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CRIMIC CR01859

Gene CG13532

CG13532

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22874427..22871190	59B4	FBti0213005	91332

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GGTGAGCAGTTCTCGCATACATCCTT	TTGCATTCACAGTCAGACGGTGAAGC

Images

Construct Design Sequences

sgRNA sequence	GTCTTTCGGCTCCTGTCCGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGCCTGCAAATAATCGGTG	GACAGGAGCCGAAAGACAA	ACCCTTCGTCCAATCTGCTC	AGGCGGTTGGTTTAATGCAC

 

Amplicon 5'

Amplicon 3'

CCGCCTGCAAATAATCGGTGGGTGTCACGACCACGGCCAAAAGAAAAAGGATCGGCGGTGAAAGATGTGCGGTGGCGATGGGAATGGGAATGGGTAGCAGGTGGTGGTGGGGGTCGAAAGGTCTAAGGTGGTGGTTCTGCCTCTGGTTCTGCCGGGTAACCTGCACCCGCCCCCATCTTGACTTGGCCCCCGCCACCAGGGTGTTTGCATAGGTGTTCTCGTGTCGGTGTTTACACAGGGAGACAAAAATTATGATTTATGTACTTAAAAGCCATTAACAAAAGCCGAGAAAGCCTTACTCTAATGCGCTGGCCTTTATTCTGTCCCACTATTAGCATTCATTCTCTCGCATATCATCTTGTGCCTGGGCAATTCTAATTTAAATTTTCAACGTACCTTTTTAAGGAACTTATATTCTATTATATTTCATGATATTACTTTCTATTTTGTTTTAAAGCAACTTTGTTAGCTATATATATATATATCCATAACTACGGTTTGAATATTTTAATCTTAGTTAGTATCTGAAGTTGTTTTCAGTGCTGTAGGACAACTGTAATTGGCTTAGTCAGGGCGTTCCCAATCGAGTTCCCCTTTTAAATGCTAGGGTCAGTACTTTTTTTTGCTAGTGCAGGTGCTGGGCGCCGTCTGTGTGGCACGTGTGATTCGCTTTCATATTTGCATGCGTGTCATGTGGCAGTCTCTGCGGTGTCCGGAACCACGGCTTGTTTGTGCTGTTTTGTGCTTTTCTTTGGGACACTGTTTTGCTTGGTATTGTTTTGTTTCGTTGTTTAGTTTGACACTTGATTAATTCTCACGACTTTTTGTCTTTCGGCTCCTGTC

ACCCTTCGTCCAATCTGCTCCAATTGCGACAGTTCCTGAATCGGATTTCATGCTGGAAGCGCGGATGAGCGGTTCGCGTATGGACGTGGACATCATGTGTGCGGTCCAGCACGTCTTCCCGCAACCCATGCTGTCTGTCATGTAAGAGGATCCACTGTCCCCGGCAATTTAAGGCCGGTGAAATCCAATAGCGCAAGGACCTAATCGTTCCCTCCGCAGGTTTGACACCCACATACTGGACTCGGTACTGACGCAGTTGGACCAGGACCCCAGTGGCCTCTACAGCATGACCGTTCGCACCCGCATTCCTAGAGATCAACTCGAGTCCCCCACCCCAATCACCTGTGCGTTTATCCTAGTTGGCACCAACTACACCAAGCGCCGGGAGACTATTTTCTATGGTAAGTACAGGTAAGCCTGGATAACACTTTGCTTATAAGGACAAGTTATTCATTCATGCATCATAGCATTAAAGTAGTAAGCTAGATTTGTTTGAAATTTTTAACATCTGTCCGTTTCTCCATCCTTATAGAAAACCTAGTTTTAAAGTTAACAAAATTGAATACATAATCTAATATTTTCTTCTTTACAGATAAGGCCTCAACTATACAACGCAAGTGGACAACGGTCGCCGTAGTCATGTCCATCGCATCCTTAGCTCTAAGCAGTTAGTTTGGTAGTGTCCTTTTTATGTAGGCTACATTCGTTTTTATGTTTGGACTAGGAACTTTGATACTCCTTAATTTTTAATAGACTTTTAACGGATAAACGTAATTTTTTGCGGTATTTTTAAAAAAAAGCGCTTATTTTGTACATGCATGTATTTGTAAGGATAACGAAACACAAAACAAAAACAAACACCACAATGCGTAAGCAAACGAACCCATAAAGACAAAAAGGACAACAGTAAAGCATAACAAGAAATTCTTGTATTCATATATTTACCACATAAATAAAATATATATGATAGTTATATACGATGTATAGGTCTAATTGAGTACAGAAACGCTAATAAAAAAAATACGAAAAGAAACATTAAAAGTGCATTAAACCAACCGCCT

 

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