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CRIMIC CR01853

Gene Lst

CG8317

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16576586..16574579	53C8	FBti0213003	91330

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGATCTGATCCACTGATCACGTGCG	TAGAACTAGGACGCATCGAAAGTGGC

Images

Construct Design Sequences

sgRNA sequence	ATGCGTTCGGCCTTGTCCGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGTACGGCTTGGATCGCA	AGGCCGAACGCATTATCGAA	AAATCAGAGTACGCTGTCCG	AGCTTTACGACTCCACGGTG

 

Amplicon 5'

Amplicon 3'

AAAGTACGGCTTGGATCGCAGACTATCCGGCTGTGCCAACGGCGAGGGACTGTAGTTGAACTGATTGGGCGTCTCGATGTCCAGCAGGGCGTGGGGCACCTCCTGCTGCTGCGGTGCAGCCATTGTCGTCGTCATCGTTGTCGCCAGAATGACCAGGATCAGTGGAGCGAGCTGCAAGGAAGGAACAAGGAAGGAATGAAGGGAAGGGAACTTTGGTACACAAGTCAGTCAGAGGTGTGAATAGCTGATGGAGGCCAGCGCCAGAACCTGTCTGCTCTTAGCGCTCTTATGCTGCCAATTAGCATACAATAATTAATTTAAATTTAACCAACTGGCGGCGACGGGAAGATGTGATCCATGATATAATCGCAATCAAATCTAATTTATAGTCGCACGTAAAATATTCATGAGCACGTCGTCGCAATTTTGGTGGGCGTCATCGTCATCATCCAGCTGATCTCTAGGCGCCACCCAACCGGATGCATCACTGGCCATAGTCTCCTGGATCTCAAGCATTTGAATCAATCGCCAAGCCAAGTGTAATTGTTACTATGTTTTCGATGGTTTTTTTTTTCTTTTTGAATTATGTAAAACCTTAACCGCAGTCAAAAACAAATTAATTACAATCGGCGGCGGGGCAAAAGGAGAAGATCGAGATCGAGGTGGAGACTGTAACCGGTTGCGTTTCATGTGGTTCAGGGTTAAGAGAGCTCGACTTTGTAAACGGTATCAAGGCTCCACCAGCAGGATGGATGAGCTGCGGATGAGCTGCGGATGAGCGGATGAGCGGATGAGCCCAAGATCAAGTGCAAAGTGCCATGGGCACGAAATAAAGACATTTTACGACACTTCCTCATCGAGATGATCACAAGATCGAATCGAATCGAAATCGAAATCGAAATGGAAATGGAAACGGCTATAAGATATAGATGTAGAAATAGAATTGGAATAACTTGATTCGATAATGCGTTCGGCCT

AAATCAGAGTACGCTGTCCGTTTTGCAGCCTTAATATGTGAATTTTTTTTGGTTTTTGGTCGAAAATGTTAATCGGCGAAACACGTTTCTTTGTTGGCCTGTGTTGTGTTTTGGCTCATAAATTTCGAACTTTGAGTAACCATAATACTCACTTGCCAAGTGTACGCAAACATCTTTGCTGGGTATGCGTTAGTCGAGTTGCGTTGAGTGGAGTATCCTGTTTCGAGTCTGCACTTAGTCACTTTTTCCAATTCGGATGAAAGATTGTAGATTGCAGATTGCAGATTGTAGATTGAAGATTTCACTCGCGAATAGTCGAAACCGTTCTGATCGATCGATTAGTTAGTCAGTTTGTTTGTTATCAGTGATCGCTTAACACCTTGTTGGCCCCCGGGGCTATCGATTCGCTTTGGTTGGCTTCGATTCTCGTTGCTGTCGCCGCTGAGCGTGCGCTTCGACTGTTCAGAATTTTTGTGACAATTCACTTTTATACGGCCCGATTCGATGCCCTCGCCCCGCGGAACTGGTTTCCCGACCCGGGTTTCGCTAAATATTGCCGATATCGCGGCGTGCGTAGCAGCCCCCTTTGCCACCAAAAAAAAAAAAAATAAATAAATAAATAAAAAAAAAACACAACCTAGGGTTCCAGTTCCAGTGTTTTGTGGCCAAATTGCCAAATTGGTGGCACTGGCTGCATTTTGGCCGCTCCAGCTGGTCACGTAATTAACGCTTCTGCGGCTTCCCAGGGATATCAATTCAATTCAAATCAAATCAAATCGAATCGAATTGAATCACATATGCGTCACATAAGCCGCGTTTGTTTGCGCTTGGCATTAACCGATCTTCGATCTTCGGCGTTAACTAATTAAACTTTTTCCATGACGCACTTACCAATCAAGAAGACACTAATCTCCCCAGTAGTTCGGATAAGCAGATAGCAGGATGAGAATGGCGATGGGCAACTGGCGCCACACACACACACACGGAAGCCACCGTGGAGTCGTAAAGCT

 

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