• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01867

Gene RhoBTB

CG5701

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:20382398..20376242	77B6-77B9	FBti0213008	91336

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTTGTACAGGGAGCTGCATCATCCG	GAGCCAGGAAGATGATCAACTTGAGC

Images

Construct Design Sequences

sgRNA sequence	TTGGACTGGGATGTTAAGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACGGCATTGCATTGGTGAC	TGGTGGGATGTGGAGCAGAT	TCCAGTCGGAGGATCAGCTC	CTTCGGCTGTCCACATTCCA

 

Amplicon 5'

Amplicon 3'

AACGGCATTGCATTGGTGACGGGTGCTTCAGTGATGTCACCTTTGAATTGGACGATGGCTTAATGAAGGTACATACATATGTCTGTTGAATTACTATATGAAGCACAATCGTTTACACTTACCTTTGCTGCATTGCAGGCACACCGCGCTGTGTTGGTTGGTCGTTGTGATGTCATGCGCGCAATGTTGTTAGGCGACTTTCGCGAGGCTCATTCCAATGTGGTAGGAACGGGCACAATACCCCTTAACTAAGGACAGTTGTCATAAATTTCCTTTACCTTAGATCGTATTCCCTGGCGTTACCATTTACACATTCCACAAGCTGCTGTGCTATTTGTACACAGACCAGATTCCGCCAATCTCGGCCGTCAAGTGCCTCAATCTGCTGGAGCTGGCCAATAGACTCTGCCTGCCGCGGCTGCTCAACCTGGTTGAATGCCGCGTTATTGAAGATCTCACTCTGATTTCTCAGAACGAAACCAATGAAACGGTGGATCACTGTCTAAAGCTGCTCGAGCCAGTGAAGGTACACATAGAGAGATCAGTTAAAAATGATATACCGAAATTCAATACTTTTCTTTTTTTTTCAGCTGCACAACGCACATCAATTGGCCGAGTGGTGCATGTCATACCTGTGCGTCAATTACAACCTTATTTGTAAGTTTTCACTCAAGGGCCTAAAGGCGCTACACCAGGATAATCAGGAGTACTTGCGCGAGCACCGATGGCCTCCAGTCTGGTATCTGAAAGACTATGATTACTATCAGCGATGCCTGAACGAGCTGAACAAGGAGCTTAAGCTAAAGACTTCGAGGCGGGAATCGCCTAGCGATGACGAGGGCTGTCTGTGCTTTACGGGCGGTAAGTGTTGCTACATTTACGGAACTATCTGCTCCACATCCCACCA

TCCAGTCGGAGGATCAGCTCAGGTTTTAGTTTCAGCTAGCTTTAGAGGACAATGTATGTATCATGACGGCGGTTTGTATTCGTAGCTAGGTGTCTCGTTATTGTTTTCCTTTCCTGTTGATTTCCTTGCTCATTTACAACGACAACGCCATCATCATAATATTAATGTCCGAAATTTGGTCAAAGTATTCTCTTGCTGGCTGTTAGGTAAATCGAAGCGAAGCGCTGATGTGAGTGGAACCACGGATAACAGCAACGCAGATAATCAGATCTTCAACAGCGCCGGCAACTCGCTCAACCACATTGATCTGGAGGCGGACATGGACCTGAATCTCTGACACCCAGCCCTAGGGCAAGGAAGGTGTGTTGATATTAATGTCAAAGTAAGGATTCTTCGATATTTATGATTTACTTCGATACTTTGATTCCAGATCACTGCGCTTCATTGTGATCCTGCCGGTCCTGATCTGTTTTATCTGTACGATTTTAAGTATTTTGATACTTTTCCAAATCCATACATAACAAACCTCAAGGGCACGATTTAGAGTGGTCGGGGAGATAAGGGTTGGAAATGGAGGGGGCGTGGCGCGTCTGCTTTTGACATTACGCAATATATAGAGCGCAAATTTGCCTTAGTGATTAACGATTAATAAGTCCAACGTAGCTCGACCCAGAACAAGGGAGCATACTGCAGAGTGCTTCGGATTTCTTATATTTATACGCCTATATAAAACGCAAAGCATAGACGAGTATGTATGCACATGCACCTCGGCGCTGCGAAGCATTTCGCTGTATTGCCTTACGATATATCGGTGTACACTCAACTTGTTCTGCACGATCCCTCCCATGGAATGTGGACAGCCGAAG

 

---

Search Again