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CRIMIC CR01862

Gene gry

CG17569

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:3211348..3199090	63B11-63B13	FBti0213021	91335

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGGATCCAATCCTGCGTAGGGTTT	AGGTCACCACATCAGAGGAGCTATGT

Images

Construct Design Sequences

sgRNA sequence	CAACTGGAACCAGTGTTTGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGGTTATTCTCGCGGGTA	AGGAGGCCAACGTTAAGCTC	CCAGTTGTGACCCTGCTCAT	TCGCGCTTTGGTCAAGGTAT

 

Amplicon 5'

Amplicon 3'

CACGGTTATTCTCGCGGGTAACTCCCCAACCACTGATCCTGGCATACGCATTACCCTCCGGCGGATTGCGACAAGGACTAATGGTGGCGACTTTACCGGGGGTTAGGACCACGACTTCCTGCAACTGCAACAAAGCTACGTCCATGTCGAAGTTAGTGGCAGAGTAGCTGGGCGAAGTGTGAAACATAACTACATTGCGAACCACTGGAGCCTCTTGGTCCAAACGACTCGCGCCGGCGTGAACCGTGAATCCCTCCGGCTGCGATCCATAAACACAATGTGCTGCACTTAGGACTGCTCTGCTGGAGATTAAGGATCCTCCACAGATAAAGTAACCATTTTGCCGCAGGTAAACCAGGTACGGAACTTCCGAAATAGTTGTCTCCTTGCCGCCAACTATGCGCGTTTTGCTAGAGGAGCTGGGAAGTACAAGTGCCAAATGGCAGAGCCACCAGAGCAACCTTAGGCCAACCATGGCGTGTCCATACTGACAGCTACTATGTACACATGTATATTGCTTCCTAGTTTTCAAAGTGATAATGATGCTGTTATTGGCTCGAACCTGTCCCGGAAATGGGACTAATCGCACTATTATTTGCTATTCGGAAAGAGATCTCAATTGTAATATGGAGTGACTGCCTTATTAACTATTTATTGCGTTGAAGGTTAATCAAATTGATTGATAGATTTTTAGCAATGTCGCTCGATAAAGTCCTTCAGCCAGTACGTAATCGATGGACTACTGAGGCGGGTGTAGACGCCTGGATAACCACTGCCACATCCGTAGCCCCACGATACTATGCCCACGGAGCGACCAGCGTAGATTGCGGGGCCTCCGGAATCGCCTTGACAGGCATCCCTGGCGTTTTTGCCCAGAGCACAAAACATGTTGTTCGTTACTTTTTGCCATCCGGATCCAACTGATTTGATGCACTCCCTGTGCGAGATGAGCTTAACGTTGGCCTCCT

CCAGTTGTGACCCTGCTCATTGATCGCTCCCCAACCCAGAACCGTGAGATTTGAATGTGGTGGCAGGTCATTGTAGTCGATCTTCACCAGACTCGCATTGCCGGCGATGTCAAAGGGACGACTCAAACGGATCACCGCCAAGTCGGAGTCCAATCCGCGTTGAGCGCAGTAGTTTGGTGATAGGCCGACATACCAGGCCGAACGGACATGCTCCGGCGGCATGTCATCGCCAAGGCACTGTTGTTGGGCATGTACGGTCAAATCCCGGACCTGTTGATATCGTCCCTCCAAGCAATGAGCAGCTGTGAGTACACAGTTGGGCGAGATTATGACGCCACCGCACCGGAACTTGCCACCTCGTCGAAGATTGACAAGGAAGGAATGGTCTTTCACCAACGTGGTTTTTCCTCCGAAGATTTTACCCTGACGAAGAGTGAATAGTGAGCTGCCCTCCAGTTGATGGAGCAGTGCTAGTCCAATAAGGAGTATATACATGGTCTACCGAAATCTAAGCCGGGAAGCAAGTTTATATATATTATTATATATTATCTTTTCCCGCATTCCCCAGTTAATTGCCTTCAGATAAACTTGAACTATTTATTGACTCTTAAAGCTAAGACTTAAGATTATGCTCAGGAATGCTGCTCGACGACGCGCTGAAACCAATCCCGGATCTCAGGCACACTGGTGTATATGCCAGGCAAAGCGGAGGCACATCCATATCCAAAGGATACGATCCCCGCCAGCTGGCCGCGATACACCAAAGGTCCACCCGAGTCGCCGTCACAGGAATCCTTCTTGTACAGCCTGGCGGCGCACACCATGGAGCGGGTAATGCGGTGGATTCCTCTGAAAGCAGACACGCAGGATGCACGCGACCACATGGGCACCAGCACACTGTGCACCCGCTCGGCAGTCTTTGTCGCATCGGCAGTTAGGAAACCCCAACCAGATACCTTGACCAAAGCGCGA

 

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