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CRIMIC CR01852

Gene CG34443

CG34443

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14369067..14367979	50F6	FBti0213002	91329

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTGGCTTGCCACATCGCAATGTCT	TCTGATGATGGCCATGGTGATCACTC

Images

Construct Design Sequences

sgRNA sequence	TGACAATTAACACGCCTTTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGACACTGCCAGAATTGCAC	TGTCAACAAATGTGGCGTTT	ACAGTTCAACCAGAATGGGAG	CTCCACCCGTACACCAAACA

 

Amplicon 5'

Amplicon 3'

CGACACTGCCAGAATTGCACAGATTGGAAAATAGAGGGAAATATAAATAGCACCTCATCAAATAATAACTCAACTAAAGCTGCTTCGCGCGAAAAACGTGGTCTAACTCTGATGAGTCGCTCGGTATTTTATGCAATGCTAAGGGATAAATTAAGAAGGTCGGTGTCACATATATATATTAAAATATTTAATCGGTAAATTTACATATTTTAGGTCAGGGTTTCCAGCTGAACCCTGTTTGCTGCGCTTGATATGCGATACAAATGCTTCACAACTGGGTGAAGTTAATGGATTTTTAGGCAGCCTCGTTCACATTATATTCAGGTCAGTTGGAATCACTTTTTTAAGAAAGCCCGAAGATCATTCAAACTTCTCATTTAATTATAGTCCCAGCAGTTCCAAGGATGAGCACCTGCCCAATGAGTATTACCAGGCCGAATGGGATGGTCGAGAGCAGCAGGAGTGCTCCACGTATACCAAAAGTTGTGATCACAACATCTTGGACCTGGTTTCCGTGTCACTGGAACAGTCTTTAAGCGATATTGTAAGCCGACGAGGGCGCAAATAAACTGGAGTGTTGACCAAGAACCAAACCAAGTATCTTTCTGGGGGTTTACCTATCAAATTAGCAGCCACTTGCTTGGCGTTTGCCACGCACTTGTTGCGAGTGTTTCGAGTAGATTGCAATTCAGTTGCTGTCTGAGCATCGTGGTGGTCTATCGTCTGTCTCTGGGTCGGGAAAATGAATTTCACTAGTTACTTTCTTGGCCTGGTTTTCCTTTTAACCGTTTACCTCGACTTAACCAACTCCTTTGTGGCCTTTACAGCTTCCAGCACCCACGGTGTGAGTTCTTCAAGAATTTATTTAGCCGAAAAAGTACTACTATCATATTGTTTTTAGATCTTCGCCGCTATTGCCGTTCCCCTCGAGTTGCCGCATCGAAATGTGTTTGTCTCGTACAATTTTGAGGCCAACTACAATTTACCCGCAAACTGGGAGAAATGGACAATATTTGTAAGTTTCATTTTAGGTTTGTTTACGGCTATAAAACGCCACATTTGTTGACA

ACAGTTCAACCAGAATGGGAGTAATGTTTGGTTATTTACCCATCGCTCAAATTCCAGCAAAATGGTCCCATAGAGTCGGAGGAAGTTGTAGATGAAACCGATACGGAAACGGATCGCAAATTGGCCGCTGGTTGCCAAAACTGCACAGTGAAGGAGGAAAATGAGGCAGGTAGTGAAGAAGTGGAGGAAATCACCGAAGTTCTTCCCCAAGAACGAAAAGTTAGATCCTTGCTCACACGTTCCAATATCTATCGCATTTTCGTAGATAAACTAAAAAGGTTAGTTATTACTTATGGAATTGTGACTATACTAAGTTAGCTAAGTATTCTAGAAGTGGTTTTCGAGGTGAATCTTGCCTGCTTCGACTGATTTGTGAGACAAGTGCTGCTCAATTGGATGAATTCAACGGCGTACTGGGCAGTTTGATGCACGTTTTATTCAGGTATTATGAAGGCATCCTCTTAATGACCCAATATAACTTTTTAAATTTTAAAGTCCCAGCTCATCGGAGTCTGAAGATTTACCACTGCGCTATTACCAAGCGGAGCACGATGGTTGGAACGGTCACTGCCATGTCTACGAACCAGGTTGTGGCGAAAGTATTCTTGAGCTTATATCAGAACCTTTTGAAGATATTTTCAAGCACATAGAACGCAATAAAATATAGATAAAATATTCCTAAATCTGTTACTAGAACATACCATTTGGCCTGAATTGACTTACTGGCTTTTCAATTAGCAACTTTCGCTGCTTGAAACTAGAAATCATTAATTGGATCATCACTTCTAGCTAGAAAGAAAGGTTTTCACTTTGGCTTTCCAAAACTGACTCTTCAGTTGAATGAGGGTGGTTGGCTACAATGGATGGTCGTGGAACATTCCTAGTTCTTCTGATTTTTGGTTTTTTCAGGAACTCACAGGAAAAATTTGTTTGGTGTACGGGTGGAG

 

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