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CRIMIC CR01856

Gene CG17029

CG17029

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:15978435..15976870	72C1	FBti0213004	91331

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GACACTGAGAGCTGAGATAGAGCATC	CGTTGGAAACCTCAATGGGTTGTCCA

Images

Construct Design Sequences

sgRNA sequence	ATGACATTATTCCTGCAAACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTCTGCACATGAACTGCT	CAGGAATAATGTCATGCACGGT	CAGGTTTATCGGTGCACAGC	CGTACACGGTGACCAAGTCA

 

Amplicon 5'

Amplicon 3'

TCCTCTGCACATGAACTGCTCATATCAGCTCAGTTCATCCACTCACGAATTGTGAATTTATAACACACAAGATAGTCTCTAATAACCATCATACAACTTCAAGGAAACACACCTTTACATCTTTAAAATACGCAATGTATTATGAAAATATATCCTATGTATGATAGGTAATAGTAGAAGATAGATTGGGATATATCCTATATCCTATTGCAGTTATGTAGTATTAAATTCGTATATCAGAAAATATACTACAGGCTATGAAGATAAAAATGGTTTTCTTTAAATTCTATACCAATTTATACTCAATTTTATTAAAATAATAAAATTATCTTAAATAGTAAAAAGAATTTCCTATGCATTCATGGATTTTTAAATGTTAACAATAATAGTATTTTCTGATTCTTGGCTGACTAGTGTAGTAATATCACATCCATAAACTTGGAACAACGGTTCGTAAGCAACTCTATCGCGCTCTCCTAAATGTCCAAAGTTCTATTGCATCAATGAAAGCTTCGGCAGAAAGCTTTCCTCTGGCGTCGCCAACATTCCACAGCTGACTCGCGTCTATATAGTTCTGCTGCTCAGTCGGAAACCAACAGAAATAGCAATGAGTTCCGCGGTCAGCGAGTCCAAGCTCAAGGAGTACTACGATGTGGCCCTGAAACTGGTCCTCAAGTGCGGGCCTCTGATGCAGGAGGGCTATCAGAAGGCAAAGACGGAATACAAGGTCAAGGCGGACTTCTACGACCTGGTGACCGTGTACGATAAGCAAATCGAGGATATTTTGACCGAGGGATTGGTGGCCGCCTTTCCCGAATCCCTGATCATTGGCGAGGAGGAGTCGGCAGTTTCACAGCGCCAGGCGGAGCTGACTGATGCACCCACGTGGATCATAGATCCCATCGACGGCACCACAAACTTCATTCACCGCATCCCGCACTGCTGCATCTCCGTGGGCCTGGCCATCAACAAGGAGCTGGTCGTGGGCATTATCTACAATCCACCGGCGAATGAACTATTCTCCGCCTACAAGGGACACGGAGCCTATCTGAATGGCGAACCCATCCACACATCCAAAGTGACGACGGTAAGAGTGTGCTTATATAACCCGGGAGCAACAATACTATGTATAGCGACAAACCAGATAAACGAAATTTGACAAATAACCGTGCATGACATTATTCCTG

CAGGTTTATCGGTGCACAGCAAAAAAAAAAAAAAAAGGTTGGCTAAACTTAAATTTGCATTTCGATAGGAATGTTTGAGAAATTTGATAAATGTGATAAAATAATTTATTATAACCGCTTTATAACAAAATTCCCATACAATTGTTATAACAACTTTTTGGAATGGAAAAATACAATCTGCTAACATTTTTTTCGGTGCACCGATATCATTCATTCGTATACCACCAAACAAAAATAGCTCTCCAAAATTAAGCGCATATACAATTACTCTCACAACTAATGTACCCCTTTCCAGATCAAGCAAGCAGTGATTGCCTACGAGATCTCCCTGATCCACGCCGCCGGAGTGAGGGACAAGAATGTGAAGCGACTGTACAAGATGGCCTCGAATGCCACGGGAACCAGGTGTTTCGGAAGTGCCGCCCTGACCCTGTGCTACGTGGCCACGGGCCAGTGCGATGCCTATCATGTGGAGGATCTGAAACCGTGGGACATTGCTGCCGGAGCCATCATTCTGACCGAAGCTGGCGGCACTGTGTGCCATACCAGCGGATCCAAATTCGATGTGATGAAACCGGATTGCGTGTGCGCCGCCACGCCGGAGTTGGCCAAGAATGTGATTAGTCTTATCGAAGAGGCCGGTCAGATTACAGAGTACACATTTAAGTAAAGACAGTTAAAGGCTCAGTATGCAAAGACAGTATCCACATTTATCACTTGTAAACTATTCTAATCGCGCAGATATTCCAGTACAATAAAACGACCTAAGTACATATAAGTATCGCAGAACTTTCAGGAGCTTGCGAAGAAAGCTTTACCATAATGTCAGCTGTGTTTCTAAATTAAGTATTAGTTTCTACTCTGATCAGTCGATAGACGAGCCATGAGTTACCGAATCGGCGAGGAAAAACTTGAAGTATATTACCAGGTATCCCTGGAACTGGTCAGGAAGTGCGGTCCGCTCTTTTTGGAGGGTTTCCAGAAACCGAAAACCGATTACGAAGTCAAGTCAGCGTTCTATGACTTGGTCACCGTGTACG

 

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