CR01855

CRIMIC CR01855

Gene CG10479

CG10479

Information

Donor Location Cytology FBti Stock number
pM37-p03L:5967545..597600765A1-65A2 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK CGTATGTTTGGGTGGAACTGCAACCA CACAACTTTCCACCTTCGGAAGTGGT

Images

Construct Design Sequences

sgRNA sequence AGCGGAGTTTGGTTGGATCAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AACACGTCCCGCACACATAT CCAAACTCCGCTGGCTACAA GGAACATGGGGCTAGGAGC GGGGAAATCGGGCTGAAAGC
 
Amplicon 5' Amplicon 3'
AACACGTCCCGCACACATATCTCCCCCAACTCCCCCCATCATCTATGAACGAACAAATTCGGTTTTTCAGTGAGTTGGTTTTTCATTTAGTTGGTTAAACGAGTTCGTTTTTGGGACTCGTAAAATGATTTTCTCACTAATTTAGTTTAGTATGCCAGGAAAAATGTTTATAGTTTCAGCTTTTTGGTATGTTTTGTAAATTTTTTATTACAATTATCTTTGCCTTTGGCTGGAAAATCCTTTGCTTAGACACATGTGGCGTGTGGCATTGGCTTTTTTATGGGCCTCCCTTTGTATCTTTTTTTCTGTGGCATTTTTATAGGTGTAAAAAATTGGGTCATATAAAAATTTATTGAAATGTGAATCGAATTAAGCTGTTTCTTTTCAGCCGTTTGTTTTATGCGTGTTTAAAAACCATTTTGATGGGTTTATTAAATGCAAATGAAAATTGTTGAAATTTTAATTGCGTTTTATGGGCACATCGCTTTGGGGCTGCTTCTCTGCTGCGTGGGTTTTTCAAGAGATGAATCTCATGTTCTTTATATTTTTTGAAGAGCATAGGCAGGCAATGAACTCTTGGACTTTATCACTTTGAGAGATGGTGAACAATTTAATTAGAGACTCTGACAACTTTTTCTACAACTCCAATACGTCGGCTCTTGTGTGTGTGGAAAGTCTAGTAACTGGGCTTGCAAATAGTTTTCCCCGAGTCTAGTCGTCTAATGCACACAAATCCTTGGTGGGAAGCCATATGAAACTCAAACACGGACAACGGACTGGTGATAACGATGATGTTGGCTAGCTACTCGGTACTCGGTACTCAGTACTCCGTTCTCTGTTGCCGGCTGATGGCCAGACTAAGCCAAAACCAAAAACTATTTGTAGCCAGCGGAGTTTGG GGAACATGGGGCTAGGAGCTGAAAAAGAAGAGGTATTGAGTTGGGTTGGGCGGTTCGGATACCGTTGAAAGCTTTTTTGCATATTTGCATTACATTTTCATGGCGTGGCATGTGGCGAGCAAAACGAAGAGAAGCGAAAATTTATATTGAGTCGAGCGTAAAAATAACAATGCAGACAGTTGATGGTCGAGGCGGCAGACGGCATGAAAATAAGTGAAATTGTTGTTTACTCAAAGCGACGGGACCATTCGGTAGTTGGGCAAAATATGGAGCATAAATTACAATGCATGCAAGTCGAATGCAGTCGCCGGCTATTTAATTATGCTCATCAATTTGCGCAGCCTGCAGATTGCAGTTTCCCCAATAAATCTTGGGGCATTGTGAACAAGGCGTCTGCTTCGAAATTCCTAATTAAATGCACTTAAAAGCGATGCAATTAATTTTCTATCCTTAGCATTTCCTCTCCAGGGGCCCCCGAAAAATCAAAGAGTCTGCATGTAAATACATATGTAGAACACGAGCATCCAAATAAACATTAATCACTGCCCTTGAAAGGCAGTCCAGAGGCGAGTTCTTCGGGAAAAGTGCATTAAAATGCCTTAGAATTTAAATGCCTCAGCTCATTGCTGCTAATCGTCGCCAGCAGTTGATTTAATTTTAGCTGCATCTCATCGAGAATTATGAGGCCCATCACGGGATCTCGGCAGTCGAGGAAATCCACTTAGGCAGGGCGTTGAAGGCACCAGAGCATGGCATCATAAGCCACACGAGGAGGAAAATGCTTTCAGCCCGATTTCCCC
 
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