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CRIMIC CR01863

Gene CG12974

CG12974

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:21281752..21285962	78C4-78C5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGTCTCTCCCTATCGAATAGTCCAGT	CACCACAATGGTCTCCATGGGATTCT

Images

Construct Design Sequences

sgRNA sequence	CATTACGGGCAAGTGCTGTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGGCATTGTTATACGCCTG	AGCACTTGCCCGTAATGGA	ACAGGGCGCTAAACATGTGA	CATCTGGATGGGCTTGACCA

 

Amplicon 5'

Amplicon 3'

GCGGCATTGTTATACGCCTGGACCTCCACCCTGTCCATACAAATCGAGAATTATTTTCAAGACATTAAGGATGTGTGAATCGTTATACTTACTCCTCGTAGGCGCGAATCGGGGTCCTGGTGGTCAGAATATCATTGGAATCCTTGCGAGGATTGAACAAAGTATCCTGTGGAAAAGGGCATGTTTGTTTGATTTTAGGCAAGATAGCTATAGCTCATTCAAGATTTTGTAATTAATTTATATTAAGGAAGATATTTGAGGCTTTTTAAGGATACTTTAAGTGTACACTGAGTTAAGAACACCTCAGTTCAAGCGAACTGTCCTTGCAAAGAACCCCCGCACCTAAGGCCCAGTAAGTCGTCATTTTCCAGCGTGCTCGGTCAAACCAAACCGAAAAGCCAAGAGCCCGCTATCAGGCAAACCTGGTGCCAAATTGGAAACCAGCTTATCAGCAATTACCACCACCACATTGAGTTAATGGCGACAAAGTGCTAAGTTTAGCCATGTCGTAGTCGCTGCCCGGCGATTTTTAACTTCTACGTTGTTGGAATATTTTCAAAGGCAGTGTCGAAAATTTATGGAATCAGCGGAGACAACGAAGAAGTCACTCAGCATTGGTGGGCGATGAGCTCACTCGCCGACCTGACCACAACTTACCACATTGTCCTTATCGGTGGAAATTCGAATGGCTCTCGCGACAAGGTATGCGAGCAGTCCAAAGTTAATGAACCACAGAACGAATCCCTTGAAGGAGATGACCATCATGGAAATGGCAGCCACTCCTCCGGCGATCAAGGTGGCAGCCAAGGGATAGATGACACCAGCATTGATACTATTGGCATTATACCTCAGAGCCTCCTTGTGGAAACGTCCGTAGTCCACGCCGAAGATCACTCCAGAAGCTACATCCATGCAGCATATGGTGGCCACGAAGAAGGCCCAGATGTAGATGGCAGTCACGGTCTGCTTCGGGTTGTCCTTGCGCACCCCTGAAATGGTAAGAGTATAAGCGGGTTATTAACTTAAGTGTAATCCATTACGGGCAAGTGCT

ACAGGGCGCTAAACATGTGATACTTAAATGGGCTTACTTATTGGCGATACATCAATCAATCTTATAATGGAGTCATTAGGTAATATAATTTTAAAGACCATTATGTACGAGTAAAGTATGCTCTAAAATGTGTCAAAAAGCTAATCACTTTGGTTTCAAAATTAAAGTAAATAGCACTTAACAAATTTTAATTAATCAATTACTAGATAAGATATGTTTCGTACATATTTGAGCAGTATTAAGATTGAGTCATAACTATAACTATAACCATGCTACTATATTTTAACTTTAGAAAACGAATTGTATGAATGAATATATCTGTCTATAACAAAGAATAAGACTTTCCAGTTTACTTGTGGGCTAATGTGCTTAATTCTTTGAAAGCCAATAGCAAATGCTGAAAATTGTATATGGTGGGAAAGAACCATTAATGAAATCGTTATATAACCCATCATATAAGTTCCCAGCGAACTTATCATCAAGTTTTGTTTATTTGGGCTTTACGGCTGTGTGCCATGCGTAAGTTTTGGATGCCTCACAAATAAATTTCTAGATTTCTCCTCGTGAATCATAAAGGCTTCGATATCTTAACTATGTATGCAGTTCATTTCCGGTTCTTAAAGTACTTACACAGTAGCAGCACAACGGAGACGATTAGCCAGCAAACCGAAACGCCCAGGTAGACCGAATTCCAGATCAAAATCTGGGTGGGATCAAAAACAGTAGTCACTGTATTCAGGATGGTTTGGTCGTAGTTCTGGTAATCACTGGGATTCATCTTGCAACTGCCCTTGAAGTACACGTCAAAGAATACAGTCTTGACCATGGAGCCATAGGTGAGGTCATTTGTAATATACAGGACACAATTGTAGGCACATATCGCTGTTATGGTCAAGCCCATCCAGATG

 

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