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CRIMIC CR01850

Gene CG32017

CG32017

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	4:1137830..1162158	N/A

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAAATTGGGAGAAACGATCACACATT	ACTGGGCTTTGTAAGAGACGCTAC

Images

Construct Design Sequences

sgRNA sequence	GTTGGCATAAGTTACCGTATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCCACATGCCTTTTCTCAA	TATCGGGTTGGGGGTGGT	GCCAACAACGAGCGAAAGAG	TTAATGGACCACGCAGTCGG

 

Amplicon 5'

Amplicon 3'

CGCCACATGCCTTTTCTCAAGGCATCACTTCGGGCTTAGAACTTTAATTGGATTTAAGAAATTTCATGCATATCGACACGCGTTTTTATGGATTCATACTTCAGCCATGCAAGGAAAAAAGATTATCAGTATCACGCTCTGTACTTTTTGGCCTCCTTTAAATTAATTAATAATTTAATAAGTATTTTTACCTGTCTGTTTCGTTTCACCAACAGTATGGCAACCTTCGCGATAACTTTTGCCGACCTATTTAACGACCCCTTGCCATATCGAAAACTTTTGTTGGCGACGGCAAATTTTAGGTGGGGTCAGAAATAAATTTTTTATAAAATAACTAAGTTGCAGAATGATCTTGTGGTGATGAGGAATAAACATCAATAGACCACTAAGTAAACGATTTTTCCCCTTAGTTAACAAAATCTGAGTTGATATAAAAAAGGCAGATACTTTTCATTTTGGCCAGACAAAGGTCCCGTTTACAAATTGAAAAATGCAAATTCTTTTCAATTCGGCCAGACAAAAGTGCACGTTTTACGAATTTACGAATATTACGAATTCTAAAACCGGAGCACCGATTTCGCGATTTTAAATCGAAATAGTAAAATTCTTACAATTTTGATTACCGGAGCATGCCCAAATGAGTCAAAATAACATTTGCGCACAGCCTCAACGAGAGCAAGGTGAAATATCTCTTCGATCCGAAATATCACGTTTTTTTCTTTTGTAACTTCGCAAACACGCGGAAGTCCACGTAATAAATGCAAACGTCGAGCGGCCCCGGTCCACCACCCCCAACCCGATA

GCCAACAACGAGCGAAAGAGTGTAATCTTTCTAAAGCTATCAGGAAAACCGATGTTTCACCCTATTTACAAACTAAAATTCCTTCTGCATCGTGGAATTAACATAAATACATAATACAATACACTTTGAGGTTCTCATTTCACCCAACGATCACCTCCCAAAATGTGATTTTTCCAATCGTCCTGCAATCGAGACAAACAGAACCCAGCTCTACGACGTGTGCTCTAAAAGCTGCCCAAACAAATTAGACACCGAATCCTCAACGCAGTATTAAAACTATAATGTATACTCTGCAGCAGCATGTGATGCCTTTGCAGAACGAAATTTTCCTAATGGATCACAACGTTCAAAATAATCAATGGCTGCCTTTGATAATCATAAACTCGAAATTACTCACTTCCTACATGACAATCATTTTAACGTAAAAAAAATTAAAAAATTATTCAAAATTGTGCAAGTGACCGTTCTAAGCACGTTGTAGATGTTAGGAGGGGAGTGAACAAAGTATTTTTGGTAACCGGGGTTTGCATTAACTAAAAACTAAACTAAACTAAGAAATACACTTACGATCCCAGCATTCGAGAGTTCAAAAACAGAGGCTCCCAAACATTATATGTGAAGGGATCCCTATATGTTGGTATTTCATTGGTTATTTCTGCAGCTCGAGATTGCCTCTTTGAAAAACTATGTGGTACATGGAAAAAATTTTAATTTTTCTTAAAAGATAATAAAAGAAAGAACTTACATCCACATGGCGCGGAGCTGTTCGCATGTCGGTTCCTGTGAGTTAAGTGAAAAGGTTTTTGGCACAAACACTTCACCAACTGTTCTAACACCACTGGGATTTACGATATGTGATGCTTCTTCAGTTTTTACTCCATTGCTATTGCTTAAATTTCTTTCCCGACTGCGTGGTCCATTAA

 

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