• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01848

Gene Thd1

CG1981

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	4:557854..538332	102C4-102C5	FBti0213000	91327

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCAGAACGCTCTCTCCAAGCAACA	GTTTGCTGGCTTTAGGGCGTTAAGGT

Images

Construct Design Sequences

sgRNA sequence	AGATTGAACACCGAGATCACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTCGGTTTCGCGCTATACA	AATCTGGGAAGCAGTCGCA	GGAGGCTGCTACGGATCATT	AGCTCACACTGACCCCAATG

 

Amplicon 5'

Amplicon 3'

TGTCGGTTTCGCGCTATACAGATTTTAGGCAACTAACGCTGAAATACTTACTGTGTCCGTGCCATCAACACGATCAGGTTGGCGACCAAAGTGAAATTCCTTTTTTCCCGAAAACACTTCAAATATTAGTTTGCCATTAAAAACGGCTACTTTTGGGCGAAACCTCTGAAGTTTTTCTAGCAAAATTCGGCTGCCCTCTTTTATTTCCTTTCTTGTAAGGTCAGCAGATCCTTTCGTGGCTCGAGCAACCATATTGGTAAATCCTATTCCTTGCTTTATCAGCTTGTGATCTTCATCAGCACTCATCTGCTCCTGAGTAAGTCCTGCTAAGTAAAGACACTTCCAGAAGTGATTGCCTGGACCTGCGTAGTGGTGTCCCTTGTATGCTGCAAATAACCCAGGATTTATCCCGACCTGTAAAGCGCACATGCAAAAAGTTATTAACATTGTTCTTTGAATATAAATAGTGTTTTTTTGTATTTTTAAAACCGAAATTTACAACTCACTATAACGATATCAAGGTTATCACATAGATGATCTGGAATTGTCCGTTTAATCACTTCCTCCTCGGACATTCCATTAAATCTGTCATGTTTTTTGCGTTCCTTGGGTTTTGCAGCTTGATCGCCACCACCATCTTCACAATCTGCTCTGCCAGCAGACACCCTAATAAAAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAGTAATAACAGTAATAATAGTAATAATATAGTTATCGTTTTTTGAAGGCAGAACACTTTAAGGAATAATATCACAAAGCATATACGGTAATACAATGCTTTGTTTTTTGTAGATCCCTCCTGTATTATATTATTACCTGGACTTCGGATTATCTTTGTTTTGCTAAAGAGAGTGCGAGATATGAAAACAAGAATGCGACTGCTTCCCAGATT

GGAGGCTGCTACGGATCATTATATATAATAAGATTCAAAGAGCAAATACTTTTGACAAAAAGAACAACAGAAATTTTAGAACGACTGGTGCATCTATACCTGGCCTCTTGGATATTAACCCCTTCAACTCACACAGAGGACAGCACTCCTACCTTGAAACCAGATTAGTCGACCCCACAATGCATGATATTGCTTTCCTCGGCCTTACGAGAGATGCCCGAATAACATCGACGACGGATAATCTATTTTTACTCTACAAGTACAAGGGTATACAAAGACCGTAGCTAATAATATATTTATAAGTAAACTTTCCCTTCTACCCGATACATACATTCAAAAAATCTAACGGGTAAAAATAAATTTCAAATAATTTTCCAGCGTGCGAAAATATATCTTCCATGGCATTAATCTATCTAATTTGATCCTAACAATTAAGGAGATTCAGCTTTTGAATCCAATTGATTTTATTACCTTTTTTTCCTAGTAAAAACAACCTGGACAGTAAATCGAATTGGAGGGGATTCTCCTTTACATGTACTTTCGGGATGTAATCATATTGTTGCTGAGAAAGTTATAGAACTTACTTTTGTTGATCGACTGGCGTTGTCATTTGTGAGGTATCAGCATTAACAGCTACTAGCTTCTTTTTGCGGCCTCTTTTTTTTGGCATTGGCTTGAAATTTATGCCGCACGAAGAGCTATCAGTTTGTTCCAATACAGTTGTACTTTGTTGACTGTGTCGATACATTTCTCTGCTTAGACATTCGGGAGTATCATTTGATACTAAAATCCCATACCGCCCTGGCCCAGTTGTGACAGCATCCAAATGACGATGTGATGGTGAATGCATGTGCAACTCCTCACCCATAAAACTGTAGGGATCGACAACAGACGCATGACTTGAGCATCGAGTGCCCTCACTCAGTTCTAGATGAGATTGGGAGGCGAGAACCTGTCCTTGACTACTGTTTGATGAGCAAGCATTGGGGTCAGTGTGAGCT

 

---

Search Again