCGGACATTACTTATAATCCTGGCAAGCGTGCTGGCGTCAGCAATCTGGTCAACATCCATGCCCAGGTCACGGGACAGAGCATTAAGACAGTGGTGAACGAGGCCGCCACTTTGGACACGGCAAAGTATAAGGACCGTGTGGCGGAGGCTGTGGTCGAGCACTTGCGTCCGATCCGAGAACAGATACACCATCACATGACTAAGCGCAACGAGATGATCTACTTGCTGGAAGTGGGTGCAGAAAAGGCACGTCAGCAGGCACGCCAAACGTTGAACGATGTTAAACAGCGCCTAGGACTAGGAACCAGTGCCAACATTCCGGCTGCGGTGCATGTGGCTCCACTTCTGCCCGCACCCGATATTTCCAAAACCTCAGCAAGCAGGCGAATGTCCAAGGACTTCGACGGGAATGTCCACGAGCGTCACGAACGGATGTACGGATTCGGAGATCAGCCAAGCGGAGGAGCTGGTCAGTCAGCCACCGGCGAGGTGGCGGATATGCCTCAGGCCGTTGTACCGCGGGTGATCCGTCGGGCGCCCATCGTTCCCACTCAGTCTATGCGCGTAGAGAAACAACTAGGTAGCACTCGAACTCGGCACGTATTCCAGATGGACACCTGCAATGCTCCGCATATAGGGTTCAAACCGCCGCCAGGATTTTCGGCCAGCATGGTCACCGGTGCTCTAGCCAAGAACAAGCGCGCTACAGTTAAACCCGTTACTGGCAACCTGGGCTTCGGCCAGCAATCGCAGGGTGGCTCTTACAAGCA

 

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